Rat Adrenergic Receptor Beta Kinase 1 ELISA Kit | ADRbetaK1 elisa kit

Rat Adrenergic Receptor Beta Kinase 1 ELISA Kit

Cat. Num. AAA762602

• Gene Names: ADRBK1; GRK2; BARK1; BETA-ARK1
• Reactivity: Rat
• Synonyms: Adrenergic Receptor Beta Kinase 1; Rat Adrenergic Receptor Beta Kinase 1 ELISA Kit; ADRbK1; ADRbetaK1 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of ADRbetaK1. No significant cross-reactivity or interference between ADRbetaK1 and analogues was observed.
• Sequence Length: 659

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ADR betaK1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ADR betaK1 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ADRbetaK1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- ADRbeta K1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- ADRbetaK1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the ADRbetaK1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of ADRbetaK1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 119594988
• NCBI GeneID: 156
• Molecular Weight: 79,574 Da
• NCBI Official Full Name: adrenergic, beta, receptor kinase 1, isoform CRA_b
• NCBI Official Synonym Full Names: adrenergic, beta, receptor kinase 1
• NCBI Official Symbol: ADRBK1
• NCBI Official Synonym Symbols: GRK2; BARK1; BETA-ARK1
• NCBI Protein Information: beta-adrenergic receptor kinase 1
• UniProt Protein Name: Beta-adrenergic receptor kinase 1
• UniProt Gene Name: ADRBK1
• UniProt Synonym Gene Names: BARK; BARK1; GRK2; Beta-ARK-1
• UniProt Entry Name: ARBK1_HUMAN

NCBI Description

The product of this gene phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. This protein is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart. [provided by RefSeq, Jul 2008]

Uniprot Description

GRK2: a ubiquitous protein kinase of the GRK family. Phosphorylates the beta-2-adrenergic receptor and related G-protein-coupled receptors. Appears to mediate agonist-specific desensitization observed at high agonist concentrations. Expression level is consistently elevated in chronic human heart failure. Mouse models of severe heart failure have been used to demonstrate that inhibition of BARK1 with a peptide inhibitor is sufficient to increase mean survival, reduce dialation and improve cardiac function.

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, AGC; EC 2.7.11.15; AGC group; GRK family; BARK subfamily

Chromosomal Location of Human Ortholog: 11q13.1

Cellular Component: cytoplasm; cytosol; membrane; plasma membrane

Molecular Function: alpha-2A adrenergic receptor binding; ATP binding; beta-adrenergic receptor kinase activity; Edg-2 lysophosphatidic acid receptor binding; G-protein coupled receptor kinase activity; protein binding; protein kinase activity

Biological Process: acetylcholine receptor signaling, muscarinic pathway; cardiac muscle contraction; desensitization of G-protein coupled receptor protein signaling pathway; entry of virus into host cell; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; heart development; innate immune response; negative regulation of striated muscle contraction; negative regulation of the force of heart contraction by chemical signal; nerve growth factor receptor signaling pathway; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; phospholipase C activation; positive regulation of catecholamine secretion; receptor internalization; signal transduction; tachykinin signaling pathway; viral genome replication