WTAP recombinant protein

Recombinant WTAP protein

Cat. Num. AAA388273

• Gene Names: WTAP; Mum2
• Synonyms: WTAP; Recombinant WTAP protein; Wilms Tumor 1 Associated Protein; WT1-Associated Protein; WTAP recombinant protein

• Host: Baculovirus
• Form/Format: Recombinant WTAP protein is supplied at a concentration of 0.2ug/ul in 25mM HEPES pH7.5, 300mM NaCl, 5% glycerol, 0.04% Triton X-100, 0.2mM TCEP.

• Protein Species: Human
• Tag: DYKDDDDK-Tag
• Notes: This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant WTAP protein gel. Recombinant WTAP protein was run on an 8% SDS-PAGE gel and stained with Coomassie blue.)

Related Product Information for WTAP recombinant protein

Short Description: Recombinant WTAP protein was expressed in a baculovirus expression system as the full-length protein (accession number NP_004897.2) with an N-terminal DYKDDDDK-Tag. The molecular weight of WTAP is 45.2 kDa. It is suitable for use in functional assays and analysis of transcription regulation in vitro.

Background: N6-methylated adenine (m6A) is prevalently present in nearly all RNA types and can be found in all organisms from bacteria to humans. It preferentially appears around stop codons and within long internal exons in mammalian messager RNAs. m6A plays an important role in the efficiency of mRNA splicing, processing, translation efficiency, editing and mRNA stability. m6A also takes place in other RNA molecules, such as primary miRNA (pri-miRNAs). WTAP (Wilms Tumor 1 Associated Protein, as known as Mum2) is a ubiquitously expressed nuclear protein. WTAP is the regulatory subunit of the WMM N6-methyltransferase complex, a multiprotein complex that mediates N6-methyladenosine (m6A) methylation of some adenosine residues of some mRNAs. WTAP is required for accumulation of METTL3 and METTL14 to nuclear speckle. It acts as a mRNA splicing regulator. WTAP also regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability. WTAP can impair WT1 DNA-binding ability and inhibit expression of WT1 target genes.

Product Categories/Family for WTAP recombinant protein

Recombinant Proteins; Transcription

NCBI and Uniprot Product Information

• NCBI GI #: 21361159
• NCBI GeneID: 9589
• NCBI Accession #: NP_004897.2
• NCBI GenBank Nucleotide #: NM_004906.4
• UniProt Accession #: Q15007; Q5TCL8; Q5TCL9; Q96T28; Q9BYJ7; Q9H4E2
• Molecular Weight: 17,801 Da
• NCBI Official Full Name: pre-mRNA-splicing regulator WTAP isoform 1
• NCBI Official Synonym Full Names: Wilms tumor 1 associated protein
• NCBI Official Symbol: WTAP
• NCBI Official Synonym Symbols: Mum2
• NCBI Protein Information: pre-mRNA-splicing regulator WTAP
• UniProt Protein Name: Pre-mRNA-splicing regulator WTAP
• Protein Family: Pre-mRNA-splicing regulator
• UniProt Gene Name: WTAP
• UniProt Synonym Gene Names: KIAA0105; hFL(2)D
• UniProt Entry Name: FL2D_HUMAN

NCBI Description

The Wilms tumor suppressor gene WT1 appears to play a role in both transcriptional and posttranscriptional regulation of certain cellular genes. This gene encodes a WT1-associating protein, which is a ubiquitously expressed nuclear protein. Like WT1 protein, this protein is localized throughout the nucleoplasm as well as in speckles and partially colocalizes with splicing factors. Alternative splicing of this gene results in several transcript variants encoding three different isoforms. [provided by RefSeq, Jul 2012]

Uniprot Description

WTAP: Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability. Impairs WT1 DNA- binding ability and inhibits expression of WT1 target genes. May be involved in mRNA splicing regulation. Belongs to the fl(2)d family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleolus; Spliceosome

Chromosomal Location of Human Ortholog: 6q25-q27

Cellular Component: nucleoplasm; nuclear membrane; nucleolus; nuclear speck; nucleus

Biological Process: RNA splicing; cell cycle; mRNA processing; regulation of alternative nuclear mRNA splicing, via spliceosome