Mouse Podocalyxin ELISA Kit | PODXL elisa kit

Mouse Podocalyxin PicoKine ELISA Kit

Cat. Num. AAA1752600

• Gene Names: Podxl; PC; Ly102; Pclp1; PCLP-1; Podxl1; AW121214
• Reactivity: Mouse
• Synonyms: Podocalyxin; Mouse Podocalyxin PicoKine ELISA Kit; Mouse Podocalyxin; podocalyxin-like; Podocalyxin-like protein 1; PC; PCLP-1; Podxl; Pclp1; PODXL elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant Mouse Podxl
• Sequence Length: 503

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Detection Range: 156pg/ml-10000pg/ml
• Sensitivity: <50pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PODXL elisa kit

Principle of the Assay: The Picokine™ Mouse Podxl Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse Podxl with a 96-well strip plate that is pre-coated with antibody specific for Podxl. The detection antibody is a biotinylated antibody specific for Podxl. The capture antibody is a monoclonal antibody from rat, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Mouse Podxl with immunogen: Expression system for standard: NSO, Immunogen sequence: H22-S404. The kit is analytically validated with ready to use reagents. To measure Mouse Podxl, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Mouse Podxl in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Podxl in the sample.!!Background/Introduction: Podocalyxin-like protein 1 is a protein that in humans is encoded by the PODXL gene. It is mapped to 6 A3.3; 6 12.57 Cm. This gene encodes a member of the CD34 sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Inactivation of the encoding gene in mice leads to anuria, omphalocele and perinatal death. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin.

NCBI and Uniprot Product Information

• NCBI GI #: 34328219
• NCBI GeneID: 27205
• NCBI Accession #: NP_038751.2
• NCBI GenBank Nucleotide #: NP_038751.2
• UniProt Accession #: Q9R0M4
• NCBI Official Full Name: podocalyxin
• NCBI Official Synonym Full Names: podocalyxin-like
• NCBI Official Symbol: Podxl
• NCBI Official Synonym Symbols: PC; Ly102; Pclp1; PCLP-1; Podxl1; AW121214
• NCBI Protein Information: podocalyxin
• UniProt Protein Name: Podocalyxin
• Protein Family: Podocalyxin
• UniProt Gene Name: Podxl
• UniProt Synonym Gene Names: Pclp1; PC; PCLP-1

Uniprot Description

PODXL: Involved in the regulation of both adhesion and cell morphology and cancer progression. Function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell- cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up inital epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR- dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells. Belongs to the podocalyxin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 6 A3.3|6 12.57 cM

Cellular Component: apical plasma membrane; microvillus membrane; plasma membrane

Biological Process: cell migration; leukocyte migration; negative regulation of cell adhesion; negative regulation of cell-cell adhesion; regulation of microvillus biogenesis