Western Blot (WB)
(Western blot analysis of a human recombinant GSK3beta N-terminal fragment (lanes 1 & 2) and endogenous GSK3beta expressed in mouse brain (lanes 3 & 4). The blot was probed with mouse monoclonal anti-GSK-3beta at 1:500 (lanes 1 & 3) and 1:2000 (lanes 2 & 4).)
GSK-3beta recombinant protein
GSK-3beta Recombinant Protein
Applications
Western Blot
Synonyms
GSK-3beta; GSK-3beta Recombinant Protein; GSK-3beta recombinant protein
Host
Lysate
Form/Format
GSK-3beta recombinant protein lysate is supplied at a concentration of 10ng/ul in electrophoresis sample buffer (62.5mM Tris pH6.8, 2% SDS, 5% glycerol, 0.003% bromophenol blue, 0.9% beta-mercaptoethanol).
Applicable Applications for GSK-3beta recombinant protein
Western Blot (WB)
Application Notes
WB: 10ul/lane
Preparation and Storage
Store at -20 degree C. Do not boil or dilute. Stable for 1 year.
Western Blot (WB)
(Western blot analysis of a human recombinant GSK3beta N-terminal fragment (lanes 1 & 2) and endogenous GSK3beta expressed in mouse brain (lanes 3 & 4). The blot was probed with mouse monoclonal anti-GSK-3beta at 1:500 (lanes 1 & 3) and 1:2000 (lanes 2 & 4).)
Western Blot (WB)
(Western blot analysis of a human recombinant GSK3beta N-terminal fragment (lanes 1 & 2) and endogenous GSK3beta expressed in mouse brain (lanes 3 & 4). The blot was probed with mouse monoclonal anti-GSK-3beta at 1:500 (lanes 1 & 3) and 1:2000 (lanes 2 & 4).)
Related Product Information for GSK-3beta recombinant protein
Two GSK-3 genes (alpha and beta) have been cloned in mammals and these kinase homologues show strong sequence conservation within their catalytic domain. GSK-3beta plays a critical role in cell survival by phosphorylating nuclear factor-kappaB (NF-kappaB) p65 subunit, leading to NF-kappaB transactivation in hepatocytes. Phosphorylation regulates the activity of both GSK-3 genes. MEK1/2 can phosphorylate tyrosine 216 (tyrosine 279 in GSK-3alpha), which stimulates GSK-3 kinase activity. Tyr-216 phosphorylation is required for GSK-mediated down-regulation of beta-catenin activity. Also, TRAIL stimulation can increase Tyr-216 phosphorylation, and GSK-3beta activity may suppress TRAIL-induced apoptosis. Inactiviation of GSK-3 occurs through Akt phosphorylation of serine 9 of GSK-3beta (Serine 21 in GSK-3alpha). This phosphorylation may be involved in later phases of neuronal apoptosis.Human recombinant GSK-3beta protein is a 20kDa fragment that includes amino acids 2 to 159. This GSK-3beta protein fragment is detected by mouse monoclonal anti-GSK-3beta (N-terminal region).
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Product Notes
The GSK-3beta (Catalog #AAA474369) is a Recombinant Protein produced from Lysate and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's GSK-3beta can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 10ul/lane. Researchers should empirically determine the suitability of the GSK-3beta for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GSK-3beta, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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