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Western Blot (WB)
(Western blot analysis of AP2 alpha on human AP2 alpha recombinant protein using anti- AP2 alpha antibody at 1/1, 000 dilution.)
Mouse anti-Human TFAP2A Monoclonal Antibody | anti-TFAP2A antibody
TFAP2A Antibody
Gene Names
TFAP2A; AP-2; BOFS; AP2TF; TFAP2; AP-2alpha
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
TFAP2A; Monoclonal Antibody; TFAP2A Antibody; Activating enhancer binding protein 2 alpha antibody; Activating enhancer-binding protein 2-alpha antibody; Activator protein 2 antibody; AP 2 transcription factor antibody; AP 2alpha antibody; AP-2 antibody; AP-2 transcription factor antibody; AP2 antibody; AP2 Transcription Factor antibody; AP2-alpha antibody; AP2A_HUMAN antibody; AP2TF antibody; BOFS antibody; FLJ51761 antibody; TFAP 2 antibody; TFAP 2A antibody; TFAP2 antibody; TFAP2A antibody; Transcription factor AP 2 alpha (activating enhancer binding protein 2 alpha) antibody; Transcription factor AP-2-alpha antibody; Transcription factor AP2 alpha antibody; anti-TFAP2A antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Clone Number
10-C5
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-TFAP2A antibody
Western Blot (WB), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500-1:2000
ICC: 1:50-1:200
FC/FACS: 1:50-1:100
ICC: 1:50-1:200
FC/FACS: 1:50-1:100
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
Western Blot (WB)
(Western blot analysis of AP2 alpha on human AP2 alpha recombinant protein using anti- AP2 alpha antibody at 1/1, 000 dilution.)
Western Blot (WB)
(Western blot analysis of AP2 alpha on human AP2 alpha recombinant protein using anti- AP2 alpha antibody at 1/1, 000 dilution.)
Western Blot (WB)
(Western blot analysis of AP2 alpha on HEK293 (1) and AP2 alpha -hIgGFc transfected HEK293 (2) cell lysate using anti- AP2 alpha antibody at 1/1, 000 dilution.)
Western Blot (WB)
(Western blot analysis of AP2 alpha on HEK293 (1) and AP2 alpha -hIgGFc transfected HEK293 (2) cell lysate using anti- AP2 alpha antibody at 1/1, 000 dilution.)
Immunocytochemistry (ICC)
(ICC staining AP2 alpha (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining AP2 alpha (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Hela cells with AP2 alpha antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Hela cells with AP2 alpha antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).)
Related Product Information for anti-TFAP2A antibody
AP-2 transcription factor family members include AP-2alpha, AP-2beta and AP-2gamma, which specifically bind to the DNA consensus sequence CCCCAGGC and initiate transcription of selected genes. AP-2, also known as ERF-1, plays a role in regulating estrogen receptor expression. AP-2beta , a splice variant of AP-2alpha, inhibits AP-2 activity. Besides subscribing to the AP-2 complex, AP-2alpha, AP-2beta and AP-2gamma proteins compose the OB2-1 transcription factor complex. OB2-1 specifically upregulates expression of the proto-oncogene c-ErbB-2, which is overexpressed in 25-30% of breast cancers. AP-2alpha may play an important role in the development of ectodermal-derived tissues. Deleterious mutations involving the AP-2alpha gene are linked to microphthalmia, corneal clouding and other anterior eye chamber defects. The ubiquitously expressed AP-4 transcription factor specifically binds to the DNA consensus sequence 5'-CAGCTG-3'. AP-4 interacts with promoters for immunoglobulin-kappa gene families and simian virus 40. AP-4 may enhance the transcription of the human Huntington's disease gene. AP-4 is a helix-loop-helix protein that contains two distinctive leucine repeat elements.
Product Categories/Family for anti-TFAP2A antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
437
NCBI Official Full Name
transcription factor AP-2-alpha isoform b
NCBI Official Synonym Full Names
transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha)
NCBI Official Symbol
TFAP2A
NCBI Official Synonym Symbols
AP-2; BOFS; AP2TF; TFAP2; AP-2alpha
NCBI Protein Information
transcription factor AP-2-alpha; AP2-alpha; activator protein 2; AP-2 transcription factor; activating enhancer-binding protein 2-alpha
UniProt Protein Name
Transcription factor AP-2-alpha
Protein Family
UniProt Gene Name
TFAP2A
UniProt Synonym Gene Names
AP2TF; TFAP2; AP2-alpha; AP-2
UniProt Entry Name
AP2A_HUMAN
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Product Notes
The TFAP2A tfap2a (Catalog #AAA9435244) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The TFAP2A Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TFAP2A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS). WB: 1:500-1:2000 ICC: 1:50-1:200 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the TFAP2A tfap2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TFAP2A, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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