Rabbit anti-Human STUB1 Polyclonal Antibody | anti-STUB1 antibody

Anti-STUB1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8106493

• Reactivity: Human
• Applications: Western Blot, ELISA, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: STUB1; Polyclonal Antibody; Anti-STUB1 Antibody; Rabbit Polyclonal; STUB1 Antibody; Rabbit PAb; Antigen Affinity Purified; STIP1 homology and U-box containing protein 1; Anti-CHIP Antibody; Anti-HSPABP2 Antibody; Anti-NY-CO-7 Antibody; Anti-SCAR16 Antibody; Anti-SDCCAG7 Antibody; Anti-UBOX1 Antibody; anti-STUB1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human STUB1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-STUB1 antibody: Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10,000; IP: 0.5-2uL/mg of lysate
• Immunogen: Recombinant Human STUB1 protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human STUB1. STUB1 specific IgG was purified by Human STUB1 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunoprecipitation (IP)

(STUB1 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg 293T Whole Cell LysateLane C:0.5 mg MCF-7 Whole Cell Lysate2 uL anti-STUB1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-STUB1 rabbit polyclonal antibody,at 1:1000 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 35 kDaObserved band size: 38 kDa)

Western Blot (WB)

(Anti-STUB1 rabbit polyclonal antibody at 1:500 dilutionLane A: 293T Whole Cell LysateLane B: MCF7 Whole Cell LysateLane C: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:35 kDaObserved band size:35 kDa(We are unsure as to the identity of these extra bands.))

Related Product Information for anti-STUB1 antibody

CHIP, the protein encoded by STUB1, is a central component of cellular protein homeostasis and interacts with several key proteins involved in the pathogenesis of manifold neurodegenerative diseases. Missense and truncating mutations in STUB1 lead to SCAR16. CHIP/STUB1 ubiquitin ligase is a negative co-chaperone for HSP90/HSC70, and its expression is reduced or lost in several cancers, including breast cancer. Pathogenic variants in STUB1 were initially described in autosomal recessive spinocerebellar ataxia type 16 and dominant cerebellar ataxia with cerebellar cognitive dysfunction (SCA48). Data indicated that STUB1 deficiency leads both to the intracellular accumulation of protein aggregates and to an increase in the secretion of small extracellular vesicles (sEVs), including exosomes. Secreted sEVs are enriched in ubiquitinated and/or undegraded proteins and protein oligomers.

Product Categories/Family for anti-STUB1 antibody

Adaptor Proteins

References

Roux T, et al. (2020) Clinical, neuropathological, and genetic characterization of stub1 variants in cerebellar ataxias: A frequent cause of predominant cognitive impairment. Genet Med 22 (11): 1851-1862.Luan H, et al. (2018) Loss of the nuclear pool of ubiquitin ligase chip/stub1 in breast cancer unleashes the mzf1-cathepsin pro-oncogenic program. Cancer Res 78 (10): 2524-2535.Hayer SN, et al. (2017) Stub1/chip mutations cause gordon holmes syndrome as part of a widespread multisystemic neurodegeneration: Evidence from four novel mutations. Orphanet J Rare Dis 12 (1): 31.Ferreira JV, et al. (2019) Exosomes and stub1/chip cooperate to maintain intracellular proteostasis. PLoS One 14 (10): e0223790.