Western Blot
(Western blot analysis of extracts from MCF-7 cells, usingPhospho-MFF (Ser172/146) Antibody. Lane1 was treated withphospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)
Rabbit anti-Human MFF Polyclonal Antibody | anti-MFF antibody
Phospho-MFF (Ser172) Antibody
IHC: 1:50-1:200
ELISA: 1:20000 - 1:40000
The optimal dilutions should be determined by the end user.
IMPORTANT: For western blot, incubate membrane with diluted primary Abin 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking,overnight.
Western Blot
(Western blot analysis of extracts from MCF-7 cells, usingPhospho-MFF (Ser172/146) Antibody. Lane1 was treated withphospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)
Western Blot
(Western blot analysis of extracts from MCF-7 cells, usingPhospho-MFF (Ser172/146) Antibody. Lane1 was treated withphospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)
Immunohistochemistry (IHC)
(At 1/100 staining Human esophageal cancer by IHC-P.The sample was formaldehyde fixed and a heat mediatedantigen retrieval step in citrate buffer was performed. Thesample was then blocked and incubated with the primaryantibody at 4°C overnight. An HRP conjugated anti-Rabbitantibody was used as the secondary antibody.)
Immunohistochemistry (IHC)
(At 1/100 staining Human esophageal cancer by IHC-P.The sample was formaldehyde fixed and a heat mediatedantigen retrieval step in citrate buffer was performed. Thesample was then blocked and incubated with the primaryantibody at 4°C overnight. An HRP conjugated anti-Rabbitantibody was used as the secondary antibody.)
NCBI and Uniprot Product Information
NCBI Description
This is a nuclear gene encoding a protein that functions in mitochondrial and peroxisomal fission. The encoded protein recruits dynamin-1-like protein (DNM1L) to mitochondria. There are multiple pseudogenes for this gene on chromosomes 1, 5, and X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013]
Uniprot Description
Plays a role in mitochondrial and peroxisomal fission. Promotes the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface. May be involved in regulation of synaptic vesicle membrane dynamics by recruitment of DNM1L to clathrin-containing vesicles.
Research Articles on MFF
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Product Notes
The MFF mff (Catalog #AAA9600693) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-MFF (Ser172) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MFF can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200 ELISA: 1:20000 - 1:40000 The optimal dilutions should be determined by the end user. IMPORTANT: For western blot, incubate membrane with diluted primary Abin 5% w/v milk, 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking,overnight. Researchers should empirically determine the suitability of the MFF mff for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MFF, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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