Dot Blot (DB)
(AbFlex N6-Methyladenosine (m6A) antibody tested by Dot blot. 3.3 ng of single-stranded DNA oligonucleotides were spotted on to a positively charged nylon membrane and blotted with AbFlex N-6-methyladenosine recombinant antibody (1 ug/ml dilution). 1. unmethylated sequence 1 2. single 6-methyl-adenosine in middle of sequence 1 3. unmethylated sequence 2 4. unmethylated sequence 3 5. single 1-methyl-adenosine in middle of sequence 3)
Mouse anti-Human, Mouse N6-Methyladenosine (m6A) Antibody | anti-m6A antibody
AbFlex N6-Methyladenosine (m6A) antibody (rAb)
Reactivity
Human, Mouse
Applications
Dot Blot, Immunoprecipitation
Purity
Protein A Chromatography
Synonyms
N6-Methyladenosine (m6A); AbFlex N6-Methyladenosine (m6A) antibody (rAb); anti-m6A antibody
Host
Mouse
Reactivity
Human, Mouse
Isotype
IgG2a
Purity/Purification
Protein A Chromatography
Form/Format
Purified IgG in 140mM Hepes, pH7.5, 70mM NaCl, 32mM NaOAc, 0.035% sodium azide, 30% glycerol. Sodium azide is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-m6A antibody
Dot Blot (DB), Immunoprecipitation (IP)
Application Notes
Applications Validated:
DB IP: 5-10ug per IP
DB IP: 5-10ug per IP
Antibody Type
Recombinant Antibody
Conjugation
Methylated
Immunogen
This antibody was raised against N6-methyladenosine conjugated to BSA.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Dot Blot (DB)
(AbFlex N6-Methyladenosine (m6A) antibody tested by Dot blot. 3.3 ng of single-stranded DNA oligonucleotides were spotted on to a positively charged nylon membrane and blotted with AbFlex N-6-methyladenosine recombinant antibody (1 ug/ml dilution). 1. unmethylated sequence 1 2. single 6-methyl-adenosine in middle of sequence 1 3. unmethylated sequence 2 4. unmethylated sequence 3 5. single 1-methyl-adenosine in middle of sequence 3)
Dot Blot (DB)
(AbFlex N6-Methyladenosine (m6A) antibody tested by Dot blot. 3.3 ng of single-stranded DNA oligonucleotides were spotted on to a positively charged nylon membrane and blotted with AbFlex N-6-methyladenosine recombinant antibody (1 ug/ml dilution). 1. unmethylated sequence 1 2. single 6-methyl-adenosine in middle of sequence 1 3. unmethylated sequence 2 4. unmethylated sequence 3 5. single 1-methyl-adenosine in middle of sequence 3)
Immunoprecipitation (IP)
(AbFlex N6-Methyladenosine (m6A) antibody tested by RNA immunoprecipitation. (A) In vitro RNA transcripts were generated containing either 0 or 10% of the m6A modified base or the A unmodified base and were spiked into 10 ug of total RNA. IP was performed using 10 ug Abflex N6-Methyladenosine antibody followed by Real Time-qPCR. (B) IP was performed using 5 ug Abflex N6-Methyladenosine antibody and 30 ug of total RNA (fragmented to 200 bp) from human hematopoetic stem cells followed by Real Time-qPCR on known methylated RNA. Data was provided courtesy of Dr. Alexandra Patmanidi in Prof. Dr. Achim Leutz’s lab, Max-Delbrück-Centrum für Molekulare Medizin (MDC).)
Immunoprecipitation (IP)
(AbFlex N6-Methyladenosine (m6A) antibody tested by RNA immunoprecipitation. (A) In vitro RNA transcripts were generated containing either 0 or 10% of the m6A modified base or the A unmodified base and were spiked into 10 ug of total RNA. IP was performed using 10 ug Abflex N6-Methyladenosine antibody followed by Real Time-qPCR. (B) IP was performed using 5 ug Abflex N6-Methyladenosine antibody and 30 ug of total RNA (fragmented to 200 bp) from human hematopoetic stem cells followed by Real Time-qPCR on known methylated RNA. Data was provided courtesy of Dr. Alexandra Patmanidi in Prof. Dr. Achim Leutz’s lab, Max-Delbrück-Centrum für Molekulare Medizin (MDC).)
Related Product Information for anti-m6A antibody
Short Description: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. Our AbFlex N6-Methyladenosine (m6A) antibody (rAb) was raised in a Mouse host. It has been validated for use in Dot blot and Immunoprecipitation; it has been shown to react with Human and Mouse samples, but the sequence is not species specific so it should react with a wide range of sample types.
Background: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex N6-Methyladenosine (m6A) antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. N6-Methyladenosine (m6A) is an RNA modification on the N-6 position of adenosine. This modification has been found to be abundant in the 3' UTR and stop codons of mammalian mRNA. m6A is associated with miRNA binding sites suggesting a potential role in epigenetic gene regulation. FTO and ALKBH are demethylases for 6-methyladenosine while a multiprotein complex that includes METTL3 functions as the methyltransferase. Recent findings revealed that m6A is also present on metazoan DNA, suggesting a genuine epigenetic role for this modification in the context of DNA as well.
Background: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex N6-Methyladenosine (m6A) antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. N6-Methyladenosine (m6A) is an RNA modification on the N-6 position of adenosine. This modification has been found to be abundant in the 3' UTR and stop codons of mammalian mRNA. m6A is associated with miRNA binding sites suggesting a potential role in epigenetic gene regulation. FTO and ALKBH are demethylases for 6-methyladenosine while a multiprotein complex that includes METTL3 functions as the methyltransferase. Recent findings revealed that m6A is also present on metazoan DNA, suggesting a genuine epigenetic role for this modification in the context of DNA as well.
Product Categories/Family for anti-m6A antibody
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Product Notes
The m6A (Catalog #AAA389488) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The AbFlex N6-Methyladenosine (m6A) antibody (rAb) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's N6-Methyladenosine (m6A) can be used in a range of immunoassay formats including, but not limited to, Dot Blot (DB), Immunoprecipitation (IP). Applications Validated: DB IP: 5-10ug per IP. Researchers should empirically determine the suitability of the m6A for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "N6-Methyladenosine (m6A), Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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