Rabbit anti-Human Beclin1 (Ser234) Polyclonal Antibody | anti-BECN1 antibody

Anti-Phospho-Ser234 Beclin-1

Cat. Num. AAA502210

• Gene Names: BECN1; ATG6; VPS30; beclin1
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
• Synonyms: Beclin1 (Ser234); Polyclonal Antibody; Anti-Phospho-Ser234 Beclin-1; anti-BECN1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Specific for the ~60k beclin1 protein phosphorylated at Ser234. Immunolabeling of the beclin-1 band is decreased in the beclin-1 alanine substitution mutant. Additional bands may be observed in non-immunoprecipitated lysates.
• Purity/Purification: Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
• Form/Format: 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol.
• Sequence Length: 450

• Applicable Applications for anti-BECN1 antibody: Western Blot (WB)
• Application Notes: Quality Control: Western blots performed on each lot.; WB: 1:250
• Antigen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser234 of human beclin-1.
• Immunogen Information: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser234 conjugated to KLH
• Immunogen Species: Human
• Reactivity Assumed Based on 100% Sequence Homology: Bovine, canine, mouse, non-human primate, rat, sheep
• Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with recombinant HeLa and 293T cells. It is anticipated that the antibody will react with bovine, canine, mouse, non-human primate, rat, and sheep tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
• Biological Significance: Beclin-1 plays a key role in tumor suppression and autophagy. It has been suggested that autophagy proteins such as beclin-1 can inhibit tumorigenesis and that decreased expression of these proteins may contribute to the development of human malignancies (Liang et al., 1999). Beclin-1 has been shown to interact with 14-3-3 proteins through Akt-mediated phosphorylation at Ser234 (Wang et al., 2012).
• Preparation and Storage: Store at -20 degree C; stable for at least one year

Testing Data

Related Product Information for anti-BECN1 antibody

Affinity purified rabbit polyclonal antibody

References

• Liang, XH, Jackson S, Seaman M, Brown K, Kempkes B, Hibshoosh H, Levine B. (1999) Induction of autophagy and inhibition of tumorigenesis by beclin-1. Nature 402(6762):672-676.
• Richard C. Wang, Yongjie Wei, Zhenyi An, Zhongju Zou, Guanghua Xiao, Govind Bhagat, Michael White, Julia Reichelt, and Beth Levine (2012) Akt-Mediated Regulation of Autophagy and Tumorigenesis Through Beclin 1 Phosphorylation. Science 338:956-959.

NCBI and Uniprot Product Information

• NCBI GI #: 4502395
• NCBI GeneID: 8678
• NCBI Accession #: NP_003757.1
• NCBI GenBank Nucleotide #: NM_003766.3
• UniProt Accession #: Q14457; O75595; Q9UNA8; B2R6N7
• Molecular Weight: 60
• NCBI Official Full Name: beclin-1
• NCBI Official Synonym Full Names: beclin 1, autophagy related
• NCBI Official Symbol: BECN1
• NCBI Official Synonym Symbols: ATG6; VPS30; beclin1
• NCBI Protein Information: beclin-1; ATG6 autophagy related 6 homolog; coiled-coil myosin-like BCL2-interacting protein; beclin 1 (coiled-coil, moesin-like BCL2 interacting protein); beclin 1 (coiled-coil, moesin-like BCL2-interacting protein)
• UniProt Protein Name: Beclin-1
• Protein Family: Beclin
• UniProt Gene Name: BECN1
• UniProt Synonym Gene Names: GT197
• UniProt Entry Name: BECN1_HUMAN

NCBI Description

Beclin-1 participates in the regulation of autophagy and has an important role in development, tumorigenesis, and neurodegeneration (Zhong et al., 2009 [PubMed 19270693]).[supplied by OMIM, Jul 2010]

Uniprot Description

Function: Plays a central role in autophagy. Required for the abcission step in cytokinesis. May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus. Ref.13

Subunit structure: Interacts with GOPC and GRID2. Interacts with AMBRA1. Forms a complex with PIK3C3 and PIK3R4 and either UVRAG and KIAA0226/Rubicon, or with ATG14. UVRAG and ATG14 form mutually exclusive complexes through direct competition for BECN1-binding. The complex containing ATG14 up-regulates autophagy, while the one containing Rubicon down-regulates autophagy

By similarity. Interacts with BCL2 and BCL2L1. Interacts with VMP1. Interacts with PIK3CB

By similarity. Interacts with DAPK1. Interacts with human cytomegalovirus/HHV-5 protein TRS1. Ref.1 Ref.6 Ref.8 Ref.9 Ref.10 Ref.12 Ref.14 Ref.16

Subcellular location: Golgi apparatus › trans-Golgi network membrane; Peripheral membrane protein. Note: Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells

By similarity. Ref.9

Tissue specificity: Ubiquitous.

Post-translational modification: Phosphorylation at Thr-119 by DAPK1 reduces its interaction with BCL2 and BCL2L1 and promotes induction of autophagy.polyubiquitinated by NEDD4, both with 'Lys11'- and 'Lys63'-linkages. 'Lys'-11-linked poyubiquitination leads to degradation and is enhanced when the stabilizing interaction partner VPS34 is depleted. Deubiquitinated by USP10 and USP13, leading to stabilize the PIK3C3/VPS34-containing complexes.

Sequence similarities: Belongs to the beclin family.