Goat anti-Any N-epsilon-CML Polyclonal Antibody

Goat Anti-N-epsilon-CML Polyclonal Antibody

Cat. Num. AAA169045

• Reactivity: Any
• Applications: Immunoblot, Western Blot, ELISA
• Purity: Affinity Purified
• Synonyms: N-epsilon-CML; Polyclonal Antibody; Goat Anti-N-epsilon-CML Polyclonal Antibody; anti-N-epsilon-CML antibody

• Host: Goat
• Reactivity: Any
• Clonality: Polyclonal
• Specificity: CML-modified proteins
• Purity/Purification: Affinity Purified
• Form/Format: 100 ug of affinity purified antibody at 0.82 mg/mL in 75 mM PBS, pH 7.2, containing 0.5 mM EDTA and 0.02% NaN3

• Applicable Applications for anti-N-epsilon-CML antibody: Immunoblot (IB), ELISA (EIA)
• Application Notes: Immunoblot (1:200 to 1:20,000); ELISA (1:200 to 1:20,000)
• Immunogen: CML-KLH
• Conjugation: Unconjugated
• Quantity: 100 ug of affinity purified antibody at 0.82 mg/mL in 75 mM PBS, pH 7.2, containing 0.5 mM EDTA and 0.02% NaN3; Note: slight precipitation in the tube is normal; centrifuge before use
• Preparation and Storage: -20 degree C. Shelf Life: 1 year from date of receipt under proper storage conditions; aliquot to avoid multiple freeze thaw cycles

Testing Data

Related Product Information for anti-N-epsilon-CML antibody

• Affinity purified

The non-enzymatic reaction of reducing carbohydrates with lysine side chains and N-terminal amino groups of macromolecules (proteins, phospholipids and nucleic acids) is called the Maillard reaction or glycation. The products of this process, termed advanced glycation end products (AGEs), adversely affect the functional properties of proteins, lipids and DNA. Tissue levels of AGE increase with age and the formation of AGEs is predominantly endogenous, though these products can also be derived from exogenous sources such as food and tobacco smoke. AGE modification of proteins can contribute to the pathophysiology of aging and long-term complications of diabetes, atherosclerosis and renal failure. AGEs also interact with a variety of cell-surface AGE-binding receptors (RAGE), leading either to their endocytosis and degradation or to cellular activation and pro-oxidant or pro-inflammatory events.

Although several AGE structures have been reported, it was demonstrated that Nepsilon-(carboxymethyl) lysine (CML) is a major antigenic AGE structure. CML concentration is increased in patients who have diabetes with complications, including nephropathy, retinopathy, and atherosclerosis. CML is also recognized by receptor for AGE (RAGE), and CML-RAGE interaction activates cell signaling pathways such as NF-kappaB.

Product Categories/Family for anti-N-epsilon-CML antibody

Oxidative Stress/Damage; Protein Oxidation/Nitration; Advanced Glycation End Products; Antibodies against Advanced Glycation End Products