Western Blot (WB)
(Sample Data: Western blot of rat brain lysate showing specific immunolabeling of the ~50kDa CAMK2A and the ~60kDa CAMK2B phosphorylated at Thr286 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-phospho-CAMK2A/2B (Thr286). The immunolabeling is completely eliminated by treatment with lambda-Ptase.)
Rabbit CAMK2A/2B Polyclonal Antibody | anti-phospho-CAMK2A/2B antibody
Anti-phospho-CAMK2A/2B (Thr286)
Reactivity
Human, Mouse, Rat, Xenopus
Applications
Western Blot
Purity
Affinity Chromatography
Synonyms
CAMK2A/2B; Polyclonal Antibody; Anti-phospho-CAMK2A/2B (Thr286); anti-phospho-CAMK2A/2B antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Xenopus
Clonality
Polyclonal
Isotype
Rabbit, IgG
Specificity
Recognizes the CAM2KA/2B protein phosphorylated at threonine 286
Purity/Purification
Affinity Chromatography
Form/Format
100 ul in 10mM HEPES (pH7.5), 150mMNaCl, 100 ug per ml BSA and 50% glycerol.
Applicable Applications for anti-phospho-CAMK2A/2B antibody
Western Blot (WB)
Application Notes
WB: 1:1,000
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr286 conjugated to KLH
Preparation and Storage
Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Western Blot (WB)
(Sample Data: Western blot of rat brain lysate showing specific immunolabeling of the ~50kDa CAMK2A and the ~60kDa CAMK2B phosphorylated at Thr286 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-phospho-CAMK2A/2B (Thr286). The immunolabeling is completely eliminated by treatment with lambda-Ptase.)
Western Blot (WB)
(Sample Data: Western blot of rat brain lysate showing specific immunolabeling of the ~50kDa CAMK2A and the ~60kDa CAMK2B phosphorylated at Thr286 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-phospho-CAMK2A/2B (Thr286). The immunolabeling is completely eliminated by treatment with lambda-Ptase.)
Related Product Information for anti-phospho-CAMK2A/2B antibody
Ca2+/calmodulin-dependent protein kinase II (CAMK2) is a multi-functional calcium and calmodulin-dependent serine/threonine kinase that mediates cellular responses to a wide variety of intercellular signals (1). CAMK2 is abundant in the brain and has been shown to regulate diverse cellular functions including synaptic plasticity, neurotransmitter synthesis and release, gene expression, ion channel function, carbohydrate metabolism, cytoskeletal function, and Ca2+-homeostasis (2,3). Phosphorylation of Thr286 produces an autonomously active form of CAMK2 (4). Autophosphorylation of Thr305 inhibits the activity CAMK2, which leads to reduced localization to the postsynaptic density and consequently reduced neuronal plasticity and capacity for learning (5).
Product Categories/Family for anti-phospho-CAMK2A/2B antibody
References
1. Kennedy, M B.: Signal transduction molecules at the glutamatergic postsynaptic membrane. 1998 Brain Res Rev 1998 26:243-257.
2. Gleason, M R. et al: Translocation of CaM kinase II to synaptic sites in vivo. Nature Neurosci 2003 6:217-218.
3. Hudmon, A. et al: Neuronal Ca2+/calmodulin-dependent protein kinase II: The role of structure and autoregulation in cellular function. Annu Rev Biochem 2002 71:473-510.
4. Meng, F J. et al: Autophosphorylated calcium/calmodulin-dependent protein kinase IIa (CaMKIIa) reversibly targets to and phosphorylates N-methyl-D-aspartate receptor subunit 2B (NR2B) in cerebral ischemia and reperfusion in hippocampus of rats. Brain Res 2003 967:161-169.
5. Elgersma, Y. et al: Inhibitory autophosphorylation of CaMKII controls PSD association, plasticity, and learning. Neuron 200236:493-505.
2. Gleason, M R. et al: Translocation of CaM kinase II to synaptic sites in vivo. Nature Neurosci 2003 6:217-218.
3. Hudmon, A. et al: Neuronal Ca2+/calmodulin-dependent protein kinase II: The role of structure and autoregulation in cellular function. Annu Rev Biochem 2002 71:473-510.
4. Meng, F J. et al: Autophosphorylated calcium/calmodulin-dependent protein kinase IIa (CaMKIIa) reversibly targets to and phosphorylates N-methyl-D-aspartate receptor subunit 2B (NR2B) in cerebral ischemia and reperfusion in hippocampus of rats. Brain Res 2003 967:161-169.
5. Elgersma, Y. et al: Inhibitory autophosphorylation of CaMKII controls PSD association, plasticity, and learning. Neuron 200236:493-505.
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Product Notes
The phospho-CAMK2A/2B (Catalog #AAA515889) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-phospho-CAMK2A/2B (Thr286) reacts with Human, Mouse, Rat, Xenopus and may cross-react with other species as described in the data sheet. AAA Biotech's CAMK2A/2B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1,000. Researchers should empirically determine the suitability of the phospho-CAMK2A/2B for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CAMK2A/2B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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