Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Purity
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), ELISA (EIA)
Purity
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Purity
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Purity
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Purity
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Purity
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Immunohistochemistry (IHC) (MBS9600651 at 1/100 staining Rat colorectal tissue by IHC-P. Thesample was formaldehyde fixed and a heat mediated antigenretrieval step in citrate buffer was performed. The sample wasthen blocked and incubated with the primary antibody at 4°Covernight. An HRP conjugated anti-Rabbit antibody was usedas the secondary antibody.)
Western Blot (WB) (Western blot analysis of extracts from K562 cells, usingPhospho-Beclin-1 (Ser15) Antibody. Lane1 was treated withphospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)
Western Blot (WB) (Western blot analysis of extracts from UV treated RAW264.7 cells, using Phospho-STING (Ser366) Antibody. The lane on the left was treated with blocking peptide.)
Immunohistochemistry (IHC) (MBS9610778 at 1/100 staining human lung cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
Immunofluorescence (IF) (MBS9610778 staining HepG2 cells(4h of LPS treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9610778 1:200) and mouse anti-beta tubulin Ab(MBS9610328 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat antimouse IgG(H+L) Ab(Green) were used as the secondary)
Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded human brain, using Bax (Phospho-Ser184) Antibody. The picture on the right is blocked with the phospho peptide.)
Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Akt (Phospho-Ser473) Antibody. The picture on the right is blocked with the phospho peptide.)
Western Blot (WB) (Western blot analysis of lysates from HeLa cells treated with heat shock, using Akt (Phospho-Ser473) Antibody. The lane on the left is blocked with the phospho peptide.)
Western Blot (WB) (Western blot analysis of extracts from HepG2 using TIE2 (Phospho-Tyr992) Antibody.The lane on the right is treated with the antigen-specific peptide.)
Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using CREB (Phospho-Ser133) Antibody. The picture on the right is blocked with the phospho peptide.)
Western Blot (WB) (Western blot analysis of lysates from Jurkat and 293 cells treated with UV, using CREB (Phospho-Ser133) Antibody. The lane on the right is blocked with the phospho peptide.)
Western Blot (WB) (Western blot analysis of extracts from HT29 cells, untreated or treated with UV using Ephrin-B2(Phospho-Tyr316) Antibody.)
Western Blot (WB) (Western blot analysis of GSK3 beta (pS9) expression in MCF7 (A), NIH3T3 (B), PC12 (C) whole cell lysates.)
Immunohistochemistry (IHC) (Immunohistochemical analysis of GSK3 beta (pS9) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Western Blot (WB) (Western blot analysis of Phospho-eIF2 alpha (Ser51) expression in various lysates)
Immunohistochemistry (IHC) (MBS9600859 at 1/100 staining human placenta tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
)
Immunohistochemistry (IHC) (MBS9600859 at 1/100 staining human appendiceal tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
)
Immunohistochemistry (IHC) (MBS9600859 at 1/100 staining human lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
)
Immunohistochemistry (IHC) (MBS9600859 at 1/100 staining mouse gastric tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
)
Immunohistochemistry (IHC) (MBS9600859 at 1/100 staining mouse kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
)
Western Blot (WB) (MCF-7 and MDA-MB-231 cells were treated with the indicated concentrations of BA for 24h, and the protein levels of ER stress-associated signals were stimulated by BA in a dose-dependent manner, including GRP78, p-PERK/PERK, p-eIF2alpha/eIF2alpha, CHOP, and caspase-12.)
Immunohistochemistry (IHC) (MBS9600859 at 1/100 staining mouse lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
)
Immunohistochemistry (IHC) (MBS9600859 at 1/100 staining rat uterine tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
)
Immunohistochemistry (IHC) (MBS9600859 at 1/100 staining rat kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
)
Immunohistochemistry (IHC) (MBS9600859 at 1/100 staining rat ovarian tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
)
Immunofluorescence (IF) (MBS9600859 staining K562 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Western Blot (WB) (Western blot analysis of eIF2 alpha phosphorylation expression in IFN-alpha treated K562 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB) (Western blot analysis of p21 (pT145) expression in HT1080 EGF-treated (A), HeLa EGF-treated (B) whole cell lysates.)
