Human Exosome Component 2 (EXOSC2) RTU ELISA Kit | EXOSC2 rtu elisa kit

Human Exosome Component 2 (EXOSC2) ELISA Kit

Cat. Num. AAA459128

• Gene Names: EXOSC2; p7; RRP4; SHRF; Rrp4p; hRrp4p
• Reactivity: Human
• Synonyms: Exosome Component 2 (EXOSC2); Human Exosome Component 2 (EXOSC2) ELISA Kit; RRP4; Rrp4p; hRrp4p; p7; Ribosomal RNA-processing protein 4; EXOSC2 rtu elisa kit

• Reactivity: Human
• Sequence Length: 267

• Assay Type: Sandwich
• Samples: Tissue Homogenates, Cell Lysates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.061ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for EXOSC2 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to EXOSC2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to EXOSC2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain EXOSC2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of EXOSC2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 544185998
• NCBI GeneID: 23404
• NCBI Accession #: NP_001269637.1
• NCBI GenBank Nucleotide #: NM_001282708.1
• UniProt Accession #: Q13868; Q9NUY4; A3KFL3; A3KFL4; B4DKK6
• Molecular Weight: 29,408 Da
• NCBI Official Full Name: exosome complex component RRP4 isoform 2
• NCBI Official Synonym Full Names: exosome component 2
• NCBI Official Symbol: EXOSC2
• NCBI Official Synonym Symbols: p7; RRP4; SHRF; Rrp4p; hRrp4p
• NCBI Protein Information: exosome complex component RRP4
• UniProt Protein Name: Exosome complex component RRP4
• UniProt Gene Name: EXOSC2
• UniProt Synonym Gene Names: RRP4

Uniprot Description

Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. EXOSC2 as peripheral part of the Exo-9 complex stabilizes the hexameric ring of RNase PH-domain subunits through contacts with EXOSC4 and EXOSC7.