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Testing Data

Rat anti-Mouse MHC Class IIC Antibody

Rat Anti-Mouse MHC Class II-APC

Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Immunocytochemistry
Synonyms
MHC Class IIC; Rat Anti-Mouse MHC Class II-APC; I-A antigen; anti-MHC Class IIC antibody
Ordering
For Research Use Only!
Host
Rat
Reactivity
Mouse
Isotype
IgG2bkappa
Clone Number
NIMR-4
Specificity
Mouse MHC Class II
Form/Format
Supplied in PBS/NaN3 and a stabilizing agent
Concentration
0.1 mg/mL (varies by lot)
Applicable Applications for anti-MHC Class IIC antibody
Flow Cytometry (FC/FACS), Immunohistochemistry (IHC) Formalin, Immunocytochemistry (ICC)
Volume
1.0 mL
Conjugate
APC (Allophycocyanin)
Isotype Control
Rat IgG2b-APC
Preparation and Storage
Store at 2-8 degree C.

Testing Data

Testing Data
Related Product Information for anti-MHC Class IIC antibody
The monoclonal antibody NIMR-4 reacts specifically with a non-polymorphic I-A-encoded epitope on MHC Class II antigens. Class II antigens are predominantly expressed on antigen-presenting cells including B lymphocytes, macrophages, dendritic cells, and certain epithelial cells.
References
1. Andrew EM, Parkhouse RM. Immune induction of Ia antigens in activated T cells and in kidney epithelial cells in mice. Immunology. 1986;58:603-6. (IHC-FS, ICC). 2. Zaliauskiene L, Kang S, Sparks K, Zinn KR, Schwiebert LM, Weaver CT, et al. Enhancement of MHC class II-restricted responses by receptor-mediated uptake of peptide antigens. J Immunol. 2002;169:2337-45. (ICC). 3. Lindsey JW. Dexamethasone-induced Ras-related protein 1 is a potential regulatory protein in B lymphocytes. Int Immunol. 2007;19:583-90. (Sep). 4. Caspar-Bauguil S, Cousin B, Galinier A, Segafredo C, Nibbelink M, André M, et al. Adipose tissues as an ancestral immune organ: site-specific change in obesity. FEBS. 2005;579:3487-92. (FC). 5. Bauer C, Bauernfeind F, Sterzik A, Orban M, Schnurr M, Lehr HA, et al. Dendritic cell-based vaccination combined with gemcitabine increases survival in a murine pancreatic carcinoma model. Gut. 2007;56:1275-82. (FC). 6. Krishnamoorthy N, Oriss TB, Paglia M, Fei M, Yarlagadda M, Vanhaesebroeck B, et al. Activation of c-Kit in dendritic cells regulates T helper cell differentiation and allergic asthma. Nat Med. 2008;14:565-73. (FC). 7. Gu T, Zhu Y, Chen C, Li M, Chen Y, Yu G, et al. Fine-tuned expression of programmed death 1 ligands in mature dendritic cells stimulated by CD40 ligand is critical for the induction of an efficient tumor specific immune response. Cell Mol Immunol. 2008;5:33-9. (FC). 8. Brix S, Lund P, Kjaer TM, Straarup EM, Hellgren LI, Frøkiær H. CD4+ T-cell activation is differentially modulated by bacteria-primed dendritic cells, but is generally down-regulated by n-3 polyunsaturated fatty acids. Immunology. 2010;129:338-50. (FC). 9. Jacobsen JT, Lunde E, Sundvold-Gjerstad V, Munthe LA, Bogen B. The cellular mechanism by which complementary Id+ and anti-Id antibodies communicate: T cells integrated into idiotypic regulation. Immunol Cell Biol. 2010;88:515-22. (FC). 10. Arora M, Poe SL, Oriss TB, Krishnamoorthy N, Yarlagadda M, Wenzel SE, et al. TLR4/MyD88-induced CD11b+Gr-1intF4/80+ non-migratory myeloid cells suppress Th2 effector function in the lung. Mucosal Immunol. 2010;3:578-93. (FC). 11. Duewell P, Kisser U, Heckelsmiller K, Hoves S, Stoitzner P, Koernig S, et al. ISCOMATRIX adjuvant combines immune activation with antigen delivery to dendritic cells in vivo leading to effective cross-priming of CD8+ T cells. J Immunol. 2011;187:55-63. (FC). 12. Szyszko EA, Skarstein K, Jonsson R, Brokstad KA. Distinct phenotypes of plasma cells in spleen and bone marrow of autoimmune NOD.B10.H2b mice. Autoimmunity. 2011;44:415-26. (FC). 13. Khare A, Krishnamoorthy N, Oriss TB, Fei M, Ray P, Ray A. Cutting edge: inhaled antigen upregulates retinaldehyde dehydrogenase in lung CD103+ but not plasmacytoid dendritic cells to induce Foxp3 de novo in CD4+ T cells and promote airway tolerance. J Immunol. 2013;191:25-29. (FC)

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Product Notes

The MHC Class IIC (Catalog #AAA679285) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Rat Anti-Mouse MHC Class II-APC reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MHC Class IIC can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunohistochemistry (IHC) Formalin, Immunocytochemistry (ICC). Researchers should empirically determine the suitability of the MHC Class IIC for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MHC Class IIC, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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