Western Blot (WB)
(Western Blot (WB) analysis of IgG4 Mouse mAb at 1:2000 dilutionLane1:The plasma lysate of Human)
Rabbit anti-Human IgG4 Monoclonal Antibody | anti-IgG4 antibody
IgG4 Rabbit Monoclonal Antibody
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Protein A affinity purified
Synonyms
IgG4; Monoclonal Antibody; IgG4 Rabbit Monoclonal Antibody; Ig gamma-4 chain C region; IGHG4; anti-IgG4 antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG
Specificity
This antibody detects endogenous levels of IgG4 and does not cross-react with related proteins.
Purity/Purification
Protein A affinity purified
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
327
Applicable Applications for anti-IgG4 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:1000-1:5000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
Recombinant antibody.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of IgG4 Mouse mAb at 1:2000 dilutionLane1:The plasma lysate of Human)
Western Blot (WB)
(Western Blot (WB) analysis of IgG4 Mouse mAb at 1:2000 dilutionLane1:The plasma lysate of Human)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IgG4 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IgG4 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IgG4 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IgG4 antibody. Counter stained with hematoxylin.)
Related Product Information for anti-IgG4 antibody
The regions of relatively constant sequence beyond the variable regions of immunoglobulins are termed constant regions (C regions) and are present in both the heavy and light chains. With very few exceptions, the sites of attachment for carbohydrates on immunoglobulins are located in these C regions. These regions also function to hold the variable regions together by using the disulfide bond between them. The C regions facilitate interaction with the antigen by increasing the maximum rotation of the immunoglobulin arms. Several studies have shown that a large population of patients with recurrent respiratory tract infection have low IgG4 concentrations.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
~ 36kDa
NCBI Official Full Name
Immunoglobulin heavy constant gamma 4
NCBI Official Synonym Full Names
immunoglobulin heavy constant gamma 4 (G4m marker)
NCBI Official Symbol
IGHG4
UniProt Protein Name
Ig gamma-4 chain C region
UniProt Gene Name
IGHG4
UniProt Entry Name
IGHG4_HUMAN
Uniprot Description
IGHG4:
Chromosomal Location of Human Ortholog: 14q32.33
Cellular Component: extracellular space; extracellular region
Molecular Function: antigen binding
Biological Process: innate immune response; complement activation, classical pathway; complement activation
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Product Notes
The IgG4 ighg4 (Catalog #AAA3014165) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IgG4 Rabbit Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IgG4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:1000-1:5000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the IgG4 ighg4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgG4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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