Western Blot (WB)
(Western blot analysis of lysate of human CD14+ peripheral blood mononuclear cells (PBMC). PVDF Membrane was probed with 1ug/ml of MBS647676 followed by HRP-conjugated sheep IgG secondary antibody. A specific band was detected for CLEC9a at approximately 45kD. This experiment was conducted under reducing conditions. The following buffers were used: blotting buffer: 25mM Tris, pH 7.4, 0.5M NaCl and 0.05% Tween-20; blocking solution: 3% BSA in blotting buffer, pH adjusted to 7.4; antibody solution: 2% dry non-fat dry milk in blotting buffer, pH adjusted to 7.4.)
Sheep anti-Human CLEC9a Polyclonal Antibody | anti-CLEC9A antibody
CLEC9a (C-type Lectin Domain Family 9 Member A, UNQ9341/PRO34046)
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Dilution: Western Blot: 1ug/ml
Immunohistochemistry: 5-15ug/ml
Western Blot (WB)
(Western blot analysis of lysate of human CD14+ peripheral blood mononuclear cells (PBMC). PVDF Membrane was probed with 1ug/ml of MBS647676 followed by HRP-conjugated sheep IgG secondary antibody. A specific band was detected for CLEC9a at approximately 45kD. This experiment was conducted under reducing conditions. The following buffers were used: blotting buffer: 25mM Tris, pH 7.4, 0.5M NaCl and 0.05% Tween-20; blocking solution: 3% BSA in blotting buffer, pH adjusted to 7.4; antibody solution: 2% dry non-fat dry milk in blotting buffer, pH adjusted to 7.4.)
Western Blot (WB)
(Western blot analysis of lysate of human CD14+ peripheral blood mononuclear cells (PBMC). PVDF Membrane was probed with 1ug/ml of MBS647676 followed by HRP-conjugated sheep IgG secondary antibody. A specific band was detected for CLEC9a at approximately 45kD. This experiment was conducted under reducing conditions. The following buffers were used: blotting buffer: 25mM Tris, pH 7.4, 0.5M NaCl and 0.05% Tween-20; blocking solution: 3% BSA in blotting buffer, pH adjusted to 7.4; antibody solution: 2% dry non-fat dry milk in blotting buffer, pH adjusted to 7.4.)
Immunohistochemistry (IHC)
(IHC staining of immersion fixed, paraffin-embedded sections of human spleen using MBS647676 at 15ug/ml overnight at 4°C. Tissue was stained using the anti-sheep HRP-DAB Cell & Tissue Staining Kit and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm.)
Immunohistochemistry (IHC)
(IHC staining of immersion fixed, paraffin-embedded sections of human spleen using MBS647676 at 15ug/ml overnight at 4°C. Tissue was stained using the anti-sheep HRP-DAB Cell & Tissue Staining Kit and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm.)
NCBI and Uniprot Product Information
NCBI Description
CLEC9A is a group V C-type lectin-like receptor (CTLR) that functions as an activation receptor and is expressed on myeloid lineage cells (Huysamen et al., 2008 [PubMed 18408006]).[supplied by OMIM, Aug 2008]
Uniprot Description
Function: Functions as an endocytic receptor on a small subset of myeloid cells specialized for the uptake and processing of material from dead cells. Recognizes filamentous form of actin in association with particular actin-binding domains of cytoskeletal proteins, including spectrin, exposed when cell membranes are damaged, and mediate the cross-presentation of dead-cell associated antigens in a Syk-dependent manner. Ref.3 Ref.4
Subunit structure: Homodimer. Ref.1 Ref.4
Subcellular location: Membrane; Single-pass type II membrane protein Ref.1.
Tissue specificity: In peripheral blood highly restricted on the surface of BDCA31+ dendritic cells and on a small subset of CD14+ and CD16- monocytes. Ref.1 Ref.3
Post-translational modification: N-glycosylated. Ref.1
Sequence similarities: Contains 1 C-type lectin domain.
Research Articles on CLEC9A
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Product Notes
The CLEC9A clec9a (Catalog #AAA647676) is an Antibody produced from Sheep and is intended for research purposes only. The product is available for immediate purchase. The CLEC9a (C-type Lectin Domain Family 9 Member A, UNQ9341/PRO34046) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CLEC9a can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Western Blot and Immunohistochemistry. Dilution: Western Blot: 1ug/ml Immunohistochemistry: 5-15ug/ml. Researchers should empirically determine the suitability of the CLEC9A clec9a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CLEC9a, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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