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Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Rabbit anti-Human A Disintegrin And Metalloprotease 15 (ADAM15) Polyclonal Antibody | anti-ADAM15 antibody

Polyclonal Antibody to A Disintegrin And Metalloprotease 15 (ADAM15)

Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
A Disintegrin And Metalloprotease 15 (ADAM15); Polyclonal Antibody; Polyclonal Antibody to A Disintegrin And Metalloprotease 15 (ADAM15); anti-ADAM15 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against ADAM15. It has been selected for its ability to recognize ADAM15 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSGSEF- SLGDGEPCAG GQAVCMHGRC ASYAQQCQSL WGPGAQPAAP LCLQTANTRG NAFGSCGRNP SGSYVSCTPR DAICGQLQCQ TGRTQPLLGS IRDLLWETID VNGTELNCSW VHLDLGSDVA QPLLTLPGTA CGPGLVCIDH RCQRVDLLGA QECRSKCHGH GVCDSNRHCY CEEGWAPPDC TTQLKATSSL TTGLLLSLLV LLVLVMLGAS YWYRARLHQR LCQLKGPTCQ YRAAQSGPSE R
Sequence Length
950
Applicable Applications for anti-ADAM15 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant ADAM15 (Ser211~Arg451) expressed in E.coli.
Cross Reactivity
Human
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2081758
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DABstainingonIHC-P.Samples:HumanTissue))

Immunohistochemistry (IHC) (DABstainingonIHC-P.Samples:HumanTissue))

NCBI and Uniprot Product Information

NCBI Description

This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. ADAMTS family members share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The protein encoded by this gene has a high sequence similarity to the proteins encoded by ADAMTS1 and ADAMTS8. [provided by RefSeq, Jul 2008]

Uniprot Description

ADAMTS15:

Protein type: Secreted, signal peptide; Protease; Motility/polarity/chemotaxis; EC 3.4.24.-; Secreted; Extracellular matrix

Chromosomal Location of Human Ortholog: 11q25

Cellular Component: proteinaceous extracellular matrix

Molecular Function: zinc ion binding; metalloendopeptidase activity

Biological Process: proteolysis

Research Articles on ADAM15

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Product Notes

The ADAM15 (Catalog #AAA2006125) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to A Disintegrin And Metalloprotease 15 (ADAM15) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's A Disintegrin And Metalloprotease 15 (ADAM15) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the ADAM15 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF- SLGDGEPCAG GQAVCMHGRC ASYAQQCQSL WGPGAQPAAP LCLQTANTRG NAFGSCGRNP SGSYVSCTPR DAICGQLQCQ TGRTQPLLGS IRDLLWETID VNGTELNCSW VHLDLGSDVA QPLLTLPGTA CGPGLVCIDH RCQRVDLLGA QECRSKCHGH GVCDSNRHCY CEEGWAPPDC TTQLKATSSL TTGLLLSLLV LLVLVMLGAS YWYRARLHQR LCQLKGPTCQ YRAAQSGPSE R. It is sometimes possible for the material contained within the vial of "A Disintegrin And Metalloprotease 15 (ADAM15), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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