Immunohistochemistry (IHC) (Immunohistochemical analysis of p21 (pT145) staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunofluorescence (IF) (Immunofluorescent analysis of p21 (pT145) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Western Blot (WB) (Western blot analysis of ATF4 (pS245) expression in MCF7 (A), mouse heart (B), rat heart (C) whole cell lysates.)
Immunohistochemistry (IHC) (Immunohistochemical analysis of ATF4 (pS245) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC) (At 1/100 staining mouse heart tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
Immunohistochemmistry (IHC) (At 1/100 staining rat heart tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
Immunohistochemistry (IHC) (At 1/100 staining human lung cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
Western Blot (WB) (Western blot analysis of Phospho-Casein Kinase II (Thr360) in lysates of HeLa, using Phospho-Casein Kinase II (Thr360) Antibody.)
Immunofluorescence (IF) (MBS9610696 staining HepG2 cells(4h of LPS treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9610696 1:200) and mouse anti-beta tubulin Ab(MBS9610328 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L)Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).)
Western Blot (WB) (Western blot analysis of extracts from Jurkat cells untreated or treated with H2O2 using eIF2a(Phospho-Ser51) Antibody.)
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eIF2alpha (Phospho-Ser51) Antibody (left) or the same antibody preincubated with blocking peptide #51279 (right).)
Immunofluorescence (IF) (Immunofluorescence staining of methanol-fixed Hela cells using eIF2a(Phospho-Ser51) Antibody.)
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using eIF2alpha (Phospho-Ser51) Antibody.)
Immunohistochemistry (IHC) (MBS9601077 at 1/50 staining human lung cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC) (MBS9601077 at 1/100 staining human prostate cancer tissues sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hou)
Immunohistochemistry (IHC) (MBS9601077 at 1/50 staining human lung cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB) (Western blot analysis of mTOR phosphorylation expression in EGF treated HeLa whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
Immunohistochemistry (IHC) (Phospho-mTOR (Ser2448) Antibody for IHC in human prostate tissue)
Western Blot (WB) (Western blot analysis of mTOR phosphorylation expression in mouse liver tissue lysates, The lane on the right is treated with the antigen-specific peptide.)
Western Blot (WB) (Western blot analysis of extracts from 293 cells untreated or treated with PMA using Shc1(Phospho-Tyr427) Antibody.)
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Shc1(Phospho-Tyr427) Antibody (left) or the same antibody preincubated with blocking peptide(right).)
Immunofluorescence (IF) (Immunofluorescence staining of methanol-fixed Hela cells using Shc1(Phospho-Tyr427) Antibody.)
Immunohistochemistry (IHC) (MBS9600900 at 1/100 staining human testis by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
Immunofluorescence (IF) (MBS9600900 staining Hela cells (4h of LPS treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9600900: 1:200) and mouse anti-beta tubulin Ab(MBS9610328 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary Ab. The nuclear counter stain is DAPI(blue).)
Western Blot (WB) (Western blot analysis of extracts of various tissue sample, using Phospho-GSK3 beta (Ser9) Antibody.)
Western Blot (WB) (Western blot analysis of extracts of various tissue sample, using Phospho-GSK3 beta (Ser9) Antibody.)
Immunofluorescence (IF) (MBS9600628 staining lovo cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37 degree C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37 degree C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)
Immunohistochemistry (IHC) (MBS9600628 at 1/100 staining mouse gastric tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC) (MBS9600628 at 1/100 staining mouse kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC) (MBS9600628 at 1/100 staining mouse brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC) (MBS9600628 at 1/100 staining human pancreatic tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC) (MBS9600628 at 1/100 staining human gastric tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC) (MBS9600628 at 1/100 staining human glioma tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC) (MBS9600628 at 1/100 staining rat uterine tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC) (MBS9600628 at 1/100 staining rat gastric tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC) (MBS9600628 at 1/100 staining rat ovarian tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB) (Western blot analysis of extracts of various tissue sample, using Phospho-GSK3 beta (Ser9) Antibody.)
