Western Blot (WB)
(Western blotAll lanes: EIF3K antibody at 1.72ug/mlLane 1: Thp-1 whole cell lysateLane 2: HT29 whole cell lysateLane 3: 293T whole cell lysateLane 4: Hela whole cell lysateSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 26,25 kDaObserved band size: 25 kDa)
Rabbit anti-Human EIF3K Polyclonal Antibody | anti-EIF3K antibody
EIF3K Antibody
Western Blot (WB)
(Western blotAll lanes: EIF3K antibody at 1.72ug/mlLane 1: Thp-1 whole cell lysateLane 2: HT29 whole cell lysateLane 3: 293T whole cell lysateLane 4: Hela whole cell lysateSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 26,25 kDaObserved band size: 25 kDa)
Western Blot (WB)
(Western blotAll lanes: EIF3K antibody at 1.72ug/mlLane 1: Thp-1 whole cell lysateLane 2: HT29 whole cell lysateLane 3: 293T whole cell lysateLane 4: Hela whole cell lysateSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 26,25 kDaObserved band size: 25 kDa)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human liver cancer using MBS7045840 at dilution 1:100)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human liver cancer using MBS7045840 at dilution 1:100)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human kidney tissue using MBS7045840 at dilution 1:100)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human kidney tissue using MBS7045840 at dilution 1:100)
Immunoprecipitation (IP)
(Immunoprecipitating EIF3K in HeLa whole cell lysateLane 1: Rabbit monoclonal IgG(1ug)instead of MBS7045840 in HeLa whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)Lane 2: MBS7045840(4ug)+ HeLa whole cell lysate(500ug)Lane 3: HeLa whole cell lysate (20ug))
Immunoprecipitation (IP)
(Immunoprecipitating EIF3K in HeLa whole cell lysateLane 1: Rabbit monoclonal IgG(1ug)instead of MBS7045840 in HeLa whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)Lane 2: MBS7045840(4ug)+ HeLa whole cell lysate(500ug)Lane 3: HeLa whole cell lysate (20ug))
NCBI and Uniprot Product Information
NCBI Description
The 700-kD eukaryotic translation initiation factor-3 (eIF3) is the largest eIF and contains at least 12 subunits, including EIF2S12. eIF3 plays an essential role in translation by binding directly to the 40S ribosomal subunit and promoting formation of the 40S preinitiation complex (Mayeur et al., 2003 [PubMed 14519125]).[supplied by OMIM, Mar 2008]
Uniprot Description
EIF3K: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Belongs to the eIF-3 subunit K family.
Protein type: Translation initiation; Translation
Chromosomal Location of Human Ortholog: 19q13.2
Cellular Component: cytoplasm; cytosol; eukaryotic translation initiation factor 3 complex; membrane
Molecular Function: protein binding; translation initiation factor activity
Biological Process: translational initiation
Research Articles on EIF3K
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Product Notes
The EIF3K eif3k (Catalog #AAA7045840) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EIF3K Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EIF3K can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the EIF3K eif3k for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EIF3K, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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