Rabbit Dact2 Polyclonal Antibody | anti-DACT2 antibody

Dact2 Antibody

Cat. Num. AAA150212

• Gene Names: DACT2; DPR2; DAPPER2; C6orf116; bA503C24.7
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Dact2 Antibody is affinity chromatography purified via peptide column.
• Synonyms: Dact2; Polyclonal Antibody; Dact2 Antibody; DPR2; DAPPER2; C6orf116; bA503C24.7; PP13671; Dapper homolog 2; Dapper antagonist of catenin 2; dapper; antagonist of beta-catenin; homolog 2 (Xenopus laevis); anti-DACT2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Dact2 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 774

• Applicable Applications for anti-DACT2 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Dact2 antibody can be used for detection of Dact2 by Western blot at 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: Dact2 antibody was raised against a 12 amino acid synthetic peptide from near the amino terminus of human DACT2.
• Buffer: Dact2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: Dact2 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of Dact2 in SK-N-SH cell lysate with Dact2 antibody at 1 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of Dact2 in human brain tissue with Dact2 antibody at 2.5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of Dact2 in Human Brain cells with Dact2 antibody at 20 μg/mL.)

Related Product Information for anti-DACT2 antibody

Dact2 Antibody: The Wnt signaling cascade is a conserved process in multicellular animals that plays important roles during development and can contribute to cancer and other diseases. Many members of this pathway are also expressed in the postnatal tissues such as brain. One such protein is Dact2, a member of the Dact protein family that was initially identified through binding to Disheveled (Dvl), a cytoplasmic protein essential to Wnt signaling. Dact2 is most prominent during the development of the thymus kidneys, and salivary gland. Dact2 is thought to play a role distinct from that of Dact1 with Dact2 having a greater impact on a beta-catenin-independent process termed planar cell polarity/convergent-extension signaling. Furthermore, Dact2 but not Dact1 can inhibit Nodal signaling by promoting the endocytic degradation of TGF-beta receptors. At least two isoforms of Dact2 are known to exist.

NCBI and Uniprot Product Information

• NCBI GI #: 163965434
• NCBI GeneID: 168002
• NCBI Accession #: NP_999627
• NCBI GenBank Nucleotide #: NM_214462.4
• UniProt Accession #: Q5SW24; Q2NKJ2; Q569G0; Q8WYW2
• Molecular Weight: 29,350 Da
• NCBI Official Full Name: dapper homolog 2 isoform a
• NCBI Official Synonym Full Names: dishevelled-binding antagonist of beta-catenin 2
• NCBI Official Symbol: DACT2
• NCBI Official Synonym Symbols: DPR2; DAPPER2; C6orf116; bA503C24.7
• NCBI Protein Information: dapper homolog 2; dapper antagonist of catenin 2; dapper homolog 2, antagonist of beta-catenin; dapper, antagonist of beta-catenin, homolog 2
• UniProt Protein Name: Dapper homolog 2
• Protein Family: Dapper
• UniProt Gene Name: DACT2
• UniProt Synonym Gene Names: C6orf116
• UniProt Entry Name: DACT2_HUMAN

Uniprot Description

DACT2: Involved in regulation of intracellular signaling pathways during development. Negatively regulates the Nodal signaling pathway, possibly by promoting the lysosomal degradation of Nodal receptors, such as TGFBR1. May be involved in control of the morphogenetic behavior of kidney ureteric bud cells by keeping cells epithelial and restraining their mesenchymal character. May play an inhibitory role in the re-epithelialization of skin wounds by attenuating TGF-beta signaling. Belongs to the dapper family. 4 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 6q27

Cellular Component: mitochondrion

Molecular Function: protein kinase C binding; protein kinase A binding; beta-catenin binding; transcription factor binding

Biological Process: skin development; hemopoietic progenitor cell differentiation; negative regulation of cell adhesion