Mouse CRP ELISA Kit | CRP elisa kit

Mouse CRP PicoKine ELISA Kit

Cat. Num. AAA177325

• Gene Names: Crp; AI255847
• Reactivity: Mouse
• Synonyms: CRP; Mouse CRP PicoKine ELISA Kit; C-reactive protein; pentraxin-related; C reactive protein pentraxin related; C-reactive protein(1-205); CRP_HUMAN; MGC149895; MGC88244; Pentraxin 1; Pentraxin 1 short; PTX 1; PTX1; CRP elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant Mouse Crp
• Sequence Length: 225

• Samples: Cell culture supernates, cell lysates, serum and plasma (heparin, edta)
• Detection Range: 156pg/ml-10000pg/ml
• Sensitivity: <10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess interassay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CRP elisa kit

Principle of the Assay: The Picokine™ Mouse Crp Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse Crp with a 96-well strip plate that is pre-coated with antibody specific for Crp. The detection antibody is a biotinylated antibody specific for Crp. The capture antibody is monoclonal antibody from rat, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Mouse Crp with immunogen: Expression system for standard: NSO; Immunogen sequence: H20-S225. The kit is analytically validated with ready to use reagents. To measure Mouse Crp, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Mouse Crp in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Crp in the sample.

Background/Introduction: C Reactive Protein(CRP) is a major acute phase reactant synthesized primarily in the liver hepatocytes. It is composed of 5 identical, 21,500-molecular weight subunits. CRP mediates activities associated with preimmune nonspecific host resistance. CRP shows the strongest association with cardiovascular events. It is detectable on the surface of about 4% of normal peripheral blood lymphocytes. Acute phase reactant CRP is produced in the liver.

NCBI and Uniprot Product Information

• NCBI GI #: 408360035
• NCBI GeneID: 12944
• UniProt Accession #: P14847; E9PZ20
• Molecular Weight: 25,360 Da
• NCBI Official Full Name: C-reactive protein
• NCBI Official Synonym Full Names: C-reactive protein, pentraxin-related
• NCBI Official Symbol: Crp
• NCBI Official Synonym Symbols: AI255847
• NCBI Protein Information: C-reactive protein; C-reactive protein, petaxin related
• UniProt Protein Name: C-reactive protein
• Protein Family: Cryparin
• UniProt Gene Name: Crp
• UniProt Synonym Gene Names: Ptx1
• UniProt Entry Name: CRP_MOUSE

Uniprot Description

CRP: Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. Belongs to the pentaxin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: extracellular space; growth cone; extracellular region; filopodium

Molecular Function: protein homodimerization activity; low-density lipoprotein receptor binding; metal ion binding; complement component C1q binding; low-density lipoprotein binding; cholesterol binding; calcium ion binding

Biological Process: protein polymerization; positive regulation of nitric oxide biosynthetic process; negative regulation of vasodilation; wound healing; positive regulation of superoxide release; acute-phase response; negative regulation of superoxide release; complement activation, classical pathway