Goat TFAP2A / AP-2 Polyclonal Antibody | anti-TFAP2A antibody

Goat Polyclonal to Human TFAP2A / AP-2

Cat. Num. AAA240508

• Gene Names: TFAP2A; AP-2; BOFS; AP2TF; TFAP2; AP-2alpha
• Reactivity: Chimpanzee, Chicken, Dog, Human, Mouse, Orangutan, Rat
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: TFAP2A / AP-2; Polyclonal Antibody; Goat Polyclonal to Human TFAP2A / AP-2; Anti-TFAP2A / AP-2 Antibody (aa3-14) IHC-plus; TFAP2A; Activator protein 2; AP-2; AP-2a; AP2TF; AP-2alpha; BOFS; TFAP2; Transcription factor AP-2; AP-2 transcription factor; AP2-alpha; Human TFAP2A; anti-TFAP2A antibody

• Host: Goat
• Reactivity: Chimpanzee, Chicken, Dog, Human, Mouse, Orangutan, Rat
• Clonality: Polyclonal
• Specificity: aa 3-14 of Human AP2A1 and AP2A2 proteins.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
• Sequence Length: 437

• Applicable Applications for anti-TFAP2A antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:5000 - 1:20000), IF, IHC-P (2.5 ug/ml), WB (1:500 - 1:2000); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...
• Target Species: Human
• Immunogen Description: Synthetic peptide from human TFAP2A / AP2 Alpha.
• Immunogen Type: Synthetic peptide
• Immunogen: TFAP2A / AP-2 antibody was raised against synthetic peptide from human TFAP2A / AP2 Alpha.
• Antigen Modification: aa3-14
• Preparation and Storage: +4 degree C or -20 degree C, Avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Anti-TFAP2A / AP2 Alpha antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Anti-AP2A Antibody - Western Blot. Western blot of Affinity Purified anti-AP2A antibody shows detection of a band just below 100 kD corresponding to Human AP2A1 in a various preparations. Lane 1 - HeLa nuclear extract, Lane 2 - HeLa, Lane 3 - 293, Lane 4 - A431 and Lane 5 - Jurkat whole cell lysates. In lanes 6-10 the antibody was preincubated with 1 ug/ml of the immunizing peptide which effectively blocks the specific reactivity of this antibody with AP2A. Approximately 20 ug of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-AP2A antibody. Detection occurred using a 1:5000 dilution of HRP-labeled Rabbit anti-Goat IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 60-sec exposure time.)

Related Product Information for anti-TFAP2A antibody

Caution: AP-2B refers to both an alternatively spliced form of AP-2 and to the related transcription factor AP-2 beta.

NCBI and Uniprot Product Information

• NCBI GI #: 4507441
• NCBI GeneID: 7020
• NCBI Accession #: NP_003211.1
• NCBI GenBank Nucleotide #: NM_003220.2
• UniProt Accession #: P05549; Q13777; Q5TAV5; Q8N1C6
• Molecular Weight: 47,440 Da
• NCBI Official Full Name: transcription factor AP-2-alpha isoform a
• NCBI Official Synonym Full Names: transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha)
• NCBI Official Symbol: TFAP2A
• NCBI Official Synonym Symbols: AP-2; BOFS; AP2TF; TFAP2; AP-2alpha
• NCBI Protein Information: transcription factor AP-2-alpha; AP2-alpha; activator protein 2; AP-2 transcription factor; activating enhancer-binding protein 2-alpha
• UniProt Protein Name: Transcription factor AP-2-alpha
• Protein Family: Transcription factor
• UniProt Gene Name: TFAP2A
• UniProt Synonym Gene Names: AP2TF; TFAP2; AP2-alpha; AP-2
• UniProt Entry Name: AP2A_HUMAN

NCBI Description

The protein encoded by this gene is a transcription factor that binds the consensus sequence 5'-GCCNNNGGC-3'. The encoded protein functions as either a homodimer or as a heterodimer with similar family members. This protein activates the transcription of some genes while inhibiting the transcription of others. Defects in this gene are a cause of branchiooculofacial syndrome (BOFS). Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Dec 2009]

Uniprot Description

AP-2 alpha: Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-alpha is the only AP-2 protein required for early morphogenesis of the lens vesicle. Together with the CITED2 coactivator, stimulates the PITX2 P1 promoter transcription activation. Associates with chromatin to the PITX2 P1 promoter region. Binds DNA as a dimer. Can form homodimers or heterodimers with other AP-2 family members. Interacts with WWOX. Interacts with CITED4. Interacts with UBE2I. Interacts with RALBP1 in a complex also containing EPN1 and NUMB during interphase and mitosis. Interacts with KCTD1; this interaction represses transcription activation. Interacts (via C-terminus) with CITED2 (via C-terminus); the interaction stimulates TFAP2A- transcriptional activation. Interacts (via N-terminus) with EP300 (via N-terminus); the interaction requires CITED2. Belongs to the AP-2 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 6p24

Cellular Component: nucleoplasm; Golgi apparatus; centrosome; intracellular membrane-bound organelle; cytoplasm; nucleus

Molecular Function: protein dimerization activity; protein binding; protein homodimerization activity; sequence-specific DNA binding; transcription coactivator activity; chromatin binding

Biological Process: embryonic forelimb morphogenesis; negative regulation of epidermal growth factor receptor signaling pathway; transcription from RNA polymerase II promoter; neural crest cell development; regulation of neuron differentiation; positive regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; palate development; embryonic pattern specification; negative regulation of cell proliferation; sympathetic nervous system development; sensory perception of sound; oculomotor nerve formation; epidermis morphogenesis; positive regulation of neuron apoptosis; regulation of cell differentiation; negative regulation of neuron apoptosis; kidney development; inner ear morphogenesis; trigeminal nerve development; anterior neuropore closure; embryonic cranial skeleton morphogenesis; positive regulation of bone mineralization; embryonic body morphogenesis; forebrain neuron development; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of cell migration; negative regulation of apoptosis

Disease: Branchiooculofacial Syndrome