Western Blot (WB)
(Detection of Human Cadherin-15 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 1ug/ml of MBS624331 followed by HRPconjugated Anti-Sheep IgG Secondary Antibody. A specific band was detected for Cadherin-15 at ~130kD (as indicated). This experiment was conducted under reducing conditions.)
Sheep anti-Human, Mouse Cadherin-15 Polyclonal Antibody | anti-CDH15 antibody
Cadherin-15 (Cadherin, M, Muscle Cadherin, M-cadherin, Cadherin-14, CDH15, CDH14, CDH3)
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Dilution: Flow Cytometry: 2.5ug labels 10e6 cells from the C2C12 mouse myoblast cell line.
Western Blot: 1ug/ml detects Caderin-15 in HeLa cells.
Immunocytochemistry: 5-15ug/ml detects Cadherin-15 in immersion fixed C2C12 mouse cells.
Western Blot (WB)
(Detection of Human Cadherin-15 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 1ug/ml of MBS624331 followed by HRPconjugated Anti-Sheep IgG Secondary Antibody. A specific band was detected for Cadherin-15 at ~130kD (as indicated). This experiment was conducted under reducing conditions.)
Western Blot (WB)
(Detection of Human Cadherin-15 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 1ug/ml of MBS624331 followed by HRPconjugated Anti-Sheep IgG Secondary Antibody. A specific band was detected for Cadherin-15 at ~130kD (as indicated). This experiment was conducted under reducing conditions.)
Flow Cytometry (FC/FACS)
(Detection of Cadherin-15 in C2C12 mouse cell line by Flow Cytometry. C2C12 mouse myoblast cell line was stained with MBS624331 (filled histogram) or control antibody (open histogram), followed by NorthernLights™ 557conjugated Anti-Sheep IgG Secondary Antibody.)
Flow Cytometry (FC/FACS)
(Detection of Cadherin-15 in C2C12 mouse cell line by Flow Cytometry. C2C12 mouse myoblast cell line was stained with MBS624331 (filled histogram) or control antibody (open histogram), followed by NorthernLights™ 557conjugated Anti-Sheep IgG Secondary Antibody.)
Testing Data
(Cadherin-15 in C2C12 mouse cell line. Cadherin-15 was detected in immersion fixed C2C12 mouse myoblast cell line using MBS624331 at 10ug/ml for 3 hours at RT. Cells were stained using the Northern Lights™ 557c-onjugated Anti-Mouse IgG Secondary Antibody (red) a n d counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm.)
Testing Data
(Cadherin-15 in C2C12 mouse cell line. Cadherin-15 was detected in immersion fixed C2C12 mouse myoblast cell line using MBS624331 at 10ug/ml for 3 hours at RT. Cells were stained using the Northern Lights™ 557c-onjugated Anti-Mouse IgG Secondary Antibody (red) a n d counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm.)
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of the cadherin superfamily of genes, encoding calcium-dependent intercellular adhesion glycoproteins. Cadherins consist of an extracellular domain containing 5 cadherin domains, a transmembrane region, and a conserved cytoplasmic domain. Transcripts from this particular cadherin are expressed in myoblasts and upregulated in myotubule-forming cells. The protein is thought to be essential for the control of morphogenetic processes, specifically myogenesis, and may provide a trigger for terminal muscle cell differentiation. [provided by RefSeq]
Uniprot Description
CDH15: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. M-cadherin is part of the myogenic program and may provide a trigger for terminal muscle differentiation. A chromosomal aberration involving CDH15 and KIRREL3 is found in a patient with severe mental retardation and dysmorphic facial features. Translocation t(11;16)(q24.2;q24). Defects in CDH15 are the cause of mental retardation autosomal dominant type 3 (MRD3). Mental retardation is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 16q24.3
Cellular Component: integral to membrane; plasma membrane; caveola; neuromuscular junction
Molecular Function: calcium ion binding
Biological Process: intercellular junction assembly and maintenance; muscle cell differentiation; positive regulation of muscle cell differentiation; homophilic cell adhesion; cell adhesion
Disease: Mental Retardation, Autosomal Dominant 3
Research Articles on CDH15
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Product Notes
The CDH15 cdh15 (Catalog #AAA624331) is an Antibody produced from Sheep and is intended for research purposes only. The product is available for immediate purchase. The Cadherin-15 (Cadherin, M, Muscle Cadherin, M-cadherin, Cadherin-14, CDH15, CDH14, CDH3) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Cadherin-15 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Suitable for use in ELISA, Flow Cytometry, Western Blot and Immunocytochemistry. Dilution: Flow Cytometry: 2.5ug labels 10e6 cells from the C2C12 mouse myoblast cell line. Western Blot: 1ug/ml detects Caderin-15 in HeLa cells. Immunocytochemistry: 5-15ug/ml detects Cadherin-15 in immersion fixed C2C12 mouse cells. Researchers should empirically determine the suitability of the CDH15 cdh15 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cadherin-15, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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