Western Blot (WB)
(The Cell and Tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PGP9.5 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Mouse Brain lysate Lane 2.: U87-MG cell lysate )
Mouse anti-Human, Mouse PGP9.5 Monoclonal Antibody | anti-PGP9.5 antibody
PGP9.5 antibody
Gene Names
UCHL1; PGP9.5
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, Immunohistochemistry
Purity
By protein-G affinity chromatography
Synonyms
PGP9.5; Monoclonal Antibody; PGP9.5 antibody; ubiquitin carboxyl-terminal esterase L1; uCHL1; 1-223aa; uCH-L1; EC 3.4.19.12; ubiquitin thioesterase L1; Neuron cytoplasmic protein 9.5; PGP 9.5; ubiquitin carboxyl-terminal hydrolase isozyme L1; ubiquitin carboxyl-terminal esterase L1 Gracile axonal dystrophy; Park 5; Parkinson Disease 5; PGP95; uCH L1; Protein gene product 9.5; ubiquitin C terminal esterase L1; ubiquitin C terminal hydrolase (neuron specific); ubiquitin carboxyl terminal esterase L1; ubiquitin carboxyl terminal hydrolase isozyme L1; ubiquitin thiolesterase; ubiquitin thiolesterase L1.; anti-PGP9.5 antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG2a, k
Clone Number
3D9
Purity/Purification
By protein-G affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
139
Applicable Applications for anti-PGP9.5 antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC)
Application Notes
Recommended dilution range for Western blot is 1:500 ~ 1:2,000 and immunohistochemistry analysis is 1:100~200.
Recommended starting dilution for Western blot is 1:1,000 and Immunohistochemistry is 1:100.
Recommended starting dilution for Western blot is 1:1,000 and Immunohistochemistry is 1:100.
Antigen Species
Human
Immunogen
Recombinant human PGP9.5 (1-223aa) purified from E. coli
Clone Note
Anti-human PGP9.5 mAb, clone 3D9, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PGP9.5 protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Western Blot (WB)
(The Cell and Tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PGP9.5 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Mouse Brain lysate Lane 2.: U87-MG cell lysate )
Western Blot (WB)
(The Cell and Tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PGP9.5 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Mouse Brain lysate Lane 2.: U87-MG cell lysate )
Immunohistochemistry (IHC)
(Paraffin embedded sections of human nerve tissue were incubated with anti-human PGP9.5 (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).)
Immunohistochemistry (IHC)
(Paraffin embedded sections of human nerve tissue were incubated with anti-human PGP9.5 (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).)
Immunofluorescence (IF)
(ICC/IF analysis of PGP9.5 in U87MG cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PGP9.5 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Immunofluorescence (IF)
(ICC/IF analysis of PGP9.5 in U87MG cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PGP9.5 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Related Product Information for anti-PGP9.5 antibody
PGP9.5/UCH-L1 is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. PGP9.5 is a component of the ubiquitin system, which has a fundamental role in regulating various biological activities. PGP9.5 gene encodes two opposing enzymatic activities that affect alpha-synuclein degradation and Parkinson's disease susceptibility.
Product Categories/Family for anti-PGP9.5 antibody
References
kenji H et al, (1999) Am J Pathol 155 (3):711-715; Liu Y et al, (2002) Cell 111 (2):209-18; Mikako Sakurai.et al, (2006) Cell 119:162-171
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
24,888 Da
NCBI Official Full Name
PGP9.5, partial
NCBI Official Symbol
UCHL1
NCBI Official Synonym Symbols
PGP9.5
NCBI Protein Information
ubiquitin carboxyl-terminal hydrolase isozyme L1
UniProt Protein Name
Ubiquitin carboxyl-terminal hydrolase isozyme L1
UniProt Gene Name
UCHL1
UniProt Synonym Gene Names
UCH-L1; PGP9.5
Uniprot Description
Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity ().
Research Articles on PGP9.5
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Product Notes
The PGP9.5 uchl1 (Catalog #AAA200060) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PGP9.5 antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PGP9.5 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC). Recommended dilution range for Western blot is 1:500 ~ 1:2,000 and immunohistochemistry analysis is 1:100~200. Recommended starting dilution for Western blot is 1:1,000 and Immunohistochemistry is 1:100. Researchers should empirically determine the suitability of the PGP9.5 uchl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PGP9.5, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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