Rabbit anti-Human PREX1 Polyclonal Antibody | anti-PREX1 antibody

Anti-PREX1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8101047

• Gene Names: PREX1; P-REX1
• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: PREX1; Polyclonal Antibody; Anti-PREX1 Antibody; Rabbit Polyclonal; PREX1 Antibody; Rabbit PAb; Antigen Affinity Purified; phosphatidylinositol-3; 4; 5-trisphosphate-dependent Rac exchange factor 1; Anti-P-REX1 Antibody; anti-PREX1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human PREX1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-PREX1 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IP: 1-4uL/mg of lysate
• Immunogen: A synthetic peptide corresponding to the center region of the human PREX1
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the human PREX1, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-PREX1 rabbit polyclonal antibody at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lane B: 293T Whole Cell Lysate Lane C: Raji Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:35 kDa Observed band size:42 kDa)

Immunoprecipitation (IP)

(ZFP42 was immunoprecipitated using: Lane A:0.5 mg Raw264.7 Whole Cell Lysate 2 uL anti-ZFP42 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-ZFP42 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 35 kDa Observed band size: 42 kDa)

Related Product Information for anti-PREX1 antibody

PREX1 is a Rac guanine exchange factor that coordinates signaling inputs from G protein-coupled receptors and receptor tyrosine kinases (RTKs). PREX1 creates a positive feedback loop to drive RTK, phosphatidylinositol 3-kinase (PI3K)/AKT, and MEK/ERK signaling. High PREX1 levels predict sensitivity to PI3K inhibitors in breast cancer cells.

NCBI and Uniprot Product Information

• NCBI GI #: 34452732
• NCBI GeneID: 57580
• NCBI Accession #: NP_065871.2
• NCBI GenBank Nucleotide #: NM_020820.3
• UniProt Accession #: Q8TCU6; Q5JS95; Q5JS96; Q69YL2; Q7Z2L9; Q9BQH0; Q9BX55; Q9H4Q6; Q9P2D2; Q9UGQ4; E1P5X9
• Molecular Weight: 175,847 Da
• NCBI Official Full Name: phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein
• NCBI Official Synonym Full Names: phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 1
• NCBI Official Symbol: PREX1
• NCBI Official Synonym Symbols: P-REX1
• NCBI Protein Information: phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein; ptdIns(3,4,5)-dependent Rac exchanger 1
• UniProt Protein Name: Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein
• Protein Family: Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein
• UniProt Gene Name: PREX1
• UniProt Synonym Gene Names: KIAA1415; P-Rex1; PtdIns(3,4,5)-dependent Rac exchanger 1
• UniProt Entry Name: PREX1_HUMAN

NCBI Description

The protein encoded by this gene acts as a guanine nucleotide exchange factor for the RHO family of small GTP-binding proteins (RACs). It has been shown to bind to and activate RAC1 by exchanging bound GDP for free GTP. The encoded protein, which is found mainly in the cytoplasm, is activated by phosphatidylinositol-3,4,5-trisphosphate and the beta-gamma subunits of heterotrimeric G proteins. [provided by RefSeq, Jul 2008]

Uniprot Description

PREX1: Functions as a RAC guanine nucleotide exchange factor (GEF), which activates the Rac proteins by exchanging bound GDP for free GTP. Its activity is synergistically activated by phosphatidylinositol 3,4,5-trisphosphate and the beta gamma subunits of heterotrimeric G protein. May function downstream of heterotrimeric G proteins in neutrophils. Interacts preferentially with RAC2. Interacts with RAC1. Mainly expressed in peripheral blood leukocytes and brain. Expressed at intermediate level in spleen and lymph nodes, and weakly expressed in other tissues. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: GEFs; GEFs, Rac/Rho

Chromosomal Location of Human Ortholog: 20q13.13

Cellular Component: growth cone; intracellular membrane-bound organelle; perinuclear region of cytoplasm; cytoplasm; plasma membrane; dendritic shaft; cytosol

Molecular Function: Rho guanyl-nucleotide exchange factor activity; protein binding; enzyme binding; phospholipid binding

Biological Process: neutrophil chemotaxis; G-protein coupled receptor protein signaling pathway; regulation of actin filament polymerization; neutrophil activation; actin filament polymerization; superoxide metabolic process; regulation of dendrite development; T cell differentiation