Chromatin Immunoprecipitation (ChIP)
(AbFlex TET1 antibody tested by ChIP-Seq. ChIP was performed using AbFlex TET1 recombinant antibody with 30 ug chromatin from human brain tumor cells and 4 ul of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 13 million sequence tags were mapped to identify TET1 binding sites. The image shows binding across a region of chromosome 1.)
Mouse anti-Human, Mouse TET1 Antibody | anti-TET1 antibody
AbFlex TET1 antibody (rAb)
Reactivity
Human, Mouse
Applications
Chromatin Immunoprecipitation, Immunoprecipitation
Purity
Protein A Chromatography
Synonyms
TET1; AbFlex TET1 antibody (rAb); LCX; Cxxc6; AA517754; BB001228; mKIAA1676; D10Ertd17e; 2510010B09Rik; anti-TET1 antibody
Host
Mouse
Reactivity
Human, Mouse
Isotype
IgG2a
Purity/Purification
Protein A Chromatography
Form/Format
Purified IgG in 140mM Hepes, pH7.5, 70mM NaCl, 32mM NaOAc, 0.035% sodium azide, 30% glycerol. Sodium azide is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-TET1 antibody
Chromatin Immunoprecipitation-Seq (ChIP-Seq)
Application Notes
Applications Validated:
ChIP-Seq: 4ul per ChIP
AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone
ChIP-Seq: 4ul per ChIP
AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone
Antibody Type
Recombinant Antibody
Immunogen
This antibody was raised against a recombinant protein comprising amino acids 1682-1914 of mouse TET1 (Ten-Eleven Translocation-1; methylcytosine dioxygenase).
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Chromatin Immunoprecipitation (ChIP)
(AbFlex TET1 antibody tested by ChIP-Seq. ChIP was performed using AbFlex TET1 recombinant antibody with 30 ug chromatin from human brain tumor cells and 4 ul of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 13 million sequence tags were mapped to identify TET1 binding sites. The image shows binding across a region of chromosome 1.)
Chromatin Immunoprecipitation (ChIP)
(AbFlex TET1 antibody tested by ChIP-Seq. ChIP was performed using AbFlex TET1 recombinant antibody with 30 ug chromatin from human brain tumor cells and 4 ul of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 13 million sequence tags were mapped to identify TET1 binding sites. The image shows binding across a region of chromosome 1.)
Western Blot (WB)
(Western blot of AbFlex TET1 antibody. 20 ug nuclear lysate from F9 (mouse testis embryonal carcinoma) cells was probed by Western Blot using 2 ug/mL of antibody.)
Western Blot (WB)
(Western blot of AbFlex TET1 antibody. 20 ug nuclear lysate from F9 (mouse testis embryonal carcinoma) cells was probed by Western Blot using 2 ug/mL of antibody.)
Related Product Information for anti-TET1 antibody
Short Description: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. Our AbFlex TET1 antibody (rAb) was raised in a Mouse host. It has been validated for use in ChIP-Seq; it has been shown to react with Human and Mouse samples.
Background: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex TET1 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. TET1(Ten-Eleven Translocation-1; methylcytosine dioxygenase) is a protein that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) which can lead to cytosine demethylation by either further oxidation via TET proteins to 5-formylcytosine (5fC) and 5-carboxycytosine (5caC) or deamination into 5-hydroxymethyluracil (5hmU) and then subsequent replacement by unmethylated cytosine by the base excision repair system. Methylation at the C5 position of cytosine bases is an epigenetic modification of the mammalian genome which plays an important role in transcriptional regulation. TET1 preferentially binds to CpG-rich sequences at promoters of both transcriptionally active and polycomb-repressed genes. By controlling the levels of 5mC and 5hmC at gene promoters, it may regulate the gene expression silencing induced by cytosine methylation. May have a dual function by also repressing the expression of a subset of genes through recruitment of transcriptional repressors to promoters.
Background: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex TET1 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. TET1(Ten-Eleven Translocation-1; methylcytosine dioxygenase) is a protein that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) which can lead to cytosine demethylation by either further oxidation via TET proteins to 5-formylcytosine (5fC) and 5-carboxycytosine (5caC) or deamination into 5-hydroxymethyluracil (5hmU) and then subsequent replacement by unmethylated cytosine by the base excision repair system. Methylation at the C5 position of cytosine bases is an epigenetic modification of the mammalian genome which plays an important role in transcriptional regulation. TET1 preferentially binds to CpG-rich sequences at promoters of both transcriptionally active and polycomb-repressed genes. By controlling the levels of 5mC and 5hmC at gene promoters, it may regulate the gene expression silencing induced by cytosine methylation. May have a dual function by also repressing the expression of a subset of genes through recruitment of transcriptional repressors to promoters.
Product Categories/Family for anti-TET1 antibody
NCBI and Uniprot Product Information
Molecular Weight
215kDa
Protein Family
Similar Products
Product Notes
The TET1 (Catalog #AAA389455) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The AbFlex TET1 antibody (rAb) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TET1 can be used in a range of immunoassay formats including, but not limited to, Chromatin Immunoprecipitation-Seq (ChIP-Seq). Applications Validated: ChIP-Seq: 4ul per ChIP AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone. Researchers should empirically determine the suitability of the TET1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TET1, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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