Testing Data
Oxidized Protein Immunoblot Control (Carbonyl-BSA) Native Protein
Oxidized Protein Immunoblot Control (Carbonyl-BSA)
Synonyms
Oxidized Protein Immunoblot Control (Carbonyl-BSA); Oxidized Protein Immunoblot Control (Carbonyl-BSA) native protein
Methods
Dilute the Carbonyl-BSA control with reducing SDS-PAGE sample buffer to 5 ug/mL and heat for 5 min in boiling water. Load 20 uL per lane for western blot analysis of protein carbonyl using MyBioSource's Protein Carbonyl Immunoblot Kit (STA-308) or anti-DNP antibody after DNPH deverization.
Quantity
200 uL of 50 ug/mL Carbonyl-BSA in 1X PBS
Preparation and Storage
-20 degree C. Shelf Life: 1 year from receipt under proper storage conditions; aliquot to avoid multiple freeze thaw cycles
Testing Data
Testing Data
Related Product Information for Oxidized Protein Immunoblot Control (Carbonyl-BSA) native protein
• Provided at 50 ug/mL
Protein oxidation is defined as the covalent modification of a protein induced either directly by reactive oxygen species or indirectly by reaction with secondary by-products of oxidative stress. Oxidative modification of proteins can be induced in vitro by a wide array of pro-oxidant agents and occurs in vivo during aging and in certain disease conditions.
There are numerous types of protein oxidative modifications. The most common products of protein oxidation in biological samples are the protein carbonyl derivatives of Pro, Arg, Lys, and Thr. These derivatives are chemically stable and serve as markers of oxidative stress for most types of ROS. Many of the assays involve derivitization of the carbonyl group with dinitrophenylhydrazine (DNPH), which leads to formation of a stable dinitrophenyl hydrazone product, followed by imuunoblooting with anti-DNP antibody.
Protein oxidation is defined as the covalent modification of a protein induced either directly by reactive oxygen species or indirectly by reaction with secondary by-products of oxidative stress. Oxidative modification of proteins can be induced in vitro by a wide array of pro-oxidant agents and occurs in vivo during aging and in certain disease conditions.
There are numerous types of protein oxidative modifications. The most common products of protein oxidation in biological samples are the protein carbonyl derivatives of Pro, Arg, Lys, and Thr. These derivatives are chemically stable and serve as markers of oxidative stress for most types of ROS. Many of the assays involve derivitization of the carbonyl group with dinitrophenylhydrazine (DNPH), which leads to formation of a stable dinitrophenyl hydrazone product, followed by imuunoblooting with anti-DNP antibody.
Product Categories/Family for Oxidized Protein Immunoblot Control (Carbonyl-BSA) native protein
References
1. Cadenas, E., Boveris, A., Ragan, CI., and Stoppani, AO. (1977). Archives of Biochemistry & Biophysics 248257.
2. Wakeyama, H., Takeshige, K., Takayanagi, R., and Minakami, S. (1982). Biochem J 593601.
3. Talent, JM., Kong, Y., and Gracy, RW. (1998). Anal.Biochem. 3138.
Biswas, C. et al. (2014). Nuclear Heme Oxygenase-1 (HO-1) Modulates Subcellular Distribution and Activation of Nrf2, Impacting Metabolic and Anti-oxidant Defenses. J Biol Chem. 289:26882-26894.
2. Wakeyama, H., Takeshige, K., Takayanagi, R., and Minakami, S. (1982). Biochem J 593601.
3. Talent, JM., Kong, Y., and Gracy, RW. (1998). Anal.Biochem. 3138.
Biswas, C. et al. (2014). Nuclear Heme Oxygenase-1 (HO-1) Modulates Subcellular Distribution and Activation of Nrf2, Impacting Metabolic and Anti-oxidant Defenses. J Biol Chem. 289:26882-26894.
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Product Notes
The Oxidized Protein Immunoblot Control (Carbonyl-BSA) (Catalog #AAA168896) is a Native Protein and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Oxidized Protein Immunoblot Control (Carbonyl-BSA), Native Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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