Human Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) ELISA Kit | CCL3L1 elisa kit

Human Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) ELISA Kit

Cat. Num. AAA452074

• Gene Names: CCL3L3; LD78; 464.2; SCYA3L; G0S19-2; SCYA3L1; D17S1718; LD78BETA
• Reactivity: Human
• Synonyms: Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1); Human Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) ELISA Kit; CCL3-L1; LD78; SCYA3L; SCYA3L1; MIP1AP; MIP1-Alpha-P; Small Inducible Cytokine A3-Like 1; Macrophage Inflammatory Protein 1-Alpha-P; Tonsillar Lymphocyte LD78 Beta; CCL3L1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CCL3L1. No significant cross-reactivity or interference between CCL3L1 and analogues was observed.
• Sequence Length: 93

• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 78.125-5000pg/mL
• Sensitivity: 32pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CCL3L1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CCL3L1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CCL3L1 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CCL3L1 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CCL3L1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CCL3L1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CCL3L1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CCL3L1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 93589094
• NCBI GeneID: 414062
• NCBI Accession #: NP_001001437.2
• NCBI GenBank Nucleotide #: NM_001001437.3
• UniProt Accession #: P16619; Q3B7A8; Q53YA5; Q96I68; A6NGK6; B2RUX4
• Molecular Weight: 10,161 Da
• NCBI Official Full Name: C-C motif chemokine 3-like 1
• NCBI Official Synonym Full Names: C-C motif chemokine ligand 3 like 3
• NCBI Official Symbol: CCL3L3
• NCBI Official Synonym Symbols: LD78; 464.2; SCYA3L; G0S19-2; SCYA3L1; D17S1718; LD78BETA
• NCBI Protein Information: C-C motif chemokine 3-like 1
• UniProt Protein Name: C-C motif chemokine 3-like 1
• Protein Family: C-C motif chemokine
• UniProt Gene Name: CCL3L1
• UniProt Synonym Gene Names: D17S1718; G0S19-2; SCYA3L1

NCBI Description

This gene is one of several cytokine genes that are clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins that function in inflammatory and immunoregulatory processes. The protein encoded by this gene binds to several chemokine receptors, including chemokine binding protein 2 and chemokine (C-C motif) receptor 5 (CCR5). CCR5 is a co-receptor for HIV, and binding of this protein to CCR5 inhibits HIV entry. The copy number of this gene varies among individuals, where most individuals have one to six copies, and a minority of individuals have zero or more than six copies. There are conflicting reports about copy number variation of this gene and its correlation to disease susceptibility.[provided by RefSeq, Apr 2014]

Uniprot Description

Chemotactic for lymphocytes and monocytes. Is a ligand for CCR1, CCR3 and CCR5. Is an inhibitor of HIV-1-infection. The processed form LD78-beta(3-70) shows a 20-fold to 30-fold higher chemotactic activity and is a very potent inhibitor of HIV-1-infection. LD78-beta(3-70) is also a ligand for CCR1, CCR3 and CCR5.