Immunofluorescence (IF) (MBS9600628 staining HuvEc by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Western Blot (WB) (Western blot analysis of extracts from A431 cells,untreated or treated with EGF,100ng/ml,using EGFR Antibody (pY1016)(upper) or Beta-actin (lower).)
Dot Blot (DB) (Dot blot analysis of anti-EGFR B1 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)
Western Blot (WB) (Western blot analysis of lysates from 293 cells treated with EGF 200ng/ml 30', using FAK (Phospho-Tyr397) Antibody. The lane on the right is blocked with the phospho peptide.)
Western Blot (WB) (Western blot analysis of extracts from HepG2 cells using FGFR1/2 (Phospho-Tyr463/466) Antibody.The lane on the right is treated with the antigen-specific peptide.)
Immunofluorescence (IF) (MBS9601204 staining HuvEc by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Western Blot (WB) (Western blot analysis of IL-10R alpha phosphorylation expression in HuvEc whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB) (Western blot analysis of Phospho-IL-10R alpha (Tyr496) Antibody expression in HuvEc cells lysates.The lane on the right is treated with the antigen-specific peptide.)
Western Blot (WB) (Western blot analysis of extracts from HepG2 cells untreated or treated with EGF using AKT1/AKT2/AKT3(phospho-Tyr315/316/312) Antibody.)
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AKT1/AKT2/AKT3(Phospho-Tyr315/316/312) Antibody (left) or the same antibody preincubated with blocking peptide(right).)
Immunofluorescence (IF) (Immunofluorescence staining of methanol-fixed Hela cells using AKT1/AKT2/AKT3(phospho-Tyr315/316/312) Antibody.)
Western Blot (WB) (Western blot analysis of extracts from 3T3 cells, treated with EGF or calf intestinal phosphatase (CIP), using AKT1/AKT2/AKT3 (phospho-Tyr315/316/312) Antibody.)
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using SHP-1(Phospho-Tyr536) Antibody (left) or the same antibody preincubated with blocking peptide(right).)
Immunofluorescence (IF) (Immunofluorescence staining of methanol-fixed Hela cells using SHP-1(Phospho-Tyr536) Antibody.)
Western Blot (WB) (Western blot analysis of extracts of HepG2 cells, using Phospho-PDGFRA-Y754 antibody at 1:1000 dilution. HepG2 cells were treated by EGF (100ng/ml) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)
Immunofluorescence (IF) (Immunofluorescence analysis of COS7 cells, using CaMK2 (Phospho-Thr286) Antibody. The picture on the right is blocked with the phospho peptide.)
Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded human brain, using CaMK2 (Phospho-Thr286) Antibody. The picture on the right is blocked with the phospho peptide.)
Western Blot (WB) (Western blot analysis of lysates from K562 cells, using CaMK2 (Phospho-Thr286) Antibody. The lane on the right is blocked with the phospho peptide.)
Immunofluorescence (IF) (MBS9600779 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Western Blot (WB) (Western blot analysis of Chk1 phosphorylation expression in MCF7 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
Immunohistochemistry (IHC) (MBS9600779 at 1/100 staining mouse spleen tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC) (MBS9600779 at 1/100 staining mouse brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC) (MBS9600779 at 1/100 staining human vascular cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC) (MBS9600779 at 1/100 staining human lymphoid tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC) (MBS9600779 at 1/100 staining human appendiceal tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC) (MBS9600779 at 1/100 staining rat kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC) (MBS9600779 at 1/100 staining rat spleen tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC) (MBS9600779 at 1/100 staining rat liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB) (Western blot analysis of extracts from 293 cells untreated or treated with forskolin using PAK1(Phospho-Thr212) Antibody.)
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PAK1(Phospho-Thr212) Antibody (left) or the same antibody preincubated with blocking peptide(right).)
Immunofluorescence (IF) (Immunofluorescence staining of methanol-fixed Hela cells using PAK1(Phospho-Thr212) Antibody.)
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eNOS(Phospho-Ser1177) Antibody (left) or the same antibody preincubated with blocking peptide(right).)
