OGT recombinant protein

Recombinant OGT protein

Cat. Num. AAA388228

• Gene Names: Ogt; Ogtl; AI115525; 1110038P24Rik; 4831420N21Rik
• Purity: The purity of OGT protein is >90 by SDS-PAGE.
• Synonyms: OGT; Recombinant OGT protein; O-linked N-Acetylglucosamine Transferase; GlcNAc; EC 2.4.1.186 HINCUT-1; O-GLCNAC; HRNT1; OGT recombinant protein

• Host: Baculovirus
• Purity/Purification: The purity of OGT protein is >90 by SDS-PAGE.
• Form/Format: Recombinant full length Mouse OGT protein is supplied in 25mM HEPES pH7.5, 300mM NaCl, 5% glycerol, 0.04% Triton X-100, 0.2mM TCEP.

• Protein Species: Mouse
• Tag: DYKDDDDK-Tag
• Notes: This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant OGT protein gel. OGT protein was run on an 8% SDS-PAGE gel and stained with Coomassie Blue.)

Related Product Information for OGT recombinant protein

Short Description: Recombinant mouse OGT was expressed in a baculovirus expression system as the full-length protein (accession number NP_001277464.1) with an N-terminal DYKDDDDK-Tag. The molecular weight of the protein is 118.7 kDa. The purity of OGT protein is >90 by SDS-PAGE. It is suitable for the study of serine/threonine glycosylation, regulation of H3K4 methylation and gene expression.

Background: OGT (O-linked N-acetylglucosamine (GlcNAc) transferase) catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to serine or threonine residues in cytoplasmic and nuclear proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc). OGT can glycosylate a large and diverse number of proteins including HCF1, AKT1, MLL5 and histone H2B. It can regulate their cellular processes via cross-talk between glycosylation and phosphorylation or by affecting proteolytic processing. Specifically OGT has been shown to interact directly with TET2 and TET3, enzymes that catalyze the oxidation of 5-methylcytosine on DNA. The TET/OGT interaction leads to GlcNAcetylation of HCF1, a protein component of the COMPASS complex, which regulates H3K4 methylation and gene expression.

Product Categories/Family for OGT recombinant protein

Recombinant Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 594542444
• NCBI GeneID: 108155
• NCBI Accession #: NP_001277464.1
• NCBI GenBank Nucleotide #: NM_001290535.1
• UniProt Accession #: Q8CGY8; Q6PG10; Q8BXH6; Q91Y38; A2ALY1
• Molecular Weight: 116,952 Da
• NCBI Official Full Name: UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit isoform 2
• NCBI Official Synonym Full Names: O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase)
• NCBI Official Symbol: Ogt
• NCBI Official Synonym Symbols: Ogtl; AI115525; 1110038P24Rik; 4831420N21Rik
• NCBI Protein Information: UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit; O-GlcNAc transferase subunit p110; O-linked N-acetylglucosamine transferase 110 kDa subunit
• UniProt Protein Name: UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit
• Protein Family: Methylated-DNA--protein-cysteine methyltransferase
• UniProt Gene Name: Ogt
• UniProt Synonym Gene Names: OGT
• UniProt Entry Name: OGT1_MOUSE