Western Blot (WB)
(Western blot detection of AP-2alpha in A549, MCF7, C6 and Hela cell lysates using AP-2alpha mouse mAb (1:1000 diluted).Predicted band size:48KDa.Observed band size:48KDa.)
Mouse anti-Human, Rat AP-2alpha Monoclonal Antibody | anti-AP-2alpha antibody
Anti-AP-2alpha Mouse mAb
Western Blot (WB)
(Western blot detection of AP-2alpha in A549, MCF7, C6 and Hela cell lysates using AP-2alpha mouse mAb (1:1000 diluted).Predicted band size:48KDa.Observed band size:48KDa.)
Western Blot (WB)
(Western blot detection of AP-2alpha in A549, MCF7, C6 and Hela cell lysates using AP-2alpha mouse mAb (1:1000 diluted).Predicted band size:48KDa.Observed band size:48KDa.)
UniPort Summary Function: Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5-GCCNNNGGC-3 and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-alpha is the only AP-2 protein required for early morphogenesis of the lens vesicle. Together with the CITED2 coactivator, stimulates the PITX2 P1 promoter transcription activation. Associates with chromatin to the PITX2 P1 promoter region.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a transcription factor that binds the consensus sequence 5'-GCCNNNGGC-3'. The encoded protein functions as either a homodimer or as a heterodimer with similar family members. This protein activates the transcription of some genes while inhibiting the transcription of others. Defects in this gene are a cause of branchiooculofacial syndrome (BOFS). Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Dec 2009]
Uniprot Description
AP-2 alpha: Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-alpha is the only AP-2 protein required for early morphogenesis of the lens vesicle. Together with the CITED2 coactivator, stimulates the PITX2 P1 promoter transcription activation. Associates with chromatin to the PITX2 P1 promoter region. Binds DNA as a dimer. Can form homodimers or heterodimers with other AP-2 family members. Interacts with WWOX. Interacts with CITED4. Interacts with UBE2I. Interacts with RALBP1 in a complex also containing EPN1 and NUMB during interphase and mitosis. Interacts with KCTD1; this interaction represses transcription activation. Interacts (via C-terminus) with CITED2 (via C-terminus); the interaction stimulates TFAP2A- transcriptional activation. Interacts (via N-terminus) with EP300 (via N-terminus); the interaction requires CITED2. Belongs to the AP-2 family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 6p24.3
Cellular Component: centrosome; cytoplasm; Golgi apparatus; intracellular membrane-bound organelle; nucleoplasm; nucleus
Molecular Function: chromatin binding; protein binding; protein dimerization activity; protein homodimerization activity; sequence-specific DNA binding; transcription coactivator activity
Biological Process: embryonic cranial skeleton morphogenesis; embryonic forelimb morphogenesis; inner ear morphogenesis; kidney development; negative regulation of apoptosis; negative regulation of cell proliferation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; oculomotor nerve formation; palate development; positive regulation of bone mineralization; positive regulation of neuron apoptosis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of cell differentiation; sensory perception of sound; trigeminal nerve development
Disease: Branchiooculofacial Syndrome
Research Articles on AP-2alpha
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Product Notes
The AP-2alpha tfap2a (Catalog #AAA476871) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-AP-2alpha Mouse mAb reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's AP-2alpha can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the AP-2alpha tfap2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AP-2alpha, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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