Immunofluorescence (IF)
(MBS9601204 staining HuvEc by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Rabbit anti-Human IL-10R alpha Polyclonal Antibody | anti-IL10RA antibody
Phospho-IL-10R alpha (Tyr496) Antibody
Gene Names
IL10RA; CD210; IL10R; CD210a; CDW210A; HIL-10R; IL-10R1
Reactivity
Human
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Synonyms
IL-10R alpha; Polyclonal Antibody; Phospho-IL-10R alpha (Tyr496) Antibody; CD210; CDw210a; CDw210a antigen; HIL 10R; HIL10R; I10R1_HUMAN; IL 10R 1; IL 10R A; IL 10R; IL 10R1; IL-10 receptor subunit alpha; IL-10R subunit 1; IL-10R subunit alpha; IL-10R1; IL-10RA; IL10R; IL10R1; IL10RA; Interleukin 10 receptor alpha; Interleukin 10 receptor alpha chain; Interleukin-10 receptor subunit 1; Interleukin-10 receptor subunit alpha; anti-IL10RA antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Phospho-IL-10R alpha (Tyr496) antibody detects endogenous levels of IL-10R alpha only when phosphorylated at Tyrosine 496
Purity/Purification
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
578
Applicable Applications for anti-IL10RA antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide derived from human IL-10R alpha around the phosphorylation site of Tyrosine 496
Subcellular Location
Membrane.
Tissue Specificity
Spleen, thymus, and PBMC. Weak expression in pancreas, skeletal muscle, brain, heart, and kidney. Placenta, lung, and liver showed intermediate levels. Monocytes, B-cells, large granular lymphocytes, and T-cells express high levels.
Predicted Cross Reactivity
Pig, Horse, Rabbit, Dog, Chicken
Similarity
Pig (100%), Horse (89%), Rabbit (89%), Dog (100%), Chicken (88%)
Conjugation
Unconjugated
Epitope
Phospho Tyr496
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunofluorescence (IF)
(MBS9601204 staining HuvEc by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescence (IF)
(MBS9601204 staining HuvEc by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Western Blot (WB)
(Western blot analysis of IL-10R alpha phosphorylation expression in HuvEc whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of IL-10R alpha phosphorylation expression in HuvEc whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of Phospho-IL-10R alpha (Tyr496) Antibody expression in HuvEc cells lysates.The lane on the right is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of Phospho-IL-10R alpha (Tyr496) Antibody expression in HuvEc cells lysates.The lane on the right is treated with the antigen-specific peptide.)
Related Product Information for anti-IL10RA antibody
Description: The protein encoded by this gene is a receptor for interleukin 10. This protein is structurally related to interferon receptors. It has been shown to mediate the immunosuppressive signal of interleukin 10, and thus inhibits the synthesis of proinflammatory cytokines.
Function: Receptor for IL10; binds IL10 with a high affinity.
Similarity: Belongs to the type II cytokine receptor family.
Function: Receptor for IL10; binds IL10 with a high affinity.
Similarity: Belongs to the type II cytokine receptor family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 63 kDa
Predicted: 64 kDa
Predicted: 64 kDa
NCBI Official Full Name
interleukin-10 receptor subunit alpha
NCBI Official Synonym Full Names
interleukin 10 receptor subunit alpha
NCBI Official Symbol
IL10RA
NCBI Official Synonym Symbols
CD210; IL10R; CD210a; CDW210A; HIL-10R; IL-10R1
NCBI Protein Information
interleukin-10 receptor subunit alpha
UniProt Protein Name
Interleukin-10 receptor subunit alpha
Protein Family
UniProt Gene Name
IL10RA
UniProt Synonym Gene Names
IL10R; IL-10 receptor subunit alpha; IL-10R subunit alpha; IL-10RA; IL-10R subunit 1; IL-10R1
NCBI Description
The protein encoded by this gene is a receptor for interleukin 10. This protein is structurally related to interferon receptors. It has been shown to mediate the immunosuppressive signal of interleukin 10, and thus inhibits the synthesis of proinflammatory cytokines. This receptor is reported to promote survival of progenitor myeloid cells through the insulin receptor substrate-2/PI 3-kinase/AKT pathway. Activation of this receptor leads to tyrosine phosphorylation of JAK1 and TYK2 kinases. Two transcript variants, one protein-coding and the other not protein-coding, have been found for this gene. [provided by RefSeq, Jan 2009]
Uniprot Description
Receptor for IL10; binds IL10 with a high affinity.
Research Articles on IL10RA
Similar Products
Product Notes
The IL10RA il10ra (Catalog #AAA9601204) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-IL-10R alpha (Tyr496) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IL-10R alpha can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:2000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the IL10RA il10ra for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL-10R alpha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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