Western Blot (WB)
(Western blot analysis of extracts of mouse eye, using TFAP2A antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 15s.)
Rabbit AP-2alpha Polyclonal Antibody | anti-TFAP2A antibody
AP-2alpha Rabbit pab Antibody
Gene Names
TFAP2A; AP-2; BOFS; AP2TF; TFAP2; AP-2alpha
Reactivity
Human, mouse, rat
Applications
Western Blot
Purity
Affinity purification
Synonyms
AP-2alpha; Polyclonal Antibody; AP-2alpha Rabbit pab Antibody; TFAP2A; AP-2alpha ; AP-2a ; AP-2; AP2TF; BOFS; FLJ51761; TFAP2 ; AP2; anti-TFAP2A antibody
Host
Rabbit
Reactivity
Human, mouse, rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
Liquid
Sequence Length
431
Applicable Applications for anti-TFAP2A antibody
Western Blot (WB)
Application Notes
Western Blot (WB): 1/500-1000
Immunogen
A fusion protein of human AP-2alpha
Buffer
PBS with 0.1% Sodium Azide, 50% Glycerol,
Preparation and Storage
Upon delivery aliquot and store at -20 degree C or -80 degree C.
Western Blot (WB)
(Western blot analysis of extracts of mouse eye, using TFAP2A antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 15s.)
Western Blot (WB)
(Western blot analysis of extracts of mouse eye, using TFAP2A antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 15s.)
Related Product Information for anti-TFAP2A antibody
Rabbit polyclonal to Human AP-2alpha
The sequence-specific transcription factor activator protein 2alpha (AP-2alpha) is required for normal growth and morphogenesis during mammalian development (1,2). Decreased or loss of AP-2alpha expression has been observed in many different types of human cancers including breast cancer (3,4), ovarian cancer (5), melanoma (6) and prostate cancer (7). These findings suggest that AP-2alpha expression plays a crucial role in tumorigenicity. Studies have also shown that p53 overexpression in human breast carcinoma cells induces the level of AP-2alpha expression. Furthermore, p53 binds to the cis-element in the AP-2alpha promoter, suggesting that AP-2alpha is a target of p53. AP-2alpha may mediate the effect of p53 to inhibit cell proliferation (8).
The sequence-specific transcription factor activator protein 2alpha (AP-2alpha) is required for normal growth and morphogenesis during mammalian development (1,2). Decreased or loss of AP-2alpha expression has been observed in many different types of human cancers including breast cancer (3,4), ovarian cancer (5), melanoma (6) and prostate cancer (7). These findings suggest that AP-2alpha expression plays a crucial role in tumorigenicity. Studies have also shown that p53 overexpression in human breast carcinoma cells induces the level of AP-2alpha expression. Furthermore, p53 binds to the cis-element in the AP-2alpha promoter, suggesting that AP-2alpha is a target of p53. AP-2alpha may mediate the effect of p53 to inhibit cell proliferation (8).
References
1. Mitchell, P.J. et al. (1987) Cell 50, 847-61. 2. Williams, T. and Tjian, R. (1991) Genes Dev 5, 670-82. 3. Douglas, D.B. et al. (2004) Cancer Res 64, 1611-20. 4. Gee, J.M. et al. (1999) J Pathol 189, 514-20. 5. Anttila, M.A. et al. (2000) Br J Cancer 82, 1974-83. 6. Jean, D. et al. (1998) J Biol Chem 273, 16501-8. 7. Ruiz, M. et al. (2004) Cancer Res 64, 631-8. 8. Li, H. et al. (2006) Oncogene 25, 5405-15.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
437
NCBI Official Full Name
transcription factor AP-2-alpha isoform b
NCBI Official Synonym Full Names
transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha)
NCBI Official Symbol
TFAP2A
NCBI Official Synonym Symbols
AP-2; BOFS; AP2TF; TFAP2; AP-2alpha
NCBI Protein Information
transcription factor AP-2-alpha; AP2-alpha; activator protein 2; AP-2 transcription factor; activating enhancer-binding protein 2-alpha
UniProt Protein Name
Transcription factor AP-2-alpha
Protein Family
UniProt Gene Name
TFAP2A
UniProt Synonym Gene Names
AP2TF; TFAP2; AP2-alpha; AP-2
UniProt Entry Name
AP2A_HUMAN
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Product Notes
The TFAP2A tfap2a (Catalog #AAA125382) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AP-2alpha Rabbit pab Antibody reacts with Human, mouse, rat and may cross-react with other species as described in the data sheet. AAA Biotech's AP-2alpha can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot (WB): 1/500-1000. Researchers should empirically determine the suitability of the TFAP2A tfap2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AP-2alpha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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