Immunofluorescence (IF) (Immunofluorescence staining of methanol-fixed Hela cells using eNOS(Phospho-Ser1177) Antibody.)
Western blot (Western blot analysis of extracts from 3T3 cells treated with EGF, HWEC cells treated with VEGF and JK cells using eNOS (Phospho-Ser1177) Antibody #MBS9414700.)
Western Blot (WB) (Western blot analysis of extracts from 293 cells untreated or treated with serum using FKHR(Phospho-Ser256) Antibody.)
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using FKHR (Phospho-Ser256) Antibody (left) or the same antibody preincubated with blocking peptide #51115 (right).)
Immunofluorescence (IF) (Immunofluorescence staining of methanol-fixed MCF7 cells using FKHR(Phospho-Ser256) Antibody.)
Immunofluorescence (IF) (Immunofluorescence staining of methanol-fixed MEF cells untreated or treated with LY2904 using FKHR (Phospho-Ser256) Antibody.)
Western Blot (Western blot analysis of extracts from MCF-7 cells, usingPhospho-MFF (Ser172/146) Antibody. Lane1 was treated withphospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)
Immunohistochemistry (IHC) (MBS9600693 at 1/100 staining Human esophageal cancer by IHC-P.The sample was formaldehyde fixed and a heat mediatedantigen retrieval step in citrate buffer was performed. Thesample was then blocked and incubated with the primaryantibody at 4°C overnight. An HRP conjugated anti-Rabbitantibody was used as the secondary antibody.)
Western Blot (WB) (Western blot analysis of lysates from A431 cell line,untreated or treated with EGF,using phospho-ErBB2(Y877) Antibody (upper) or Beta-actin (lower).)
Dot Blot (DB) (Dot blot analysis of Phospho-ERBB2-Y877 Antibody Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)
Western Blot (WB) (Western blot analysis of extracts from JurKat cells using HCK (Phospho-Tyr522) Antibody.The lane on the right is treated with the antigen-specific peptide.)
Western Blot (WB) (Western blot analysis of BMX (pY566) expression in HeLa (A) whole cell lysates.)
Immunohistochemistry (IHC) (Immunohistochemical analysis of BMX (pY566) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunofluorescence (IF) (Immunofluorescent analysis of BMX (pY566) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Western Blot (WB) (Western blot analysis of SGK1 (pS422) expression in A549 Insulin-treated (A), K562 Insulin-treated (B) whole cell lysates.)
Immunohistochemistry (IHC) (Immunohistochemical analysis of SGK1 (pS422) staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunofluorescence (IF) (Immunofluorescent analysis of SGK1 (pS422) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Western Blot (WB) (Western blot analysis of extracts from MCF cells untreated or treated with EGF using p70 S6 Kinase(Phospho-Ser424) Antibody.)
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p70 S6 Kinase(Phospho-Ser424) Antibody (left) or the same antibody preincubated with blocking peptide(right).)
Immunofluorescence (IF) (Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic, centrosomal and nuclear staining using p70 S6 Kinase(Phospho-Ser424) Antibody.)
Immunofluorescence (IF) (MBS9601009 staining Hela cells(4h of LPS treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab (MBS9601009 1:200) and mouse anti-beta tubulin Ab(MBS9610328 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
Western Blot (WB) (Western blot analysis of IL-8R beta/CDw128 beta phosphorylation expression in PMA treated NIH-3T3 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
Immunofluorescence (IF) (MBS9601009 staining COS7 cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37 degree C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37 degree C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)
Immunohistochemistry (IHC) (MBS9601009 at 1/100 staining Mouse intestine tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunofluorescence (IF) (MBS9601009 staining NIH-3T3 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Western Blot (WB) (Western blot analysis of Phospho-IL-8R beta/CDw128 beta (Ser347) expression in Mouse kidney lysate)
Western Blot (WB) (Western blot analysis of extracts from 293 cells, using Phospho-GJA1-S368 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human breast carcinoma using Phospho-GJA1-S368 antibody.)
Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded human brain, using DDR1 (Phospho-Tyr513) Antibody. The picture on the right is blocked with the phospho peptide.)
