Rabbit anti-Rat A Disintegrin And Metalloprotease 10 (ADAM10) Polyclonal Antibody | anti-ADAM10 antibody

Polyclonal Antibody to A Disintegrin And Metalloprotease 10 (ADAM10)

Cat. Num. AAA2004491

• Gene Names: Adam10; MADM
• Reactivity: Rat
• Applications: Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
• Purity: Affinity Chromatography
• Synonyms: A Disintegrin And Metalloprotease 10 (ADAM10); Polyclonal Antibody; Polyclonal Antibody to A Disintegrin And Metalloprotease 10 (ADAM10); anti-ADAM10 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against ADAM10. It has been selected for its ability to recognize ADAM10 in immunohistochemical staining andwestern blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
• Concentration: 500ug/mL (varies by lot)
• Sequence: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.; MGHHHHHHSGSEF-TSG DKLNNNKFSL CSIRNISQVL EKKRNNCFVE SGQPICGNGM VEQGEECDCG YSDQCKDECC FDANQPEGKK CKLKPGKQCS PSQGPCCTAQ CAFKSKSEKC RDDSDCAKEG ICNGFTALCP ASDPKPNFTD CNRHTQVCIN GQCAGSICEK YDLEECTCAS SDGKDDKELC HVCCMKKMAP STCASTGSLQ WNKQFTGRTI TLQPGSPCND FRGYCDVFMR CRLVD
• Sequence Length: 1103

• Applicable Applications for anti-ADAM10 antibody: Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Western blotting: 1:100-400; Immunocytochemistry in formalin fixed cells: 1:100-500; Immunohistochemistry in formalin fixed frozen section: 1:100-500; Immunohistochemistry in paraffin section: 1:50-200; Enzyme-linked Immunosorbent Assay: 1:100-200
• Immunogen: Recombinant ADAM10 (Thr218~Asp445) expressed in E.coli.
• Cross Reactivity: Rat
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2047079
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin- embedded kidney tissue))

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Rat Spinal cord Tissue))

NCBI and Uniprot Product Information

• NCBI GI #: 37096570
• NCBI GeneID: 29650
• UniProt Accession #: Q10743
• Molecular Weight: 60,445 Da
• NCBI Official Full Name: Disintegrin and metalloproteinase domain-containing protein 10
• NCBI Official Synonym Full Names: ADAM metallopeptidase domain 10
• NCBI Official Symbol: Adam10
• NCBI Official Synonym Symbols: MADM
• NCBI Protein Information: disintegrin and metalloproteinase domain-containing protein 10
• UniProt Protein Name: Disintegrin and metalloproteinase domain-containing protein 10
• Protein Family: Disintegrin and metalloproteinase domain-containing protein
• UniProt Gene Name: Adam10
• UniProt Synonym Gene Names: Madm; ADAM 10

NCBI Description

may act as a disintegrin-metalloprotease related to the reprolysin family [RGD, Feb 2006]

Uniprot Description

ADAM10: Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch. Responsible for the FasL ectodomain shedding and for the generation of the remnant ADAM10-processed FasL (FasL APL) transmembrane form. Also cleaves the ectodomain of the integral membrane proteins CORIN and ITM2B. May regulate the EFNA5-EPHA3 signaling (By similarity)

Protein type: Cell adhesion; Cell surface; EC 3.4.24.81; Membrane protein, integral; Motility/polarity/chemotaxis; Protease; Vesicle

Chromosomal Location of Human Ortholog: 8q24

Cellular Component: cell surface; cytoplasm; dendrite; extracellular space; focal adhesion; Golgi apparatus; Golgi membrane; Golgi-associated vesicle; integral component of membrane; membrane; nucleus; plasma membrane; postsynaptic density; trans-Golgi network

Molecular Function: endopeptidase activity; metal ion binding; metalloendopeptidase activity; metallopeptidase activity; protein homodimerization activity; protein kinase binding; SH3 domain binding

Biological Process: amyloid-beta formation; constitutive protein ectodomain proteolysis; in utero embryonic development; membrane protein ectodomain proteolysis; monocyte activation; negative regulation of cell adhesion; Notch signaling pathway; PMA-inducible membrane protein ectodomain proteolysis; positive regulation of cell growth; positive regulation of cell migration; positive regulation of cell proliferation; protein amino acid phosphorylation; protein processing; regulation of osteoclast differentiation