Rabbit anti-Human FGR Antibody | anti-FGR antibody

FGR Antibody

Cat. Num. AAA8503304

• Gene Names: FGR; SRC2; c-fgr; c-src2; p55-Fgr; p58-Fgr; p55c-fgr; p58c-fgr
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity purification
• Synonyms: FGR; FGR Antibody; SRC2; c-fgr; c-src2; p55-Fgr; p55c-fgr; p58-Fgr; p58c-fgr; anti-FGR antibody

• Host: Rabbit
• Reactivity: Human
• Isotype: IgG
• Purity/Purification: Affinity purification
• Sequence Length: 529

• Applicable Applications for anti-FGR antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: A synthetic peptide of human FGR
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.

Testing Data

(Western blot analysis of extracts of HL60cell lines, using FGR antibody.)

Related Product Information for anti-FGR antibody

Fgr is a member of the Src tyrosine kinase family. It has a membrane-associated amino-terminal domain that is highly divergent from other family members, internal conserved SH2 and SH3 domains and a highly conserved carboxy-terminal tyrosine kinase catalytic domain (1, 2). Tyrosine 412 is located in the activation loop, and phosphorylation of this residue is critical for the activation of Fgr tyrosine kinase activity. c-Fgr is predominantly expressed in cells of hematopoietic origin including differentiated myeloid cells, NK and B cells (3, 4). Fgr plays an important role in the signaling cascade from membrane receptors lacking intrinsic tyrosine kinase activity such as Bcr, FcR, and the integrin family of receptors (5). It was demonstrated that Fgr functions as a selective inhibitor of beta2 integrin-mediated signaling and Syk kinase function in monocytes (5).

Product Categories/Family for anti-FGR antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

1. Willman, C.L. et al. (1991) Blood 77, 726-734. 2. Patel, M. et al. (1991) Pathobiology 59, 289-292. 3. Wechsler, R.J. and Monroe, J.G. (1995) J. Immunol. 154, 3234-3244. 4. Notario, V. et al. (1989) J. Cell Biol. 109, 3129-3136. 5. Vines, C.M. et al. (2001) Immunity 15, 507-519.

NCBI and Uniprot Product Information

• NCBI GI #: 112382241
• NCBI GeneID: 2268
• NCBI Accession #: NP_001036194.1
• NCBI GenBank Nucleotide #: NM_001042729.1
• UniProt Accession #: P09769; Q9UIQ3; D3DPL7
• Molecular Weight: 56kDa
• NCBI Official Full Name: tyrosine-protein kinase Fgr
• NCBI Official Synonym Full Names: FGR proto-oncogene, Src family tyrosine kinase
• NCBI Official Symbol: FGR
• NCBI Official Synonym Symbols: SRC2; c-fgr; c-src2; p55-Fgr; p58-Fgr; p55c-fgr; p58c-fgr
• NCBI Protein Information: tyrosine-protein kinase Fgr
• UniProt Protein Name: Tyrosine-protein kinase Fgr
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: FGR
• UniProt Synonym Gene Names: SRC2

NCBI Description

This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to plasma membrane ruffles, and functions as a negative regulator of cell migration and adhesion triggered by the beta-2 integrin signal transduction pathway. Infection with Epstein-Barr virus results in the overexpression of this gene. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

Non-receptor tyrosine-protein kinase that transmits signals from cell surface receptors devoid of kinase activity and contributes to the regulation of immune responses, including neutrophil, monocyte, macrophage and mast cell functions, cytoskeleton remodeling in response to extracellular stimuli, phagocytosis, cell adhesion and migration. Promotes mast cell degranulation, release of inflammatory cytokines and IgE-mediated anaphylaxis. Acts downstream of receptors that bind the Fc region of immunoglobulins, such as MS4A2/FCER1B, FCGR2A and/or FCGR2B. Acts downstream of ITGB1 and ITGB2, and regulates actin cytoskeleton reorganization, cell spreading and adhesion. Depending on the context, activates or inhibits cellular responses. Functions as negative regulator of ITGB2 signaling, phagocytosis and SYK activity in monocytes. Required for normal ITGB1 and ITGB2 signaling, normal cell spreading and adhesion in neutrophils and macrophages. Functions as positive regulator of cell migration and regulates cytoskeleton reorganization via RAC1 activation. Phosphorylates SYK (in vitro) and promotes SYK-dependent activation of AKT1 and MAP kinase signaling. Phosphorylates PLD2 in antigen-stimulated mast cells, leading to PLD2 activation and the production of the signaling molecules lysophosphatidic acid and diacylglycerol. Promotes activation of PIK3R1. Phosphorylates FASLG, and thereby regulates its ubiquitination and subsequent internalization. Phosphorylates ABL1. Promotes phosphorylation of CBL, CTTN, PIK3R1, PTK2/FAK1, PTK2B/PYK2 and VAV2. Phosphorylates HCLS1 that has already been phosphorylated by SYK, but not unphosphorylated HCLS1.