Dot Blot (DB)
(Dot blot of AbFlex 5-methylcytosine antibody (rAb). DNA standards from (5, 1 and 0.2 ng as indicated) were spotted onto positively charged nylon membrane and blotted with AbFlex 5-methylcytosine recombinant antibody at a 1 ug/mL. Lane 1: Unmethylated DNA. Lane 2: DNA containing 5-methylcytosine. Lane 3: DNA containing 5-hydroxymethylcytosine.)
Mouse 5-methylcytosine Antibody
AbFlex 5-methylcytosine antibody (rAb)
Reactivity
Not Species Specific
Applications
Dot Blot
Purity
Protein A Chromatography
Synonyms
5-methylcytosine; AbFlex 5-methylcytosine antibody (rAb); anti-5-methylcytosine antibody
Host
Mouse
Reactivity
Not Species Specific
Isotype
IgG2a
Purity/Purification
Protein A Chromatography
Form/Format
Purified IgG in 140mM Hepes, pH7.5, 70mM NaCl, 32mM NaOAc, 0.035% sodium azide, 30% glycerol. Sodium azide is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-5-methylcytosine antibody
Dot Blot (DB)
Application Notes
Applications Validated:
DB: 1ug/ml
DB: 1ug/ml
Antibody Type
Recombinant Antibody
Conjugation
Methylated
Immunogen
This antibody was raised against 5-methylcytosine conjugated to KLH.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Dot Blot (DB)
(Dot blot of AbFlex 5-methylcytosine antibody (rAb). DNA standards from (5, 1 and 0.2 ng as indicated) were spotted onto positively charged nylon membrane and blotted with AbFlex 5-methylcytosine recombinant antibody at a 1 ug/mL. Lane 1: Unmethylated DNA. Lane 2: DNA containing 5-methylcytosine. Lane 3: DNA containing 5-hydroxymethylcytosine.)
Dot Blot (DB)
(Dot blot of AbFlex 5-methylcytosine antibody (rAb). DNA standards from (5, 1 and 0.2 ng as indicated) were spotted onto positively charged nylon membrane and blotted with AbFlex 5-methylcytosine recombinant antibody at a 1 ug/mL. Lane 1: Unmethylated DNA. Lane 2: DNA containing 5-methylcytosine. Lane 3: DNA containing 5-hydroxymethylcytosine.)
Related Product Information for anti-5-methylcytosine antibody
Short Description: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. Our AbFlex 5-methylcytosine antibody (rAb) was raised in a Mouse host. It has been validated for use in Dot blot; the sequence is not species specific so it should react with a wide range of sample types.
Background: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex 5-methylcytosine (5-mC) antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. 5-Methylcytosine (5-Methylcytidine) is a modified base that is found in the DNA of plants and vertebrates. DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. This antibody is useful for discrimination between the modified base and its normal cytosine counterpart, allowing for gene promoter methylation analysis.
Background: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex 5-methylcytosine (5-mC) antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. 5-Methylcytosine (5-Methylcytidine) is a modified base that is found in the DNA of plants and vertebrates. DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. This antibody is useful for discrimination between the modified base and its normal cytosine counterpart, allowing for gene promoter methylation analysis.
Product Categories/Family for anti-5-methylcytosine antibody
NCBI and Uniprot Product Information
Protein Family
Similar Products
Product Notes
The 5-methylcytosine (Catalog #AAA389458) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The AbFlex 5-methylcytosine antibody (rAb) reacts with Not Species Specific and may cross-react with other species as described in the data sheet. AAA Biotech's 5-methylcytosine can be used in a range of immunoassay formats including, but not limited to, Dot Blot (DB). Applications Validated: DB: 1ug/ml. Researchers should empirically determine the suitability of the 5-methylcytosine for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "5-methylcytosine, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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