Mouse anti-Porcine CD3eC Antibody | anti-CD3eC antibody

Mouse Anti-Porcine CD3e-APC

Cat. Num. AAA679302

• Reactivity: Porcine
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Immunoprecipitation, Immunoblot, Western Blot
• Synonyms: CD3eC; Mouse Anti-Porcine CD3e-APC; anti-CD3eC antibody

• Host: Mouse
• Reactivity: Porcine
• Isotype: IgG1kappa
• Clone Number: PPT3
• Specificity: Porcine CD3epsilon
• Form/Format: Supplied in PBS/NaN3 and a stabilizing agent
• Concentration: 0.1 mg/mL (varies by lot)

• Applicable Applications for anti-CD3eC antibody: Flow Cytometry (FC/FACS), Immunohistochemistry (IHC) Formalin/Paraffin, Immunoprecipitation (IP), Immunoblotting (IB), Separation, Stimulation, Cytoxicity
• Volume: 1.0 mL
• Immunogen: Purified CD3 molecules from porcine thymus
• Conjugate: APC (Allophycocyanin)
• Isotype Control: Mouse IgG1-APC
• Preparation and Storage: Store at 2-8 degree C.

Testing Data

Related Product Information for anti-CD3eC antibody

Porcine CD3epsilon is a member of the T-cell receptor-associated CD3 complex. It is found on a subpopulation of thymocytes and on all pig T lymphocytes. The monoclonal antibody PPT3 is mitogenic when presented to peripheral blood mononuclear cells in immobilized form.

References

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Jung K, Alekseev KP, Zhang X, Cheon D, Vlasova AN, Saif LJ. Altered pathogenesis of porcine respiratory coronavirus in pigs due to immunosuppressive effects of dexamethasone: implications for corticosteroid use in treatment of severe acute respiratory syndrome coronavirus. J Virol. 2007;81:13681-93. (IHC-PS). 7. Isling LK, Aalbæk B, Schrøder M, Leifsson PS. Pyelonephritis in slaughter pigs and sows: morphological characterization and aspects of pathogenesis and aetiology. Acta Vet Scand. 2010;52:48. (IHC-PS). 8. Toka FN, Nfon CK, Dawson H, Golde WT. Accessory-cell-mediated activation of porcine NK cells by toll-like receptor 7 (TLR7) and TLR8 agonists. Clin Vaccine Immunol. 2009;16:866-78. (Sep). 9. Toka FN, Nfon CK, Dawson H, Estes DM, Golde WT. Activation of porcine natural killer cells and lysis of foot-and-mouth disease virus infected cells. J Interferon Cytokine Res. 2009;29:179-92. (Sep). 10. Toka FN, Nfon C, Dawson H, Golde WT. Natural killer cell dysfunction during acute infection with foot-and-mouth disease virus. Clin Vaccine Immunol. 2009;16:1738-49. (Sep). 11. Wen K, Bui T, Li G, Liu F, Li Y, Kocher J, et al. Characterization of immune modulating functions of gammadelta T cell subsets in a gnotobiotic pig model of human rotavirus infection. Comp Immunol Microbiol Infect Dis. 2012;35:289-301. (FC, Sep). 12. Liu KY, Comstock SS, Shunk JM, Monaco MH, Donovan SM. Natural killer cell populations and cytotoxic activity in pigs fed mother's milk, formula, or formula supplemented with bovine lactoferrin. Pediatr Res. 2013;74:402-7. (FC, Sep). 13. Lohse L, Nielsen J, Eriksen L. Temporary CD8+ T-cell depletion in pigs does not exacerbate infection with porcine reproductive and respiratory syndrome virus (PRRSV). Viral Immunol. 2004;17:594-603. (FC). 14. Davis ME, Maxwell CV, Erf GF, Brown DC, Wistuba TJ. 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Facci MR, Auray G, Buchanan R, van Kessel J, Thompson DR, Mackenzie-Dyck S, et al. A comparison between isolated blood dendritic cells and monocyte-derived dendritic cells in pigs. Immunology. 2010;129:396-405. (FC). 19. Martelli P, Gozio S, Ferrari L, Rosina S, De Angelis E, Quintavalla C, et al. Efficacy of a modified live porcine reproductive and respiratory syndrome virus (PRRSV) vaccine in pigs naturally exposed to a heterologous European (Italian cluster) field strain: Clinical protection and cell-mediated immunity. Vaccine. 2009;27:3788-99. (FC). 20. Diaz-San Segundo F, Moraes MP, de Los Santos T, Dias CC, Grubman MJ. Interferon-induced protection against foot-and-mouth disease virus infection correlates with enhanced tissue-specific innate immune cell infiltration and interferon-stimulated gene expression. J Virol. 2010;84:2063-77. (IHC-FS). 21. Ferrari L, Borghetti P, Gozio S, De Angelis E, Ballotta L, Smeets J, et al. Evaluation of the immune response induced by intradermal vaccination by using a needle-less system in comparison with the intramuscular route in conventional pigs. Res Vet Sci. 2011;90:64-71. (FC). 22. Oh Y, Seo HW, Han K, Park C, Chae C. Protective effect of the maternally derived porcine circovirus type 2 (PCV2)-specific cellular immune response in piglets by dam vaccination against PCV2 challenge. J Gen Virol. 2012;93:1556-62. (FC). 23. Chattha KS, Vlasova AN, Kandasamy S, Rajashekara G, Saif LJ. Divergent immunomodulating effects of probiotics on T cell responses to oral attenuated human rotavirus vaccine and virulent human rotavirus infection in a neonatal gnotobiotic piglet disease model. J Immunol. 2013;191:2446-56. (FC). 24. Liu P, Pieper R, Tedin L, Martin L, Meyer W, Rieger J, et al. Effect of dietary zinc oxide on jejunal morphological and immunological characteristics in weaned piglets. J Anim S. 2014;92:5009-18. (FC). 25. 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