Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Dapper homolog 2 (DACT2) Standard Curve.)
Mouse Dapper Homolog 2 (DACT2) ELISA Kit | DACT2 elisa kit
Mouse Dapper Homolog 2 (DACT2) ELISA Kit
Gene Names
Dact2; Dpr2; Frd2; dapper2; A630024E20; 2900084M21Rik
Reactivity
Mouse
Synonyms
Dapper Homolog 2 (DACT2); Mouse Dapper Homolog 2 (DACT2) ELISA Kit; DACT2; C6orf116; DAPPER2; DPR2; FLJ31232; MGC133141; MGC133142; RP11-503C24.7; bA503C24.7; dapper homolog 2; antagonist of beta-catenin; dapper; homolog 2; DACT2 elisa kit
Reactivity
Mouse
Specificity
Mouse Dapper homolog 2 (DACT2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse DACT2. No significant cross-reactivity or interference between Mouse DACT2 and analogues was observed.
Sequence Length
757
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Dapper homolog 2 (DACT2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Dapper homolog 2 (DACT2) Standard Curve.)
Related Product Information for DACT2 elisa kit
Description: This Mouse Dapper homolog 2 (DACT2) ELISA Kit employs a two-site sandwich ELISA to quantitate DACT2 in samples. An antibody specific for DACT2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DACT2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DACT2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DACT2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: DACT2 (Dishevelled Binding Antagonist Of Beta Catenin 2) is a Protein Coding gene. Among its related pathways are TGF-beta receptor signaling. GO annotations related to this gene include transcription factor binding and protein kinase C binding. An important paralog of this gene is DACT1. Involved in regulation of intracellular signaling pathways during development. Negatively regulates the Nodal signaling pathway, possibly by promoting the lysosomal degradation of Nodal receptors, such as TGFBR1. May be involved in control of the morphogenetic behavior of kidney ureteric bud cells by keeping cells epithelial and restraining their mesenchymal character. May play an inhibitory role in the re-epithelialization of skin wounds by attenuating TGF-beta signaling.
Background: DACT2 (Dishevelled Binding Antagonist Of Beta Catenin 2) is a Protein Coding gene. Among its related pathways are TGF-beta receptor signaling. GO annotations related to this gene include transcription factor binding and protein kinase C binding. An important paralog of this gene is DACT1. Involved in regulation of intracellular signaling pathways during development. Negatively regulates the Nodal signaling pathway, possibly by promoting the lysosomal degradation of Nodal receptors, such as TGFBR1. May be involved in control of the morphogenetic behavior of kidney ureteric bud cells by keeping cells epithelial and restraining their mesenchymal character. May play an inhibitory role in the re-epithelialization of skin wounds by attenuating TGF-beta signaling.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
dapper homolog 2
NCBI Official Synonym Full Names
dishevelled-binding antagonist of beta-catenin 2
NCBI Official Symbol
Dact2
NCBI Official Synonym Symbols
Dpr2; Frd2; dapper2; A630024E20; 2900084M21Rik
NCBI Protein Information
dapper homolog 2
UniProt Protein Name
Dapper homolog 2
Protein Family
UniProt Gene Name
Dact2
UniProt Synonym Gene Names
Dpr2; Frd2; mDpr2
UniProt Entry Name
DACT2_MOUSE
Research Articles on DACT2
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Product Notes
The Mouse DACT2 dact2 (Catalog #AAA9717947) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717947 ELISA Kit recognizes Mouse DACT2. It is sometimes possible for the material contained within the vial of "Dapper Homolog 2 (DACT2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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