Western Blot (WB)
(AP-2 pAb tested by Western blot. Detection of AP-2 by Western blot analysis. Activator protein 2 (AP-2) can be detected in JEG-3 nuclear extract using AP-2 rabbit polyclonal antibody at a 1:1,000 dilution.)
Rabbit anti-Human AP-2 Polyclonal Antibody | anti-AP-2 antibody
AP-2 antibody (pAb)
Gene Names
TFAP2A; AP-2; BOFS; AP2TF; TFAP2; AP-2alpha
Reactivity
Human
Applications
Chromatin Immunoprecipitation, Immunoprecipitation, Immunohistochemistry, Gel Super Shift Assay, Western Blot
Purity
Affinity Purified
Synonyms
AP-2; Polyclonal Antibody; AP-2 antibody (pAb); TFAP2A; BOFS; AP2TF; TFAP2; AP-2alpha; anti-AP-2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
PBS containing 0.2% gelatin, 0.01% thimerosal and 0.1% sodium azide. Thimerosal and sodium azide are highly toxic.
Applicable Applications for anti-AP-2 antibody
Chromatin Immunoprecipitation (ChIP), Immunohistochemistry (IHC), Electrophoretic Mobility Shift Assay (EMSA), Western Blot (WB)
Application Notes
Applications Validated:
WB: 1:500-1:1,000
For optimal results, primary antibody incubations should be performed overnight at 4 degree C. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background.
WB: 1:500-1:1,000
For optimal results, primary antibody incubations should be performed overnight at 4 degree C. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background.
Immunogen
This AP-2 antibody was raised against a synthetic peptide corresponding to amino acid residues 120-134 of human AP-2.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Western Blot (WB)
(AP-2 pAb tested by Western blot. Detection of AP-2 by Western blot analysis. Activator protein 2 (AP-2) can be detected in JEG-3 nuclear extract using AP-2 rabbit polyclonal antibody at a 1:1,000 dilution.)
Western Blot (WB)
(AP-2 pAb tested by Western blot. Detection of AP-2 by Western blot analysis. Activator protein 2 (AP-2) can be detected in JEG-3 nuclear extract using AP-2 rabbit polyclonal antibody at a 1:1,000 dilution.)
Related Product Information for anti-AP-2 antibody
Short Description: Our AP-2 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, Immunohistochemistry, Supershift and Western blot; it has been shown to react with Human samples.
Background: AP-2 (activator protein 2 or AP2) transcription factors activate transcription and regulate gene expression during embryogenesis. There are four different isoforms of AP-2 (AP-2a, AP-2b, AP-2g and AP-2d). The family members share a common structure in which the N-terminal region is involved with transcriptional activation and the C-terminal region is involved in DNA binding.
Background: AP-2 (activator protein 2 or AP2) transcription factors activate transcription and regulate gene expression during embryogenesis. There are four different isoforms of AP-2 (AP-2a, AP-2b, AP-2g and AP-2d). The family members share a common structure in which the N-terminal region is involved with transcriptional activation and the C-terminal region is involved in DNA binding.
Product Categories/Family for anti-AP-2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
47,440 Da
NCBI Official Full Name
transcription factor AP-2-alpha isoform b
NCBI Official Synonym Full Names
transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha)
NCBI Official Symbol
TFAP2A
NCBI Official Synonym Symbols
AP-2; BOFS; AP2TF; TFAP2; AP-2alpha
NCBI Protein Information
transcription factor AP-2-alpha; AP-2 transcription factor; AP2-alpha; activating enhancer-binding protein 2-alpha; activator protein 2
UniProt Protein Name
Transcription factor AP-2-alpha
Protein Family
UniProt Gene Name
TFAP2A
UniProt Synonym Gene Names
AP2TF; TFAP2; AP2-alpha; AP-2
UniProt Entry Name
AP2A_HUMAN
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Product Notes
The AP-2 tfap2a (Catalog #AAA388324) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AP-2 antibody (pAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's AP-2 can be used in a range of immunoassay formats including, but not limited to, Chromatin Immunoprecipitation (ChIP), Immunohistochemistry (IHC), Electrophoretic Mobility Shift Assay (EMSA), Western Blot (WB). Applications Validated: WB: 1:500-1:1,000 For optimal results, primary antibody incubations should be performed overnight at 4 degree C. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background. Researchers should empirically determine the suitability of the AP-2 tfap2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AP-2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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