Rabbit anti-Human CXCL1 Polyclonal Antibody | anti-CXCL1 antibody

Anti-CXCL1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8100209

• Gene Names: CXCL1; FSP; GRO1; GROa; MGSA; NAP-3; SCYB1; MGSA-a
• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: CXCL1; Polyclonal Antibody; Anti-CXCL1 Antibody; Rabbit Polyclonal; CXCL1 Antibody; Rabbit PAb; Antigen Affinity Purified; chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity; alpha); Anti-CA21H Antibody; Anti-CAH1 Antibody; Anti-CPS1 Antibody; Anti-CYP21 Antibody; Anti-CYP21B Antibody; Anti-FSP Antibody; Anti-GRO1 Antibody; Anti-GROa Antibody; Anti-MGSA Antibody; Anti-MGSA-a Antibody; Anti-NAP-3 Antibody; Anti-P450c21B Antibody; Anti-SCYB1 Antibody; anti-CXCL1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human CXCL1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CXCL1 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:1000; IP: 0.1-0.5uL/mg of lysate
• Immunogen: A synthetic peptide corresponding to the center region of the Human CXCL1
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human CXCL1, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunoprecipitation (IP)

(ASF1B was immunoprecipitated using:Lane A:0.5 mg K562 Whole Cell Lysate0.5 uL anti-ASF1B rabbit monoclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-ASF1B rabbit monoclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 22 kDaObserved band size: 22 kDa)

Western Blot (WB)

(Anti-CXCL1 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:11 kDaObserved band size:13 kDa(We are unsure as to the identity of these extra bands.))

Related Product Information for anti-CXCL1 antibody

The Chemokine (C-X-C motif) Ligand 1, CXCL1, is a small cytokine belonging to the CXC chemokine family that was previously called GRO1 oncogene, GRO?, KC, Neutrophil-activating protein 3 (NAP-3) and melanoma growth stimulating activity, alpha (MSGA-a). CXCL1 already known to be important in osteoarthritis (OA), as a novel target gene of transcription factor AP-2? in chondrocytes and support the important role of AP-2? in cartilage. CXCL1 is a potent neutrophil chemoattractant with recognized roles in angiogenesis and inflammation. CXCL1 is a novel immediate PTH/PTHrP-responsive gene. CXCL1 may act as a chemoattractant for osteoclast precursors. CXCL1 may also have important pro-nociceptive effects via its direct actions on sensory neurons, and may induce long-term changes that involve protein synthesis. CXCL1 plays a critical nonredundant role in the development of experimental Lyme arthritis and carditis via CXCR2-mediated recruitment of neutrophils into the site of infection. CXCL1 functions through CXCR2 to transactivate the EGFR by proteolytic cleavage of HB-EGF, leading to activation of MAPK signalling and increased proliferation of epithelial ovarian cancer (EOC) cells. It might limit tumor growth by reinforcing senescence early in tumorigenesis. Thus, CXCL1 plays a role in spinal cord development by inhibiting the migration of oligodendrocyte precursors and is involved in the processes of angiogenesis, inflammation, wound healing, and tumorigenesis.

Product Categories/Family for anti-CXCL1 antibody

IL17 signaling pathway

References

Wang JG, et al. (2008) The chemokine CXCL1/growth related oncogene increases sodium currents and neuronal excitability in small diameter sensory neurons. Mol Pain. 4: 38.Acosta JC, et al. (2009) A role for CXCR2 in senescence, but what about in cancer? Cancer Res. 69(6): 2167-70.Onan D, et al. (2009) The chemokine Cxcl1 is a novel target gene of parathyroid hormone (PTH)/PTH-related protein in committed osteoblasts. Endocrinology. 150(5): 2244-53.Ritzman AM, et al. (2010) The chemokine receptor CXCR2 ligand KC (CXCL1) mediates neutrophil recruitment and is critical for development of experimental Lyme arthritis and carditis. Infect Immun. 78(11): 4593-600.Bolitho C, et al. (2010) The chemokine CXCL1 induces proliferation in epithelial ovarian cancer cells by transactivation of the epidermal growth factor receptor. Endocr Relat Cancer. 17(4): 929-40.Wenke AK, et al. (2011) The transcription factor AP-2? regulates CXCL1 during cartilage development and in osteoarthritis. Osteoarthritis Cartilage. 19(2): 206-12.

NCBI and Uniprot Product Information

• NCBI GI #: 4504153
• NCBI GeneID: 2919
• NCBI Accession #: NP_001502.1
• NCBI GenBank Nucleotide #: NM_001511.3
• UniProt Accession #: P09341; Q9UCR7
• Molecular Weight: 11,301 Da
• NCBI Official Full Name: growth-regulated alpha protein
• NCBI Official Synonym Full Names: chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)
• NCBI Official Symbol: CXCL1
• NCBI Official Synonym Symbols: FSP; GRO1; GROa; MGSA; NAP-3; SCYB1; MGSA-a
• NCBI Protein Information: growth-regulated alpha protein; MGSA alpha; GRO-alpha(1-73); C-X-C motif chemokine 1; fibroblast secretory protein; neutrophil-activating protein 3; melanoma growth stimulatory activity alpha; GRO1 oncogene (melanoma growth-stimulating activity); GRO1 onc
• UniProt Protein Name: Growth-regulated alpha protein
• Protein Family: Growth-regulated alpha protein
• UniProt Gene Name: CXCL1
• UniProt Synonym Gene Names: GRO; GRO1; GROA; MGSA; SCYB1; MGSA; NAP-3
• UniProt Entry Name: GROA_HUMAN

NCBI Description

This gene encodes a member of the CXC subfamily of chemokines. The encoded protein is a secreted growth factor that signal through the G-protein coupled receptor, CXC receptor 2. This protein plays a role in inflammation and as a chemoattractant for neutrophils. Aberrant expression this protein is associated with the growth and progression of certain tumors. A naturally occurring processed form of this protein has increased chemotactic activity. Alternate splicing results in coding and non-coding variants of this gene. A pseudogene of this gene is found on chromosome 4. [provided by RefSeq, Jan 2012]

Uniprot Description

CXCL1: Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO- alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q21

Cellular Component: extracellular space; extracellular region

Molecular Function: growth factor activity; chemokine activity; CXCR chemokine receptor binding; enzyme activator activity; receptor binding

Biological Process: positive regulation of catalytic activity; negative regulation of cell proliferation; cell proliferation; nervous system development; G-protein coupled receptor protein signaling pathway; immune response; response to lipopolysaccharide; positive regulation of leukocyte chemotaxis; inflammatory response; actin cytoskeleton organization and biogenesis; chemotaxis; signal transduction