Western Blot (WB) (Western blot analysis of lysates from HepG2 cells treated with Na3VO4 0.3mM 40', using DDR1 (Phospho-Tyr513) Antibody. The lane on the right is blocked with the phospho peptide.)
Western Blot (WB) (Western blot analysis of extracts from HepG2 cells using Phospho-STAT4-Y693 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human breast carcinoma using Phospho-STAT4-Y693 antibody.)
Western Blot (WB) (Western blot analysis of extracts of HeLa cells, using Phospho-RELA-S311 antibody at 1:2000 dilution. HeLa cells were treated by IFN-α (100ng/ml) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Enhanced Kit.Exposure time: 90s.)
Western Blot (WB) (Western blot analysis of PAK4 (pS474) expression in HEK293T EGF-treated (A), Raw264.7 EGF-treated (B), PC12 EGF-treated (C) whole cell lysates.)
Immunofluorescence (IF) (Immunofluorescent analysis of PAK4 (pS474) staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Western Blot (WB) (Western blot analysis of extracts from 3T3 cells treated with EGF using MER/SKY (Phospho-Tyr749/681) Antibody.The lane on the right is treated with the antigen-specific peptide.)
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human brain tissue using MER/SKY (Phospho-Tyr749/681) antibody (left)or the same antibody preincubated with blocking peptide (right).)
Western Blot (WB) (Western blot analysis of extracts of 293 cells, using Phospho-MAP2K1-T286 antibody at 1:1000 dilution. 293 cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)
Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded human brain, using SLP-76 (Phospho-Tyr128) Antibody. The picture on the right is blocked with the phospho peptide.)
Western Blot (WB) (Western blot analysis of lysates from K562 cells treated with Na3VO4 0.3nM 40', using SLP-76 (Phospho-Tyr128) Antibody. The lane on the right is blocked with the phospho peptide.)
Immunohistochemistry (IHC) (MBS9600841 at 1/100 staining mouse gastric tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
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Immunohistochemistry (IHC) (MBS9600841 at 1/100 staining mouse brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
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Immunohistochemistry (IHC) (MBS9600841 at 1/100 staining mouse testicular tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
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Immunohistochemistry (IHC) (MBS9600841 at 1/100 staining human skin tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
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Immunohistochemistry (IHC) (MBS9600841 at 1/100 staining human brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
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Immunohistochemistry (IHC) (MBS9600841 at 1/100 staining human TB tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
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Immunofluorescence (IF) (MBS9600841 staining MDA-MB-231 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunohistochemistry (IHC) (MBS9600841 at 1/100 staining rat kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
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Immunohistochemistry (IHC) (MBS9600841 at 1/100 staining rat ovarian tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
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Immunohistochemistry (IHC) (MBS9600841 at 1/100 staining rat appendiceal tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
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Western Blot (WB) (Western blot analysis of HER2 phosphorylation expression in EGF treated SK-OV3 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB) (Western blot analysis of extracts of various tissue samples, using Phospho-HER2 (Tyr1248) Antibody.)
Immunohistochemistry (IHC) (At 1/100 staining mouse liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
Immunohistochemmistry (IHC) (At 1/100 staining rat kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
Immunohistochemistry (IHC) (At 1/100 staining human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
Western Blot (WB) (Western blot analysis of Phospho-MYOD (Thr115) in lysates of Jurkat Ca+ 40nM 30', using Phospho-MYOD (Thr115) Antibody.)
Immunofluorescence (IF) (MBS9610595 staining H2O2 treated HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9610595) and mouse anti-beta tubulin Ab( MBS9610328 ) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary Ab. The nuclear counter stain is DAPI(blue).)
Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using FANCA (Phospho-Ser1149) antibody. Western blot analysis of extracts from HELA cells treated with IGF (100ng/ml, 10mins) and HUVEC cells treated with EGF (200ng/ml, 30mins), using FANCA (Phospho-Ser1149) antibody.)
Immunofluorescence (IF) (P-peptide-+ Immunofluorescence analysis of HeLa cells, using FANCA (Phospho-Ser1149) antibody.)
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