A Disintegrin And Metalloprotease 5 (ADAM5) Recombinant Protein | ADAM5 recombinant protein

Recombinant A Disintegrin And Metalloprotease 5 (ADAM5)

Cat. Num. AAA2010896

• Gene Names: Adam5; tMDCII
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: A Disintegrin And Metalloprotease 5 (ADAM5); Recombinant A Disintegrin And Metalloprotease 5 (ADAM5); ADAM5 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-CDFEEYCN GNDAYCVPDT FARNGQYCDS GQAFCYSGLC MTSNNQCMNL LGKYVRGASF ACYEEFNSRN DRFGNCIRKF CSFENSLCGK LVCTWPFKRL LMKDNMSAAY GQIRDDLCIS LYKGGRPLKT TLSTYSDMSE RDETFVKDGT ICGPDMFCLE TQCKETRFLV DFQQCNTSRD CNDHGVCNNF NHCHCDKGYN PPYCESVKGQ FGSIDDGHKY YIDEGKSAKQ QNRGIHPKQ
• Sequence Length: 709

• Applicable Applications for ADAM5 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Cys413~Gln649 (Accession # Q5BK84) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 61657897
• NCBI GeneID: 498654
• NCBI Accession #: NP_064699.1
• NCBI GenBank Nucleotide #: NM_020303.1
• UniProt Accession #: Q5BK84
• Molecular Weight: 28.4kDa
• NCBI Official Full Name: disintegrin and metalloproteinase domain-containing protein 5
• NCBI Official Synonym Full Names: a disintegrin and metallopeptidase domain 5
• NCBI Official Symbol: Adam5
• NCBI Official Synonym Symbols: tMDCII
• NCBI Protein Information: disintegrin and metalloproteinase domain-containing protein 5; tMDC II; ADAM metallopeptidase domain 5; a disintegrin and metalloprotease domain 5; a disintegrin and metalloprotease domain (ADAM) 5; transmembrane metalloproteinase-like, disintegrin-like, and cysteine-rich protein II
• UniProt Protein Name: Disintegrin and metalloproteinase domain-containing protein 5
• Protein Family: Disintegrin and metalloproteinase domain-containing protein
• UniProt Gene Name: Adam5
• UniProt Synonym Gene Names: Tmdc2; tMDC II
• UniProt Entry Name: ADAM5_RAT

NCBI Description

germ-cell specific metalloprotease-disintegrin that may play a role in cell-cell and cell-matrix interactions during spermatogenesis [RGD, Feb 2006]

Uniprot Description

ADAM5P: This is a non catalytic metalloprotease-like protein. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease

Cellular Component: integral to membrane

Molecular Function: zinc ion binding; metalloendopeptidase activity

Biological Process: proteolysis