Rabbit anti-Human, Rat AP2 alpha Polyclonal Antibody | anti-TFAP2A antibody

Anti-AP2 alpha Antibody

Cat. Num. AAA176871

• Gene Names: TFAP2A; AP-2; BOFS; AP2TF; TFAP2; AP-2alpha
• Reactivity: Human, Rat
• Applications: Western Blot
• Purity: Immunogen affinity purified.
• Synonyms: AP2 alpha; Polyclonal Antibody; Anti-AP2 alpha Antibody; Transcription factor AP-2-alpha; transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha); Activating enhancer binding protein 2 alpha antibody; Activating enhancer-binding protein 2-alpha antibody; Activator protein 2 antibody; AP 2 transcription factor antibody; AP 2alpha antibody; AP-2 antibody; AP-2 transcription factor antibody; AP2 antibody; AP2 Transcription Factor antibody; AP2-alpha antibody; AP2A_HUMAN antibody; AP2TF antibody; BOFS antibody; FLJ51761 antibody; TFAP 2 antibody; TFAP 2A antibody; TFAP2 antibody; TFAP2A antibody; Transcription factor AP 2 alpha (activating enhancer binding protein 2 alpha) antibody; Transcription factor AP-2-alpha antibody; Transcription factor AP2 alpha antibody; anti-TFAP2A antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 437

• Applicable Applications for anti-TFAP2A antibody: Western Blot (WB)
• Immunogen: E Coli-derived human AP2 alpha recombinant protein (Position: M1-G166). Human AP2 alpha shares 98% amino acid (aa) sequence identity with both mouse and rat AP2 alpha.
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Testing Data

(Anti-AP2 alpha Picoband antibody, MBS176871-1.jpgAll lanes: Anti AP2A (MBS176871) at 0.5ug/mlWB: Recombinant Human AP2A Protein 0.5ngPredicted bind size: 38KDObserved bind size: 38KD )

Western Blot (WB)

(Anti-AP2 alpha Picoband antibody, MBS176871-2.jpgAll lanes: Anti AP2A (MBS176871) at 0.5ug/mlWB: Rat Spleen Tissue Lysate at 50ugPredicted bind size: 48KDObserved bind size: 48KD )

Related Product Information for anti-TFAP2A antibody

Description: Rabbit IgG polyclonal antibody for Transcription factor AP-2-alpha(TFAP2A) detection. Tested with WB in Human, Rat.
Background: TFAP2A, also known as AP-2alpha or BOFS, is a protein that in humans is encoded by the TFAP2A gene. It is mapped to 6p24.3. The protein encoded by this gene is a transcription factor that binds the consensus sequence 5'-GCCNNNGGC-3'. The encoded protein functions as either a homodimer or as a heterodimer with similar family members. This protein activates the transcription of some genes while inhibiting the transcription of others. TFAP2A acts as a sequence-specific DNA-binding transcription factor recognizing and binding to the specific DNA sequence and recruiting transcription machinery. This gene is expressed in neural crest cell lineages with the highest levels of expression corresponding to early neural crest cells, suggesting that TFAP2A plays a role in their differentiation and development.

NCBI and Uniprot Product Information

• NCBI GI #: 135302
• NCBI GeneID: 7020
• UniProt Accession #: P05549; Q13777; Q5TAV5; Q8N1C6
• Molecular Weight: 47,440 Da
• NCBI Official Full Name: Transcription factor AP-2-alpha
• NCBI Official Synonym Full Names: transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha)
• NCBI Official Symbol: TFAP2A
• NCBI Official Synonym Symbols: AP-2; BOFS; AP2TF; TFAP2; AP-2alpha
• NCBI Protein Information: transcription factor AP-2-alpha; AP2-alpha; activator protein 2; AP-2 transcription factor; activating enhancer-binding protein 2-alpha
• UniProt Protein Name: Transcription factor AP-2-alpha
• Protein Family: Transcription factor
• UniProt Gene Name: TFAP2A
• UniProt Synonym Gene Names: AP2TF; TFAP2; AP2-alpha; AP-2
• UniProt Entry Name: AP2A_HUMAN

NCBI Description

The protein encoded by this gene is a transcription factor that binds the consensus sequence 5'-GCCNNNGGC-3'. The encoded protein functions as either a homodimer or as a heterodimer with similar family members. This protein activates the transcription of some genes while inhibiting the transcription of others. Defects in this gene are a cause of branchiooculofacial syndrome (BOFS). Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Dec 2009]

Uniprot Description

AP-2 alpha: Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-alpha is the only AP-2 protein required for early morphogenesis of the lens vesicle. Together with the CITED2 coactivator, stimulates the PITX2 P1 promoter transcription activation. Associates with chromatin to the PITX2 P1 promoter region. Binds DNA as a dimer. Can form homodimers or heterodimers with other AP-2 family members. Interacts with WWOX. Interacts with CITED4. Interacts with UBE2I. Interacts with RALBP1 in a complex also containing EPN1 and NUMB during interphase and mitosis. Interacts with KCTD1; this interaction represses transcription activation. Interacts (via C-terminus) with CITED2 (via C-terminus); the interaction stimulates TFAP2A- transcriptional activation. Interacts (via N-terminus) with EP300 (via N-terminus); the interaction requires CITED2. Belongs to the AP-2 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 6p24

Cellular Component: nucleoplasm; Golgi apparatus; centrosome; intracellular membrane-bound organelle; cytoplasm; nucleus

Molecular Function: protein dimerization activity; protein binding; protein homodimerization activity; sequence-specific DNA binding; transcription coactivator activity; chromatin binding

Biological Process: negative regulation of epidermal growth factor receptor signaling pathway; transcription from RNA polymerase II promoter; embryonic forelimb morphogenesis; neural crest cell development; regulation of neuron differentiation; positive regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; palate development; embryonic pattern specification; negative regulation of cell proliferation; sympathetic nervous system development; sensory perception of sound; oculomotor nerve formation; regulation of cell differentiation; epidermis morphogenesis; positive regulation of neuron apoptosis; negative regulation of neuron apoptosis; kidney development; inner ear morphogenesis; trigeminal nerve development; anterior neuropore closure; embryonic cranial skeleton morphogenesis; positive regulation of bone mineralization; embryonic body morphogenesis; forebrain neuron development; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis; positive regulation of cell migration

Disease: Branchiooculofacial Syndrome