Mouse anti-Human TWIST Antibody | anti-TWIST antibody

AbFlex TWIST antibody (rAb)

Cat. Num. AAA389516

• Gene Names: TWIST1; CRS; CSO; SCS; ACS3; CRS1; BPES2; BPES3; SWCOS; TWIST; bHLHa38
• Reactivity: Human
• Applications: Chromatin Immunoprecipitation, Immunoprecipitation, Chromatin Immunoprecipitation, Immunoprecipitation, Western Blot
• Purity: Protein A Chromatography
• Synonyms: TWIST; AbFlex TWIST antibody (rAb); CRS; SCS; ACS3; CRS1; BPES2; BPES3; TWIST1; bHLHa38; anti-TWIST antibody

• Host: Mouse
• Reactivity: Human
• Isotype: IgG2a
• Purity/Purification: Protein A Chromatography
• Form/Format: 140mM Hepes, pH7.5, 70mM NaCl, 32mM NaOAc, 0.035% sodium azide, and 30% glycerol. Sodium azide is highly toxic.
• Concentration: 1ug/ul (varies by lot)

• Applicable Applications for anti-TWIST antibody: Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation-Seq (ChIP-Seq), Western Blot (WB)
• Application Notes: Applications Validated:; ChIP-Seq: 4ug per ChIP; WB: 2-4ug/ml; Note: Endogenous expression of TWIST is low in many cell lines so that more cell lysate and/or more Antibody may be required for detection in WB. The addition of Tween can also improve sensitivity (tested at 0.05%); however, but other bands at higher molecular weight may be detected using this method.
• Antibody Type: Recombinant Antibody
• Immunogen: This antibody was raised against a recombinant protein corresponding to full length mouse TWIST1 (twist homolog 1 (Drosophila)).
• Preparation and Storage: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul

SDS-Page

(AbFlex TWIST Antibody (rAb) tested by Western blot 50 ug of SH-SY5Y nuclear extract extract was run on SDS-PAGE and probed with antibody at 2 ug/ml.)

Chromatin Immunoprecipitation (ChIP)

(AbFlex TWIST Antibody (rAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from human brain tissue and 4 ug of antibody. ChIP DNA was sequenced on the Illumina HiSeq and sequence tags were mapped to identify TWIST binding sites across a region of chromosome 12.)

Related Product Information for anti-TWIST antibody

Short Description: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. Our AbFlex TWIST antibody (rAb) was raised in a Mouse host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq and Western blot; it has been shown to react with Human samples.

Background: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex TWIST antibody was expressed in was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. TWIST is a regulator of transcription that serves as a master regulator of embryonic morphogenesis. Through its bHLH motif it interacts with many different transcription factors to affect multiple pathways of gene expression. TWIST plays a pivotal role in mesodermal, myoblast, and osteoblast differentiation. TWIST is involved in the epithelial-mesenchymal transition (EMT), a period during embryonic development characterized by loss of cell adhesion and increased cell mobility, essential events for mesoderm and neural tube formation. The EMT shares many features of metastatic cancer, and elevated levels of TWIST are observed in many types of cancer. Mutations in the gene encoding TWIST have been associated with Saethre-Chotzen syndrome, an inherited developmental disorder characterized by skull deformations, limb abnormalities, and facial dysmorphisim.

Product Categories/Family for anti-TWIST antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 4507741
• NCBI GeneID: 7291
• NCBI Accession #: NP_000465
• NCBI GenBank Nucleotide #: NM_000474
• UniProt Accession #: Q15672
• Molecular Weight: 22kDa
• NCBI Official Full Name: twist-related protein 1
• NCBI Official Synonym Full Names: twist family bHLH transcription factor 1
• NCBI Official Symbol: TWIST1
• NCBI Official Synonym Symbols: CRS; CSO; SCS; ACS3; CRS1; BPES2; BPES3; SWCOS; TWIST; bHLHa38
• NCBI Protein Information: twist-related protein 1
• UniProt Protein Name: Twist-related protein 1
• Protein Family: Twist-related protein
• UniProt Gene Name: TWIST1
• UniProt Synonym Gene Names: BHLHA38; TWIST; bHLHa38
• UniProt Entry Name: TWST1_HUMAN

NCBI Description

This gene encodes a basic helix-loop-helix (bHLH) transcription factor that plays an important role in embryonic development. The encoded protein forms both homodimers and heterodimers that bind to DNA E box sequences and regulate the transcription of genes involved in cranial suture closure during skull development. This protein may also regulate neural tube closure, limb development and brown fat metabolism. This gene is hypermethylated and overexpressed in multiple human cancers, and the encoded protein promotes tumor cell invasion and metastasis. Mutations in this gene cause Saethre-Chotzen syndrome in human patients, which is characterized by craniosynostosis, ptosis and hypertelorism. [provided by RefSeq, Aug 2017]