Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Rat A Disintegrin And Metalloprotease 5 (ADAM5) Polyclonal Antibody | anti-ADAM5 antibody
Polyclonal Antibody to A Disintegrin And Metalloprotease 5 (ADAM5)
Gene Names
Adam5; tMDCII
Reactivity
Rat
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
A Disintegrin And Metalloprotease 5 (ADAM5); Polyclonal Antibody; Polyclonal Antibody to A Disintegrin And Metalloprotease 5 (ADAM5); anti-ADAM5 antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against ADAM5. It has been selected for its ability to recognize ADAM5 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSGSEF-CDFEEYCN GNDAYCVPDT FARNGQYCDS GQAFCYSGLC MTSNNQCMNL LGKYVRGASF ACYEEFNSRN DRFGNCIRKF CSFENSLCGK LVCTWPFKRL LMKDNMSAAY GQIRDDLCIS LYKGGRPLKT TLSTYSDMSE RDETFVKDGT ICGPDMFCLE TQCKETRFLV DFQQCNTSRD CNDHGVCNNF NHCHCDKGYN PPYCESVKGQ FGSIDDGHKY YIDEGKSAKQ QNRGIHPKQ
MGHHHHHHSGSEF-CDFEEYCN GNDAYCVPDT FARNGQYCDS GQAFCYSGLC MTSNNQCMNL LGKYVRGASF ACYEEFNSRN DRFGNCIRKF CSFENSLCGK LVCTWPFKRL LMKDNMSAAY GQIRDDLCIS LYKGGRPLKT TLSTYSDMSE RDETFVKDGT ICGPDMFCLE TQCKETRFLV DFQQCNTSRD CNDHGVCNNF NHCHCDKGYN PPYCESVKGQ FGSIDDGHKY YIDEGKSAKQ QNRGIHPKQ
Sequence Length
930
Applicable Applications for anti-ADAM5 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant ADAM5 (Cys413~Gln649) expressed in E.coli.
Cross Reactivity
Rat
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2085509
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:RatTissue))
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:RatTissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
79,907 Da
NCBI Official Full Name
disintegrin and metalloproteinase domain-containing protein 5
NCBI Official Synonym Full Names
ADAM metallopeptidase domain 5
NCBI Official Symbol
Adam5
NCBI Official Synonym Symbols
tMDCII
NCBI Protein Information
disintegrin and metalloproteinase domain-containing protein 5
UniProt Protein Name
Disintegrin and metalloproteinase domain-containing protein 5
Protein Family
UniProt Gene Name
Adam5
UniProt Synonym Gene Names
Tmdc2; tMDC II
NCBI Description
germ-cell specific metalloprotease-disintegrin that may play a role in cell-cell and cell-matrix interactions during spermatogenesis [RGD, Feb 2006]
Uniprot Description
ADAM5P: This is a non catalytic metalloprotease-like protein. 7 isoforms of the human protein are produced by alternative splicing.
Protein type: Protease
Chromosomal Location of Human Ortholog: 16q12.4
Cellular Component: integral component of membrane
Molecular Function: metalloendopeptidase activity
Biological Process: male gonad development; proteolysis
Similar Products
Product Notes
The ADAM5 adam5 (Catalog #AAA2005629) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to A Disintegrin And Metalloprotease 5 (ADAM5) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's A Disintegrin And Metalloprotease 5 (ADAM5) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the ADAM5 adam5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF-CDFEEY CN GNDAYCVPDT FARNGQYCDS GQAFCYSGLC MTSNNQCMNL LGKYVRGASF ACYEEFNSRN DRFGNCIRKF CSFENSLCGK LVCTWPFKRL LMKDNMSAAY GQIRDDLCIS LYKGGRPLKT TLSTYSDMSE RDETFVKDGT ICGPDMFCLE TQCKETRFLV DFQQCNTSRD CNDHGVCNNF NHCHCDKGYN PPYCESVKGQ FGSIDDGHKY YIDEGKSAKQ QNRGIHPKQ. It is sometimes possible for the material contained within the vial of "A Disintegrin And Metalloprotease 5 (ADAM5), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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