Western Blot (WB)
(Western blot analysis of extracts of HeLa cells, using ADAM5 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
Rabbit anti-Human ADAM5 Polyclonal Antibody | anti-ADAM5 antibody
ADAM5 Polyclonal Antibody
Gene Names
ADAM5; ADAM5P; TMDCII
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purification
Synonyms
ADAM5; Polyclonal Antibody; ADAM5 Polyclonal Antibody; ADAM5P; TMDCII; anti-ADAM5 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Concentration
1mg/ml (varies by lot)
Sequence Length
412
Applicable Applications for anti-ADAM5 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IHC: 1:50 - 1:200
Species
Human
Immunogen
Recombinant Protein
Immunogen
Recombinant protein of human ADAM5
Calculated Molecular Weight
47kDa
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Western Blot (WB)
(Western blot analysis of extracts of HeLa cells, using ADAM5 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
Western Blot (WB)
(Western blot analysis of extracts of HeLa cells, using ADAM5 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
Related Product Information for anti-ADAM5 antibody
ADAM5(putative disintegrin and metalloproteinase domain-containing protein 5) is also named as ADAM5P, TMDC2(tMDCII). The tMDC II, abundantly expressed, sperm surface MDC proteins identified in rodent and macaque systems, is also nonfunctional in the human. It has 7 isoforms produced by alternative splicing. It is expressed in testis highly by northern blot. This antibody is specific to ADAM5.
Product Categories/Family for anti-ADAM5 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
412
NCBI Official Full Name
Putative disintegrin and metalloproteinase domain-containing protein 5
NCBI Official Synonym Full Names
ADAM metallopeptidase domain 5 (pseudogene)
NCBI Official Symbol
ADAM5
NCBI Official Synonym Symbols
ADAM5P; TMDCII
UniProt Protein Name
Putative disintegrin and metalloproteinase domain-containing protein 5
Protein Family
UniProt Gene Name
ADAM5
UniProt Synonym Gene Names
ADAM5P; TMDC2; tMDC II
UniProt Entry Name
ADAM5_HUMAN
Uniprot Description
ADAM5P: This is a non catalytic metalloprotease-like protein. 7 isoforms of the human protein are produced by alternative splicing.
Protein type: Protease
Chromosomal Location of Human Ortholog: 8p11.22
Molecular Function: zinc ion binding; metalloendopeptidase activity
Biological Process: proteolysis
Similar Products
Product Notes
The ADAM5 adam5 (Catalog #AAA9126626) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ADAM5 Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ADAM5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500 - 1:2000 IHC: 1:50 - 1:200. Researchers should empirically determine the suitability of the ADAM5 adam5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ADAM5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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