Typical Testing Data/Standard Curve (for reference only)
(Rat CRP PicoKine ELISA Kit standard curve )
Rat CRP ELISA Kit | CRP elisa kit
Rat CRP PicoKine ELISA Kit
Gene Names
Crp; Aa1249; Ac1262; Ab1-341; Ab2-196; Ac1-114; Ac2-069; Ba2-693
Reactivity
Rat
Synonyms
CRP; Rat CRP PicoKine ELISA Kit; C-reactive protein; pentraxin-related; C reactive protein pentraxin related; C-reactive protein(1-205); CRP_HUMAN; MGC149895; MGC88244; Pentraxin 1; Pentraxin 1 short; PTX 1; PTX1; CRP elisa kit
Reactivity
Rat
Specificity
Natural and recombinant rat CRP
Sequence Length
230
Samples
Cell culture supernates, serum and plasma (heparin, EDTA)
Assay Type
Sandwich
Detection Range
156pg/ml-10,000pg/ml
Sensitivity
< 10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Typical Testing Data/Standard Curve (for reference only)
(Rat CRP PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Rat CRP PicoKine ELISA Kit standard curve )
Related Product Information for CRP elisa kit
Principle of the assay: Rat CRP ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CRP has been precoated onto 96-well plates. Standards(NSO, H20-S230) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CRP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat CRP amount of sample captured in plate.
Background: C Reactive Protein (CRP) is a major acute phase reactant synthesized primarily in the liver hepatocytes. It is composed of 5 identical, 21,500-molecular weight subunits. CRP mediates activities associated with preimmune nonspecific host resistance. CRP shows the strongest association with cardiovascular events. It is detectable on the surface of about 4% of normal peripheral blood lymphocytes. Acute phase reactant CRP is produced in the liver.
Background: C Reactive Protein (CRP) is a major acute phase reactant synthesized primarily in the liver hepatocytes. It is composed of 5 identical, 21,500-molecular weight subunits. CRP mediates activities associated with preimmune nonspecific host resistance. CRP shows the strongest association with cardiovascular events. It is detectable on the surface of about 4% of normal peripheral blood lymphocytes. Acute phase reactant CRP is produced in the liver.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
25,468 Da
NCBI Official Full Name
C-reactive protein
NCBI Official Synonym Full Names
C-reactive protein, pentraxin-related
NCBI Official Symbol
Crp
NCBI Official Synonym Symbols
Aa1249; Ac1262; Ab1-341; Ab2-196; Ac1-114; Ac2-069; Ba2-693
NCBI Protein Information
C-reactive protein; C-reactive protein, petaxin related; C-reactive protein member of the pentraxin family; C-reactive protein, member of the pentraxin family
UniProt Protein Name
C-reactive protein
Protein Family
UniProt Gene Name
Crp
UniProt Synonym Gene Names
Ptx1
UniProt Entry Name
CRP_RAT
NCBI Description
glycoprotein of the acute phase response [RGD, Feb 2006]
Uniprot Description
Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells ().
Research Articles on CRP
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Product Notes
The Rat CRP crp (Catalog #AAA177287) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA177287 ELISA Kit recognizes Rat CRP. It is sometimes possible for the material contained within the vial of "CRP, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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