Mouse MIP-2 CLIA Kit | MIP-2 clia kit

MIP-2 (Murine) LumiAb ELISA Kit

Cat. Num. AAA9510851

• Gene Names: Cxcl2; GROb; Gro2; Mip2; Scyb; MIP-2; Scyb2; MIP-2a; Mgsa-b; CINC-2a
• Reactivity: Mouse
• Synonyms: MIP-2; MIP-2 (Murine) LumiAb ELISA Kit; Murine MIP-2; C-X-C motif chemLumine 2; Macrophage inflammatory protein 2; Scyb2; MIP-2 clia kit

• Reactivity: Mouse
• Specificity: The LumiAb Murine MDC ELISA is capable of recognizing both recombinant and naturally produced Murine MDC proteins. The antigens listed below were tested at 50 ng/mL and did not exhibit significant cross reactivity or interference.
• Sequence Length: 100

• Assay Type: Quantitative Sandwich
• Sensitivity: 4 pg/ml.
• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MIP-2 clia kit

Principle of the Assay: The LumiAb Murine MDC Chemiluminescent ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Murine MDC concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Murine MDC while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a sensitive luminescent reaction to ensue upon substrate addition. When the Peroxide Enhancer solution is added, the reaction catalyzed by peroxidase yields light that is representative of the antigen concentration. After a brief incubation, the microplate can be read with a luminometer, allowing for generation of a standard curve and subsequent determination of protein concentration!!Background/Introduction: Murine MDC, also known as mouse macrophage-derived chemokine, ABCD-1 or CCL22, is a 7.8 kDa signaling protein produced in B lymphocytes (B-cells), microglia, monocyte-derived dendritic cells, activated cytotoxic lymphocytes (NK, natural killer cells), CD4 T lymphocytes (T-cells) and many more cell types. This protein, expressed from the gene Ccl22, is known to be a member of the C-C or beta-chemokine family through its intrinsic pair of disulfide linkages formed by four conserved cysteine residues. In addition, the protein also consists of a C-terminal heparin-binding motif and no potential N-linked glycosylation sites. The precursor mMDC protein is initially synthesized as a 92 amino acid polypeptide characterized by an N-terminal signal sequence followed by the actual mMDC polypeptide. After proteolytic cleavage of the 24 amino acid residue signal sequence, the 68 residue mMDC peptide is allowed to fold into a mature mMDC protein where it can provide support to a plethora of immune functions. Normally, chemokines undergo significant N-terminal processing which allows for up-and down-regulation of their physiology and function. Moreover, some chemokines are subject to processing by peptidases, considerably reducing their activity on neighboring lymphocytes and monocyte-derived dendritic cells. The MDC protein is implicated in a variety of pathways regarding lymphocyte migration. Through its high affinity binding with CCR4 receptors studded on the plasma membranes of activated T cells, the MDCs expressed by dendritic cells are able to chemoattract these lymphocytes to augment immune functions. In addition, MDCs expressed by dendritic cells have high affinity for and can chemically attract CD4+, CD25+ and CTLA4+ regulatory T cells-all displaying membrane CCR4 receptors-to inflammatory regions where T cells have weakened activation. Another type of MDC produced from B lymphocytes can help stimulate B-T cell interactions through the formation of germinal centers, or sites within lymph nodes that provide a hub for B-cell proliferation, differentiation and hyper-mutation through activation by T-cell dependent antigens. Furthermore, countless studies have revealed that the MDC protein can function as a powerful chemoattractant for dendritic cells, hematopoietic precursor cells and activated cytotoxic killer cells, as well as activate platelet functions and inhibit hippocampal synapses.

Product Categories/Family for MIP-2 clia kit

Luminometer Sandwich ELISA

NCBI and Uniprot Product Information

• NCBI GI #: 6677885
• NCBI GeneID: 20310
• NCBI Accession #: NP_033166.1
• NCBI GenBank Nucleotide #: NM_009140.2
• UniProt Accession #: P10889
• NCBI Official Full Name: C-X-C motif chemokine 2
• NCBI Official Synonym Full Names: chemokine (C-X-C motif) ligand 2
• NCBI Official Symbol: Cxcl2
• NCBI Official Synonym Symbols: GROb; Gro2; Mip2; Scyb; MIP-2; Scyb2; MIP-2a; Mgsa-b; CINC-2a
• NCBI Protein Information: C-X-C motif chemokine 2
• UniProt Protein Name: C-X-C motif chemokine 2
• UniProt Gene Name: Cxcl2
• UniProt Synonym Gene Names: Mip-2; Mip2; Scyb2; MIP2
• UniProt Entry Name: CXCL2_MOUSE

Uniprot Description

Chemotactic for human polymorphonuclear leukocytes but does not induce chemokinesis or an oxidative burst.