Testing Data
IgMC secondary antibody
Mouse Anti-Chicken IgM-APC
Reactivity
Chicken
Applications
Flow Cytometry, Functional Assay, ELISA, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Synonyms
IgMC; Mouse Anti-Chicken IgM-APC; IgMC secondary antibody
Host
Mouse
Reactivity
Chicken
Isotype
IgG2bkappa
Clone Number
M-1
Specificity
Chicken/Turkey IgM
Form/Format
Supplied in PBS/NaN3 and a stabilizing agent
Concentration
0.1 mg/mL (varies by lot)
Applicable Applications for IgMC secondary antibody
Flow Cytometry (FC/FACS), ELISA (EIA), Fluorescence-Linked Immunosorbent Assay (FLISA), Immunohistochemistry (IHC) Formalin, Immunocytochemistry (ICC), Immunoprecipitation (IP)
Volume
1.0 mL
Immunogen
Affinity purified chicken Ig or isolated lymphocytes
Conjugate
APC (Allophycocyanin)
Isotype Control
Mouse IgG2b-APC
Preparation and Storage
Store at 2-8 degree C.
Testing Data
Testing Data
References
1. Chen CH, Lehmeyer JE, Cooper MD. Evidence for an IgD homologue on chicken lymphocytes. J Immunol. 1982;129:2580-5. (Immunogen, IP, ICC). 2. Ekino S, Riwar B, Kroese FG, Schwander EH, Koch G, Nieuwenhuis P. Role of environmental antigen in the development of IgG+ cells in the bursa of fabricius. J Immunol. 1995;155:4551-8. (FC, IP, IHC-FS). 3. Schusser B, Collarini EJ, Yi H, Izquierdo SM, Fesler J, Pedersen D, et al. Immunoglobulin knockout chickens via efficient homologous recombination in primordial germ cells. Proc Natl Acad Sci USA. 2013;110:20170-5. (IHC-FS, FC). 4. Bader SR, Kothlow S, Trapp S, Schwarz SC, Philipp H, Weigend S, et al. Acute paretic syndrome in juvenile White Leghorn chickens resembles late stages of acute inflammatory demyelinating polyneuropathies in humans. J Neuroinflammation. 2010;7:7. (IHC-FS, FC). 5. Alinikula J, Nera K, Junttila S, Lassila O. Alternate pathways for Bcl6-mediated regulation of B cell to plasma cell differentiation. Eur J Immunol. 2011;41:2404-13. (IP). 6. Viertlboeck BC, Schweinsberg S, Hanczaruk MA, Schmitt R, Du Pasquier L, Herberg FW, et al. The chicken leukocyte receptor complex encodes a primordial, activating, high-affinity IgY Fc receptor. Proc Natl Acad Sci USA. 2007;104:11718-23. (Stim). 7. Wang YW, Cherian G, Sunwoo HH, Sim JS. Dietary polyunsaturated fatty acids significantly affect laying hen lymphocyte proliferation and immunoglobulin G concentration in serum and egg yolk. Can J Anim Sci. 2000;80:597-604. (FC). 8. Selvaraj RK, Cherian G. Dietary n-3 fatty acids reduce the delayed hypersensitivity reaction and antibody production more than n-6 fatty acids in broiler birds. Eur J Lipid Sci Technol. 2004;106:3-10. (FC). 9. Selvaraj RK, Cherian G. Changes in delayed type hypersensitivity, egg antibody content and immune cell fatty acid composition of layer birds fed conjugated linoleic acid, n-6 or n-3 fatty acids. Can J Anim Sci. 2004;84:221-8. (FC). 10. Chowdhury SR, Smith TK, Boermans HJ, Woodward B. Effects of feed-borne Fusarium mycotoxins on hematology and immunology of laying hens. Poult Sci. 2005;84:1841-50. (FC). 11. Nera K, Alinikula J, Terho P, Narvi E, Törnquist K, Kurosaki T, et al. Ikaros has a crucial role in regulation of B cell receptor signaling. Eur J Immunol. 2006;36:516-25. (FC). 12. Yang SY, Fugmann SD, Schatz DG. Control of gene conversion and somatic hypermutation by immunoglobulin promoter and enhancer sequences. J Exp Med. 2006;203:2919-28. (FC). 13. Cook AJ, Raftery JM, Lau KK, Jessup A, Harris RS, Takeda S, et al. DNA-dependent protein kinase inhibits AID-induced antibody gene conversion. PLoS Biol. 2007;5 (4):e80. (FC). 14. Yang SY, Fugmann SD, Gramlich HS, Schatz DG. Activation-induced cytidine deaminase-mediated sequence diversification is transiently targeted to newly integrated DNA substrates. J Biol Chem. 2007;282:25308-13. (FC). 15. Janardhana V, Broadway MM, Bruce MP, Lowenthal JW, Geier MS, Hughes RJ, et al. Prebiotics modulate immune responses in the gut-associated lymphoid tissue of chickens. J Nutr. 2009;139:1404-9. (FC). 16. Petkov DI, Linnemann EG, Kapczynski DR, Sellers HS. Identification and characterization of two distinct bursal B-cell subpopulations following infectious bursal disease virus infection of White Leghorn chickens. Avian Dis. 2009;53:347-55. (FC). 17. Luo J, Zhang H, Teng M, Fan J, You L, Xiao Z, et al. Surface IgM on DT40 cells may be a component of the putative receptor complex responsible for the binding of infectious bursal disease virus. Avian Pathol. 2010;39:359-65. (FC). 18. Jankowski J, Zdu?czyk Z, Sartowska K, Tyka?owski B, Stenzel T, Wróblewska M, et al. Metabolic and immune response of young turkeys originating from parent flocks fed diets with inorganic or organic selenium. Pol J Vet Sci. 2011;14:353-8. (FC, Turkey Reactivity). 19. Levkut M, Revajová V, Lauková A, Šev?íková Z, Spišáková V, Faixová Z, et al. Leukocytic responses and intestinal mucin dynamics of broilers protected with Enterococcus faecium EF55 and challenged with Salmonella Enteritidis. Res Vet Sci. 2012;93:195-201. (FC). 20. Revajová V, Slaminková Z, Grešáková L, Levkut M. Duodenal morphology and immune responses of broiler chickens fed low doses of deoxynivalenol. Acta Vet. Brno. 2013;82:337-42. (FC)
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Product Notes
The IgMC (Catalog #AAA679323) is a Secondary Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Chicken IgM-APC reacts with Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's IgMC can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA), Fluorescence-Linked Immunosorbent Assay (FLISA), Immunohistochemistry (IHC) Formalin, Immunocytochemistry (ICC), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the IgMC for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgMC, Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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