PELI1 recombinant protein

Recombinant PELI1 protein

Cat. Num. AAA389358

• Synonyms: PELI1; Recombinant PELI1 protein; E3 ubiquitin-protein ligase pellino homolog 1 (Peli1); PELI1 recombinant protein

• Host: Baculovirus
• Form/Format: Recombinant PELI1 protein is supplied in 25mM HEPES-NaOH pH7.5, 300mM NaCl, 10% glycerol, 0.04% Triton X-100, and 0.5mM TCEP.

• Protein Species: Human
• Tag: DYKDDDDK-Tag
• Notes: This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant PELI1 protein gel 10% SDS-PAGE with Coomassie blue staining MW: 46.3 kDa Purity: >90%)

Related Product Information for PELI1 recombinant protein

Short Description: Full-length human PELI1 was expressed in a baculovirus expression system (accession number NP_065702.2) with an N-terminal DYKDDDDK-Tag. The molecular weight of the protein is 46.3 kDa.

Background: PELI1 (Pellino E3 Ubiquitin Protein Ligase 1), also called as Pellino-1 or PRISM, is a E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. It is involved in the TLR and IL-1 signaling pathways via interaction with the complex containing IRAK kinases and TRAF6. Besides mediating 'Lys-63'-linked polyubiquitination of IRAK1 allowing subsequent NF-kappa-B activation, PELI1 also is reported to Mediate 'Lys-48'-linked polyubiquitination of RIPK3 leading to its subsequent proteasome-dependent degradation. It preferentially recognizes and mediates the degradation of the 'Thr-182' phosphorylated form of RIPK3 and negatively regulates necroptosis by reducing RIPK3 expression.

Product Categories/Family for PELI1 recombinant protein

Recombinant Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 11037063
• NCBI GeneID: 57162
• NCBI Accession #: NP_065702.2
• NCBI GenBank Nucleotide #: NM_020651.3
• UniProt Accession #: Q96FA3; Q96SM0; Q9GZY5; Q9HCX0
• Molecular Weight: 46,286 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase pellino homolog 1
• NCBI Official Synonym Full Names: pellino E3 ubiquitin protein ligase 1
• NCBI Official Symbol: PELI1
• NCBI Protein Information: E3 ubiquitin-protein ligase pellino homolog 1; pellino-1; protein pellino homolog 1; pellino-related intracellular-signaling molecule
• UniProt Protein Name: E3 ubiquitin-protein ligase pellino homolog 1
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: PELI1
• UniProt Synonym Gene Names: PRISM; Pellino-1
• UniProt Entry Name: PELI1_HUMAN

Uniprot Description

PELI1: E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. Involved in the TLR and IL-1 signaling pathways via interaction with the complex containing IRAK kinases and TRAF6. Mediates 'Lys-63'-linked polyubiquitination of IRAK1 allowing subsequent NF-kappa-B activation. Found in a complex containing TRAF6, IRAK1 and IRAK4. Interacts with MAP3K7. Belongs to the pellino family.

Protein type: EC 6.3.2.-; EC 6.3.2.19; Adaptor/scaffold; Ubiquitin conjugating system; Ligase; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 2p13.3

Cellular Component: cytosol; nucleus

Molecular Function: protein binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: positive regulation of cytokine production; positive regulation of toll-like receptor 3 signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB cascade; response to dsRNA; positive regulation of toll-like receptor 4 signaling pathway; response to lipopolysaccharide; Toll signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; negative regulation of T cell proliferation; positive regulation of protein ubiquitination; inhibition of NF-kappaB transcription factor; positive regulation of B cell proliferation; toll-like receptor signaling pathway; innate immune response; toll-like receptor 9 signaling pathway; toll-like receptor 4 signaling pathway