Mouse C-reactive protein, pentraxin-related ELISA Kit | CRP elisa kit

Mouse C-Reactive Protein, CRP ELISA Kit

Cat. Num. AAA700197

• Gene Names: Crp; AI255847
• Reactivity: Mouse
• Synonyms: C-reactive protein; pentraxin-related; Mouse C-Reactive Protein; CRP ELISA Kit; MGC149895; MGC88244; PTX1; ; CRP elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse CRP. No significant cross-reactivity or interference between Mouse CRP and analogues was observed.
• Sequence Length: 225

• Samples: Serum, plasma, cell culture supernates, tissue homogenates
• Detection Range: 15.6 ng/ml-1000 ng/ml
• Sensitivity: 3.9 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Immunity
• Protein Biological Process 3: Acute phase
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CRP elisa kit

Introduction: C-reactive protein (CRP) is a protein found in the blood inresponse to inflammation (an acute-phase protein). CRP issynthesized by the liver in response to factors released by fatcells (adipocytes). It is a member of the pentraxin family ofproteins. CRP is a member of the class of acute-phase reactantsas its levels rise dramatically during inflammatory processesoccurring in the body. This increment is due to a rise in theplasma concentration of IL-6, which is produced predominantlyby macrophages as well as adipocytes.CRP binds to phosphocholine on microbes. It is thought to assistin complement binding to foreign and damaged cells andenhances phagocytosis by macrophages, which express areceptor for CRP. It is also believed to play another importantrole in innate immunity, as an early defense system againstinfections.CRP rises up to 50,000-fold in acute inflammation, such asinfection. It rises above normal limits within 6 hours, and peaks at48 hours. Its half-life is constant, and therefore its level is mainlydetermined by the rate of production (and hence the severity ofthe precipitating cause). Serum amyloid A is a relatedacute-phase marker that responds rapidly in similarcircumstances.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated withan antibody specific to CRP. Standards or samples are thenadded to the appropriate microtiter plate wells with abiotin-conjugated antibody preparation specific for CRP andAvidin conjugated to Horseradish Peroxidase (HRP) is added toeach microplate well and incubated. Then a TMB (3,3',5,5'tetramethyl-benzidine) substrate solution is added to each well.Only those wells that contain CRP, biotin-conjugated antibodyand enzyme-conjugated Avidin will exhibit a change in color. Theenzyme-substrate reaction is terminated by the addition of asulphuric acid solution and the color change is measuredspectrophotometrically at a wavelength of 450 nm +/- 2 nm. Theconcentration of CRP in the samples is then determined bycomparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 162138926
• NCBI GeneID: 12944
• NCBI Accession #: NP_031794.3
• NCBI GenBank Nucleotide #: NM_007768.4
• UniProt Accession #: P14847; E9PZ20
• Molecular Weight: 25,360 Da
• NCBI Official Full Name: C-reactive protein
• NCBI Official Synonym Full Names: C-reactive protein, pentraxin-related
• NCBI Official Symbol: Crp
• NCBI Official Synonym Symbols: AI255847
• NCBI Protein Information: C-reactive protein; C-reactive protein, petaxin related
• UniProt Protein Name: C-reactive protein
• Protein Family: Cryparin
• UniProt Gene Name: Crp
• UniProt Synonym Gene Names: Ptx1
• UniProt Entry Name: CRP_MOUSE

Uniprot Description

Function: Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.

Cofactor: Binds 2 calcium ions per subunit