IRF3 recombinant protein

Recombinant IRF3 protein

Cat. Num. AAA388248

• Purity: The IRF3 protein is >90% pure by SDS-PAGE.
• Synonyms: IRF3; Recombinant IRF3 protein; Interferon Regulatory Factor 3; IRF-3; IRF3 recombinant protein

• Host: Baculovirus
• Purity/Purification: The IRF3 protein is >90% pure by SDS-PAGE.
• Form/Format: Recombinant full length IRF3 protein is supplied in 25mM HEPES pH7.5, 300mM NaCl, 5% glycerol, 0.04% Triton X-100, 0.2mM TCEP.

• Protein Species: Human
• Tag: DYKDDDDK-Tag
• Notes: Recombinant IRF3 Protein is useful for the study of protein-protein interaction, screening inhibitors, and selectivity profiling. This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant IRF3 protein gel.IRF3 protein was run on a 10% SDS-PAGE gel and stained with Coomassie Blue.)

Related Product Information for IRF3 recombinant protein

Short Description: Recombinant human IRF3 was expressed in a baculovirus expression system as the full-length protein (accession number NP_001562.1) with an N-terminal DYKDDDDK-Tag. The molecular weight of the protein is of 50.2 kDa. The IRF3 protein is >90% pure by SDS-PAGE. It is suitable for the study of protein-protein interactions, inhibitor screening, and selectivity profiling.

Background: IRF3 (Interferon regulatory factor 3)is a member of the IRF family of transcription factors that include IRF1, IRF6 and IRF7. IRF3 that has been phosphorylated at serine/threonine forms a complex with CREBBP and translocates to the nucleus to activate transcription of interferon-induced genes. IRF3 plays a critical role in the innate immune response against DNA and RNA viruses.

Product Categories/Family for IRF3 recombinant protein

Recombinant Proteins; Transcription

NCBI and Uniprot Product Information

• NCBI GI #: 4504725
• NCBI GeneID: 3661
• NCBI Accession #: NP_001562.1
• NCBI GenBank Nucleotide #: NM_001571.5
• UniProt Accession #: Q14653; Q5FBY1; Q5FBY2; Q5FBY4; Q7Z5G6; A8K7L2; B2RAZ3
• Molecular Weight: 12,330 Da
• NCBI Official Full Name: interferon regulatory factor 3 isoform 1
• NCBI Official Synonym Full Names: interferon regulatory factor 3
• NCBI Official Symbol: IRF3
• NCBI Protein Information: interferon regulatory factor 3
• UniProt Protein Name: Interferon regulatory factor 3
• Protein Family: Interferon regulatory factor
• UniProt Gene Name: IRF3
• UniProt Synonym Gene Names: IRF-3
• UniProt Entry Name: IRF3_HUMAN

NCBI Description

This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]

Uniprot Description

IRF3: interferon regulatory factor 3, a member of the interferon regulatory transcription factor (IRF) family. IRF3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 19q13.3-q13.4

Cellular Component: nucleoplasm; cytoplasm; cytosol; nucleus

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; DNA binding; transcription cofactor activity; transcription factor activity

Biological Process: negative regulation of interferon-beta biosynthetic process; transcription from RNA polymerase II promoter; positive regulation of I-kappaB kinase/NF-kappaB cascade; viral reproduction; apoptosis; cytokine and chemokine mediated signaling pathway; MyD88-independent toll-like receptor signaling pathway; negative regulation of transcription from RNA polymerase II promoter; toll-like receptor 3 signaling pathway; negative regulation of defense response to virus by host; response to exogenous dsRNA; positive regulation of interferon type I production; lipopolysaccharide-mediated signaling pathway; positive regulation of interferon-beta production; toll-like receptor signaling pathway; innate immune response; interferon type I biosynthetic process; toll-like receptor 4 signaling pathway; response to DNA damage stimulus; negative regulation of interferon type I production; defense response to virus; positive regulation of interferon-alpha production; positive regulation of cytokine secretion

Disease: Herpes Simplex Encephalitis, Susceptibility To, 7