Rabbit anti-Human CORO7 Polyclonal Antibody | anti-CORO7 antibody

CORO7, NT (CORO7, Coronin-7, 70kD WD repeat tumor rejection antigen homolog) (Azide free) (HRP)

Cat. Num. AAA6288047

• Gene Names: CORO7; CRN7; POD1; 0610011B16Rik
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CORO7; Polyclonal Antibody; NT (CORO7; Coronin-7; 70kD WD repeat tumor rejection antigen homolog) (Azide free) (HRP); 70kD WD repeat tumor rejection antigen homolog; anti-CORO7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Sequence Length: 925

• Applicable Applications for anti-CORO7 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: CORO7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 176-206 amino acids from the N-terminal region of human CORO7.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(CORO7 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the CORO7 antibody detected the CORO7 protein (arrow).)

Immunohistochemistry (IHC)

(CORO7 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CORO7 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-CORO7 antibody

Coronins, such as CORO7, constitute an evolutionarily conserved family of WD-repeat actin-binding proteins. CORO7 plays a role in Golgi complex morphology and function (Rybakin et al., 2004, 2006 [PubMed 15327992] [PubMed 16905771]).

Product Categories/Family for anti-CORO7 antibody

Antibodies; WD Repeat Proteins

References

Rose, J. Phd, et al. Mol. Med. (2010). In press: Rybakin, V., et al. Cell. Mol. Life Sci. 65(15):2419-2430(2008). Rybakin, V., et al. J. Biol. Chem. 281(41):31070-31078(2006). Rybakin, V., et al. FEBS Lett. 573 (1-3), 161-167 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 168229161
• NCBI GeneID: 79585
• NCBI Accession #: NP_078811.3
• NCBI GenBank Nucleotide #: NP_078811.3
• UniProt Accession #: P57737
• NCBI Official Full Name: coronin-7 isoform 1
• NCBI Official Synonym Full Names: coronin 7
• NCBI Official Symbol: CORO7
• NCBI Official Synonym Symbols: CRN7; POD1; 0610011B16Rik
• NCBI Protein Information: coronin-7
• UniProt Protein Name: Coronin-7
• Protein Family: Coronin
• UniProt Gene Name: CORO7
• UniProt Synonym Gene Names: Crn7
• UniProt Entry Name: CORO7_HUMAN

NCBI Description

This gene encodes a member of the coronin protein family. However, unlike other coronin proteins, it is not an actin-binding protein but rather functions as an F-actin regulator directing anterograde Golgi to endosome transport. The encoded protein has two tandem WD-40 domain repeats and localizes to the trans-Golgi network. The protein undergoes K33-linked polyubiquitination via an E3 ligase complex. It is thought to play an essential role in maintenance of Golgi apparatus morphology. Alternative splicing results in multiple transcripts variants; some of which form read-through transcripts with a neighboring gene. [provided by RefSeq, Dec 2016]

Uniprot Description

coronin 7: May play a role in the maintenance of the Golgi apparatus morphology and in the protein export from the Golgi. Belongs to the WD repeat coronin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: Golgi membrane; Golgi apparatus; membrane; cytoplasmic membrane-bound vesicle; integral to membrane; trans-Golgi network; cytosol; actin cytoskeleton

Molecular Function: actin filament binding; protein binding; actin binding

Biological Process: protein transport; actin filament polymerization; Golgi to endosome transport; actin cytoskeleton organization and biogenesis